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Literature summary for 1.1.1.276 extracted from

  • Fujisawa, H.; Nagata, S.; Misono, H.
    Characterization of short-chain dehydrogenase/reductase homologues of Escherichia coli (YdfG) and Saccharomyces cerevisiae (YMR226C) (2003), Biochim. Biophys. Acta, 1645, 89-94.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli JM109 Escherichia coli
overexpression in Escherichia coli JM109 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information substrate specificity Escherichia coli
additional information
-
additional information substrate specificity Saccharomyces cerevisiae
0.5
-
NADP+ pH 9.0, 30°C Saccharomyces cerevisiae
0.54
-
NADP+ pH 9.0, 30°C Escherichia coli
3
-
L-allo-threonine pH 9.0, 30°C Saccharomyces cerevisiae
29
-
L-allo-threonine pH 9.0, 30°C Escherichia coli
40
-
L-serine pH 9.0, 30°C Escherichia coli
95
-
L-serine pH 9.0, 30°C Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
4 * 27000, recombinant enzyme, SDS-PAGE Escherichia coli
29000
-
4 * 29000, recombinant enzyme, SDS-PAGE Saccharomyces cerevisiae
105000
-
recombinant enzyme, gel filtration Escherichia coli
110000
-
recombinant enzyme, gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine + NADP+ Escherichia coli
-
2-aminomalonate semialdehyde + NADPH
-
?
L-serine + NADP+ Saccharomyces cerevisiae
-
2-aminomalonate semialdehyde + NADPH
-
?
L-serine + NADP+ Saccharomyces cerevisiae YMR226C
-
2-aminomalonate semialdehyde + NADPH
-
?
L-serine + NADP+ Escherichia coli YdfG
-
2-aminomalonate semialdehyde + NADPH
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
YdfG
-
Escherichia coli YdfG
-
YdfG
-
Saccharomyces cerevisiae
-
YMR226C
-
Saccharomyces cerevisiae YMR226C
-
YMR226C
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from overproducing Escherichia coli, to homogeneity Escherichia coli
recombinant from overproducing Escherichia coli, to homogeneity Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
purified recombinant enzyme Escherichia coli
6
-
purified recombinant enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methyl-DL-serine + NADP+ 59% activity compared to L-serine Escherichia coli 2-amino-2-methyl-3-oxopropanoic acid + NADPH
-
?
2-methyl-DL-serine + NADP+ 21% activity compared to L-serine Saccharomyces cerevisiae 2-amino-2-methyl-3-oxopropanoic acid + NADPH
-
?
3-hydroxypropanoate + NADP+ 23% activity compared to L-serine Escherichia coli 3-oxopropanoate + NADPH
-
?
3-hydroxypropanoate + NADP+ 13% activity compared to L-serine Saccharomyces cerevisiae 3-oxopropanoate + NADPH
-
?
D-3-hydroxybutyrate + NADP+ 1% activity compared to L-serine Escherichia coli 3-oxobutyrate + NADPH
-
?
D-3-hydroxybutyrate + NADP+ 12% activity compared to L-serine Saccharomyces cerevisiae 3-oxobutyrate + NADPH
-
?
D-3-hydroxyisobutyrate + NADP+ 90% activity compared to L-serine Escherichia coli 2-methyl-3-oxopropanoic acid + NADPH
-
?
D-3-hydroxyisobutyrate + NADP+ 78% activity compared to L-serine Saccharomyces cerevisiae 2-methyl-3-oxopropanoic acid + NADPH
-
?
D-glycerate + NADP+ 48% activity compared to L-serine Escherichia coli ?
-
?
D-glycerate + NADP+ 6% activity compared to L-serine Saccharomyces cerevisiae ?
-
?
D-serine + NADP+ 83% activity compared to L-serine Escherichia coli 2-aminomalonate semialdehyde + NADPH
-
?
D-serine + NADP+ 71% activity compared to L-serine Saccharomyces cerevisiae 2-aminomalonate semialdehyde + NADPH
-
?
D-serine + NADP+ 71% activity compared to L-serine Saccharomyces cerevisiae YMR226C 2-aminomalonate semialdehyde + NADPH
-
?
D-serine + NADP+ 83% activity compared to L-serine Escherichia coli YdfG 2-aminomalonate semialdehyde + NADPH
-
?
D-threonine + NADP+ 156% activity compared to L-serine Escherichia coli D-2-amino-3-oxobutanoic acid + NADPH
-
?
D-threonine + NADP+ 478% activity compared to L-serine Saccharomyces cerevisiae D-2-amino-3-oxobutanoic acid + NADPH
-
?
L-3-hydroxybutyrate + NADP+ 85% activity compared to L-serine Saccharomyces cerevisiae 3-oxobutyrate + NADPH
-
?
L-3-hydroxybutyrate + NADP+ 9% activity compared to L-serine Escherichia coli 3-oxobutyrate + NADPH
-
?
L-3-hydroxyisobutyrate + NADP+ 96% activity compared to L-serine Escherichia coli 2-methyl-3-oxopropanoic acid + NADPH
-
?
L-3-hydroxyisobutyrate + NADP+ 182% activity compared to L-serine Saccharomyces cerevisiae 2-methyl-3-oxopropanoic acid + NADPH
-
?
L-allo-threonine + NADP+ best substrate Escherichia coli L-2-amino-3-ketobutyrate + NADPH L-2-amino-3-ketobutyrate spontaneously decarboxylates into aminoacetone ?
L-allo-threonine + NADP+ best substrate Saccharomyces cerevisiae L-2-amino-3-ketobutyrate + NADPH L-2-amino-3-ketobutyrate spontaneously decarboxylates into aminoacetone ?
L-allo-threonine + NADP+ 137% activity compared to L-serine Escherichia coli L-2-amino-3-ketobutyrate + NADPH L-2-amino-3-ketobutyrate spontaneously decarboxylates into aminoacetone ?
L-allo-threonine + NADP+ 397% activity compared to L-serine Saccharomyces cerevisiae L-2-amino-3-ketobutyrate + NADPH L-2-amino-3-ketobutyrate spontaneously decarboxylates into aminoacetone ?
L-glycerate + NADP+ 28% activity compared to L-serine Escherichia coli ?
-
?
L-glycerate + NADP+ 5% activity compared to L-serine Saccharomyces cerevisiae ?
-
?
L-glycerate + NADP+ 5% activity compared to L-serine Saccharomyces cerevisiae YMR226C ?
-
?
L-glycerate + NADP+ 28% activity compared to L-serine Escherichia coli YdfG ?
-
?
L-serine + NADP+
-
Escherichia coli 2-aminomalonate semialdehyde + NADPH
-
?
L-serine + NADP+
-
Saccharomyces cerevisiae 2-aminomalonate semialdehyde + NADPH
-
?
L-serine + NADP+
-
Saccharomyces cerevisiae YMR226C 2-aminomalonate semialdehyde + NADPH
-
?
L-serine + NADP+
-
Escherichia coli YdfG 2-aminomalonate semialdehyde + NADPH
-
?
additional information no activity with DL-homoserine, O-methyl-L-serine, N-acetyl-DL-serine, DL-isoserine, DL-threo-3-phenylserine, malonate, DL-malate, DL-lactate, DL-tartrate, D-gluconate, citrate, isocitrate, glycolate, glycerol Escherichia coli ?
-
?
additional information no activity with DL-homoserine, O-methyl-L-serine, N-acetyl-DL-serine, DL-isoserine, DL-threo-3-phenylserine, malonate, DL-malate, DL-lactate, DL-tartrate, D-gluconate, citrate, isocitrate, glycolate, glycerol Saccharomyces cerevisiae ?
-
?
additional information L-threonine, D-allo-threonine are poor substrates Escherichia coli ?
-
?
additional information L-threonine, D-allo-threonine are poor substrates Saccharomyces cerevisiae ?
-
?
additional information no activity with DL-homoserine, O-methyl-L-serine, N-acetyl-DL-serine, DL-isoserine, DL-threo-3-phenylserine, malonate, DL-malate, DL-lactate, DL-tartrate, D-gluconate, citrate, isocitrate, glycolate, glycerol Saccharomyces cerevisiae YMR226C ?
-
?
additional information L-threonine, D-allo-threonine are poor substrates Saccharomyces cerevisiae YMR226C ?
-
?
additional information no activity with DL-homoserine, O-methyl-L-serine, N-acetyl-DL-serine, DL-isoserine, DL-threo-3-phenylserine, malonate, DL-malate, DL-lactate, DL-tartrate, D-gluconate, citrate, isocitrate, glycolate, glycerol Escherichia coli YdfG ?
-
?
additional information L-threonine, D-allo-threonine are poor substrates Escherichia coli YdfG ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 27000, recombinant enzyme, SDS-PAGE Escherichia coli
tetramer 4 * 29000, recombinant enzyme, SDS-PAGE Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
10 min, 0.1 M potassium phosphate, pH 7.4, 0.01% 2-mercaptoethanol, 10% glycerol, stable Saccharomyces cerevisiae
55
-
10 min, 0.1 M potassium phosphate, pH 7.4, 0.01% 2-mercaptoethanol, 10% glycerol, stable Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information substrate specificity Escherichia coli
additional information
-
additional information substrate specificity Saccharomyces cerevisiae
4.2
-
L-serine pH 9.0, 30°C Escherichia coli
5.76
-
L-allo-threonine pH 9.0, 30°C Escherichia coli
11
-
L-serine pH 9.0, 30°C Saccharomyces cerevisiae
43.8
-
L-allo-threonine pH 9.0, 30°C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
oxidation of L-serine Escherichia coli
8.5
-
oxidation of L-serine Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 10 10 min, 40°C, stable Escherichia coli
7.5 10.5 10 min, 40°C, stable Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent on Escherichia coli
NADP+ dependent on Saccharomyces cerevisiae
NADP+ NAD+ cannot replace NADP+ Escherichia coli
NADP+ NAD+ cannot replace NADP+ Saccharomyces cerevisiae