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Literature summary for 1.1.1.271 extracted from

  • Ren, Y.; Perepelov, A.V.; Wang, H.; Zhang, H.; Knirel, Y.A.; Wang, L.; Chen, W.
    Biochemical characterization of GDP-L-fucose de novo synthesis pathway in fungus Mortierella alpina (2010), Biochem. Biophys. Res. Commun., 391, 1663-1669.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
DTT 2.1fold activation Mortierella alpina

Cloned(Commentary)

Cloned (Comment) Organism
expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3) Mortierella alpina

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
Mortierella alpina
Cu2+ complete inhibition at 5 mM Mortierella alpina
Fe2+
-
Mortierella alpina
Mn2+
-
Mortierella alpina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetiic modeling Mortierella alpina
1.025
-
NADPH recombinant His-tagged enzyme, pH 8.0, 37°C Mortierella alpina
1.047
-
GDP-4-dehydro-6-deoxy-alpha-D-mannose recombinant His-tagged enzyme, pH 8.0, 37°C Mortierella alpina
1.157
-
NADH recombinant His-tagged enzyme, pH 8.0, 37°C Mortierella alpina

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slight activation at 5 mM Mortierella alpina
Mg2+ slight activation at 5 mM Mortierella alpina
additional information no requirement of divalent cations Mortierella alpina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, recombinant His-tagged enzyme, SDS-PAGE Mortierella alpina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADPH + H+ Mortierella alpina
-
GDP-beta-L-fucose + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Mortierella alpina D3JU54
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Mortierella alpina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADH + H+ the enzyme synthesizes GDP-L-fucose from its substrate GDP-4-keto-6-deoxy-D-mannose. The reaction involves epimerization at both C-3 and C-5 followed by an NADPH-dependent reduction of the carbonyl at C-4 Mortierella alpina GDP-beta-L-fucose + NAD+ product identification by capillary electrophoresis, electro-spray ionization-mass spectrometry, and nuclear magnetic resonance spectroscopy ?
GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADPH + H+
-
Mortierella alpina GDP-beta-L-fucose + NADP+
-
?
GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADPH + H+ the enzyme synthesizes GDP-L-fucose from its substrate GDP-4-keto-6-deoxy-D-mannose. The reaction involves epimerization at both C-3 and C-5 followed by an NADPH-dependent reduction of the carbonyl at C-4 Mortierella alpina GDP-beta-L-fucose + NADP+ product identification by capillary electrophoresis, electro-spray ionization-mass spectrometry, and nuclear magnetic resonance spectroscopy ?

Subunits

Subunits Comment Organism
? x * 40000, recombinant His-tagged enzyme, SDS-PAGE Mortierella alpina

Synonyms

Synonyms Comment Organism
GDP-keto-6-deoxymannose 3,5-epimerase/4-reductase
-
Mortierella alpina
GMER
-
Mortierella alpina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
recombinant His-tagged enzyme Mortierella alpina

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 55 activity range Mortierella alpina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
recombinant His-tagged enzyme Mortierella alpina

pH Range

pH Minimum pH Maximum Comment Organism
5 11 activity range Mortierella alpina

Cofactor

Cofactor Comment Organism Structure
additional information both NADH and NADPH may be used as the cofactor by the enzyme, which shows similar affinities to NADH and NADPH, but the Vmax value is higher for NADPH Mortierella alpina
NADH
-
Mortierella alpina
NADPH
-
Mortierella alpina