BRENDA - Enzyme Database
show all sequences of 1.1.1.276

A novel NADP+ dependent serine dehydrogenase from Agrobacterium tumefaciens

Chowdhury, E.K.; Higuchi, K.; Nagata, S.; Misono, H.; Biosci. Biotechnol. Biochem. 61, 152-157 (1997)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-methylmalonate
competitive against L-serine, noncompetitive against NADP+
Agrobacterium tumefaciens
Co2+
weak
Agrobacterium tumefaciens
D-cysteine
competitive against L-serine, noncompetitive against NADP+
Agrobacterium tumefaciens
Hg2+
strong
Agrobacterium tumefaciens
L-cysteine
competitive against L-serine, noncompetitive against NADP+
Agrobacterium tumefaciens
malonate
competitive against L-serine, noncompetitive against NADP+
Agrobacterium tumefaciens
additional information
no inhibition by EDTA, sodium arsenate, o-phenethroline, alpha,alpha'-dipyridyl and very slight inhibition by sodium azide
Agrobacterium tumefaciens
N-ethylmaleimide
-
Agrobacterium tumefaciens
p-chloromercuribenzoate
strong
Agrobacterium tumefaciens
Tartronate
competitive against L-serine, noncompetitive against NADP+
Agrobacterium tumefaciens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.029
-
NADP+
pH 9.0, 30°C
Agrobacterium tumefaciens
42
-
L-serine
pH 9.0, 30 °C
Agrobacterium tumefaciens
44
-
D-serine
pH 9.0, 30 °C
Agrobacterium tumefaciens
54
-
L-Glycerate
pH 9.0, 30 °C
Agrobacterium tumefaciens
56
-
D-glycerate
pH 9.0, 30 °C
Agrobacterium tumefaciens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no effect with Cu2+, Mg2+, Mn2+, Zn2+, Fe2+
Agrobacterium tumefaciens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
100000
-
gel filtration
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-serine + NADP+
Agrobacterium tumefaciens
-
2-aminomalonate semialdehyde + NADPH
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Agrobacterium tumefaciens
-
-
-
Purification (Commentary)
Commentary
Organism
-
Agrobacterium tumefaciens
Reaction
Reaction
Commentary
Organism
L-serine + NADP+ = 2-aminoacetaldehyde + CO2 + NADPH + H+
sequential mechanism
Agrobacterium tumefaciens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
45.3
-
purified enzyme
Agrobacterium tumefaciens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-methyl-DL-serine + NADP+
8.7% activity compared to L-serine
639082
Agrobacterium tumefaciens
2-amino-2-methyl-3-oxopropanoic acid + NADPH
-
-
-
?
3-hydroxypropanoate + NADP+
1.6% activity compared to L-serine
639082
Agrobacterium tumefaciens
3-oxopropanoate + NADPH
-
-
-
?
D-3-hydroxyisobutyrate + NADP+
3.5% activity compared to L-serine
639082
Agrobacterium tumefaciens
2-methyl-3-oxopropanoic acid + NADPH
-
-
-
?
D-glycerate + NADP+
12.9% activity compared to L-serine
639082
Agrobacterium tumefaciens
?
-
-
-
?
D-serine + NADP+
55.2% activity compared to L-serine
639082
Agrobacterium tumefaciens
2-aminomalonate semialdehyde + NADPH
-
-
-
?
L-3-hydroxyisobutyrate + NADP+
8.5% activity compared to L-serine
639082
Agrobacterium tumefaciens
2-methyl-3-oxopropanoic acid + NADPH
-
-
-
?
L-glycerate + NADP+
11.3% activity compared to L-serine
639082
Agrobacterium tumefaciens
?
-
-
-
?
L-serine + NADP+
-
639082
Agrobacterium tumefaciens
2-aminomalonate semialdehyde + NADPH
-
-
-
?
L-serine + NADP+
best substrate
639082
Agrobacterium tumefaciens
2-aminomalonate semialdehyde + NADPH
-
-
-
?
additional information
no activity with 4-hydroxybutyrate, DL-2-hydroxybutyrate, DL-2-hydroxyisobutyrate, DL-2-hydroxycaproate, DL-2-hydroxyisocaproate, 3-phosphoglycerate, DL-lactate, L-malate, glycollate, glycerol, D-threonine, DL-threo-phenylserine, DL-homoserine, DL-isoserine, L-alanine, beta-alanine, L-aspartate, L-glutamate, L-valine, L-leucine, L-methionine, L-lysine, L-phenylalanine, and glycine
639082
Agrobacterium tumefaciens
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Agrobacterium tumefaciens
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
stable at
Agrobacterium tumefaciens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.1
-
-
Agrobacterium tumefaciens
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7
8
stable at
Agrobacterium tumefaciens
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
dependent on; NAD+ cannot replace NADP+
Agrobacterium tumefaciens
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.067
-
L-cysteine
versus L-serine, pH 9.0, 30°C
Agrobacterium tumefaciens
0.21
-
L-cysteine
versus NADP+, pH 9.0, 30°C
Agrobacterium tumefaciens
0.59
-
D-cysteine
versus L-serine, pH 9.0, 30°C
Agrobacterium tumefaciens
9.9
-
D-cysteine
versus NADP+, pH 9.0, 30°C
Agrobacterium tumefaciens
10
-
2-methylmalonate
versus NADP+, pH 9.0, 30°C
Agrobacterium tumefaciens
11
-
malonate
versus L-serine, pH 9.0, 30°C
Agrobacterium tumefaciens
12
-
2-methylmalonate
versus L-serine, pH 9.0, 30°C
Agrobacterium tumefaciens
13
-
Tartronate
versus L-serine, pH 9.0, 30°C
Agrobacterium tumefaciens
17
-
malonate
versus NADP+, pH 9.0, 30°C
Agrobacterium tumefaciens
18
-
Tartronate
versus NADP+, pH 9.0, 30°C
Agrobacterium tumefaciens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
dependent on; NAD+ cannot replace NADP+
Agrobacterium tumefaciens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-methylmalonate
competitive against L-serine, noncompetitive against NADP+
Agrobacterium tumefaciens
Co2+
weak
Agrobacterium tumefaciens
D-cysteine
competitive against L-serine, noncompetitive against NADP+
Agrobacterium tumefaciens
Hg2+
strong
Agrobacterium tumefaciens
L-cysteine
competitive against L-serine, noncompetitive against NADP+
Agrobacterium tumefaciens
malonate
competitive against L-serine, noncompetitive against NADP+
Agrobacterium tumefaciens
additional information
no inhibition by EDTA, sodium arsenate, o-phenethroline, alpha,alpha'-dipyridyl and very slight inhibition by sodium azide
Agrobacterium tumefaciens
N-ethylmaleimide
-
Agrobacterium tumefaciens
p-chloromercuribenzoate
strong
Agrobacterium tumefaciens
Tartronate
competitive against L-serine, noncompetitive against NADP+
Agrobacterium tumefaciens
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.067
-
L-cysteine
versus L-serine, pH 9.0, 30°C
Agrobacterium tumefaciens
0.21
-
L-cysteine
versus NADP+, pH 9.0, 30°C
Agrobacterium tumefaciens
0.59
-
D-cysteine
versus L-serine, pH 9.0, 30°C
Agrobacterium tumefaciens
9.9
-
D-cysteine
versus NADP+, pH 9.0, 30°C
Agrobacterium tumefaciens
10
-
2-methylmalonate
versus NADP+, pH 9.0, 30°C
Agrobacterium tumefaciens
11
-
malonate
versus L-serine, pH 9.0, 30°C
Agrobacterium tumefaciens
12
-
2-methylmalonate
versus L-serine, pH 9.0, 30°C
Agrobacterium tumefaciens
13
-
Tartronate
versus L-serine, pH 9.0, 30°C
Agrobacterium tumefaciens
17
-
malonate
versus NADP+, pH 9.0, 30°C
Agrobacterium tumefaciens
18
-
Tartronate
versus NADP+, pH 9.0, 30°C
Agrobacterium tumefaciens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.029
-
NADP+
pH 9.0, 30°C
Agrobacterium tumefaciens
42
-
L-serine
pH 9.0, 30 °C
Agrobacterium tumefaciens
44
-
D-serine
pH 9.0, 30 °C
Agrobacterium tumefaciens
54
-
L-Glycerate
pH 9.0, 30 °C
Agrobacterium tumefaciens
56
-
D-glycerate
pH 9.0, 30 °C
Agrobacterium tumefaciens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no effect with Cu2+, Mg2+, Mn2+, Zn2+, Fe2+
Agrobacterium tumefaciens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
100000
-
gel filtration
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-serine + NADP+
Agrobacterium tumefaciens
-
2-aminomalonate semialdehyde + NADPH
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Agrobacterium tumefaciens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
45.3
-
purified enzyme
Agrobacterium tumefaciens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-methyl-DL-serine + NADP+
8.7% activity compared to L-serine
639082
Agrobacterium tumefaciens
2-amino-2-methyl-3-oxopropanoic acid + NADPH
-
-
-
?
3-hydroxypropanoate + NADP+
1.6% activity compared to L-serine
639082
Agrobacterium tumefaciens
3-oxopropanoate + NADPH
-
-
-
?
D-3-hydroxyisobutyrate + NADP+
3.5% activity compared to L-serine
639082
Agrobacterium tumefaciens
2-methyl-3-oxopropanoic acid + NADPH
-
-
-
?
D-glycerate + NADP+
12.9% activity compared to L-serine
639082
Agrobacterium tumefaciens
?
-
-
-
?
D-serine + NADP+
55.2% activity compared to L-serine
639082
Agrobacterium tumefaciens
2-aminomalonate semialdehyde + NADPH
-
-
-
?
L-3-hydroxyisobutyrate + NADP+
8.5% activity compared to L-serine
639082
Agrobacterium tumefaciens
2-methyl-3-oxopropanoic acid + NADPH
-
-
-
?
L-glycerate + NADP+
11.3% activity compared to L-serine
639082
Agrobacterium tumefaciens
?
-
-
-
?
L-serine + NADP+
-
639082
Agrobacterium tumefaciens
2-aminomalonate semialdehyde + NADPH
-
-
-
?
L-serine + NADP+
best substrate
639082
Agrobacterium tumefaciens
2-aminomalonate semialdehyde + NADPH
-
-
-
?
additional information
no activity with 4-hydroxybutyrate, DL-2-hydroxybutyrate, DL-2-hydroxyisobutyrate, DL-2-hydroxycaproate, DL-2-hydroxyisocaproate, 3-phosphoglycerate, DL-lactate, L-malate, glycollate, glycerol, D-threonine, DL-threo-phenylserine, DL-homoserine, DL-isoserine, L-alanine, beta-alanine, L-aspartate, L-glutamate, L-valine, L-leucine, L-methionine, L-lysine, L-phenylalanine, and glycine
639082
Agrobacterium tumefaciens
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Agrobacterium tumefaciens
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
stable at
Agrobacterium tumefaciens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.1
-
-
Agrobacterium tumefaciens
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7
8
stable at
Agrobacterium tumefaciens
Other publictions for EC 1.1.1.276
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
639084
Fujisawa
Characterization of short-chai ...
Escherichia coli, Escherichia coli YdfG, Saccharomyces cerevisiae, Saccharomyces cerevisiae YMR226C
Biochim. Biophys. Acta
1645
89-94
2003
-
-
2
-
-
-
-
8
-
-
4
4
-
4
-
-
2
-
-
-
2
-
40
2
-
-
2
6
2
-
2
2
-
-
-
-
-
2
2
-
-
-
-
-
-
8
-
-
4
4
-
-
-
2
-
-
2
-
40
2
-
-
2
6
2
-
2
-
-
-
-
-
-
-
639083
Fujisawa
Cloning and sequencing of the ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens ICR 1600, Escherichia coli, Escherichia coli JM109
Biosci. Biotechnol. Biochem.
66
1137-1139
2002
-
-
1
-
-
-
-
-
-
-
-
4
-
33
-
-
1
-
-
-
2
-
6
2
2
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
-
-
2
-
6
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
639082
Chowdhury
A novel NADP+ dependent serine ...
Agrobacterium tumefaciens
Biosci. Biotechnol. Biochem.
61
152-157
1997
-
-
-
-
-
-
10
5
-
1
1
1
-
1
-
-
1
1
-
-
1
-
10
-
1
-
1
-
1
-
1
1
10
-
-
-
-
-
1
-
-
-
-
10
10
5
-
1
1
1
-
-
-
1
-
-
1
-
10
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-