Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | 1 mM, 1.75fold increase in activity | Digitalis lanata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activates at 1 mM, inhibitory at 10 mM | Digitalis lanata | |
ascorbate | - |
Digitalis lanata | |
Ca2+ | - |
Digitalis lanata | |
Co2+ | - |
Digitalis lanata | |
L-cysteine | 1 mM, together with 2-mercaptoethanl, up to 50% inhibition | Digitalis lanata | |
Mg2+ | - |
Digitalis lanata | |
Mn2+ | - |
Digitalis lanata | |
NADPH | inhibitory at concentrations above 1 mM | Digitalis lanata | |
Zn2+ | - |
Digitalis lanata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0185 | 0.02 | 5alpha-pregnan-3,20-dione | pH 8.0, 25°C | Digitalis lanata | |
0.05 | 0.12 | NADPH | pH 8.0, 25°C | Digitalis lanata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | major part | Digitalis lanata | 5829 | - |
endoplasmic reticulum | minor part | Digitalis lanata | 5783 | - |
microsome | - |
Digitalis lanata | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-pregnan-3,20-dione + NADPH | Digitalis lanata | - |
3beta-hydroxy-5alpha-pregnane-20-one + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Digitalis lanata | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Digitalis lanata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-pregnan-3,20-dione + NAD(P)H | - |
Digitalis lanata | 3beta-hydroxy-5alpha-pregnan-20-one + NAD(P)+ | i.e. 5alpha-pregnan-3beta-ol-20-one | r | |
5alpha-pregnan-3,20-dione + NADPH | - |
Digitalis lanata | 3beta-hydroxy-5alpha-pregnane-20-one + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
3beta-hydroxysteroid oxidoreductase | - |
Digitalis lanata |
More | 3beta-hydroxysteroid oxidoreductase is exclusively associated with the 5alpha-progesterone reductase in microsomes | Digitalis lanata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Digitalis lanata |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 35 | half-maximal activity at 5°C and 35°C | Digitalis lanata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Digitalis lanata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Digitalis lanata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Digitalis lanata | |
NADH | - |
Digitalis lanata | |
NADP+ | - |
Digitalis lanata | |
NADPH | required | Digitalis lanata |