Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP | competitive with respect to GDP-4-keto-6-deoxymannose | Escherichia coli | |
GDP-fucose | competitive with respect to NADPH and GDP-4-keto-6-deoxymannose | Escherichia coli | |
NADP | competitive inhibitor | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
NADPH | - |
Escherichia coli | |
0.109 | - |
NADH | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADH + H+ | - |
Escherichia coli | GDP-beta-L-fucose + NAD+ | - |
r | |
GDP-4-keto-6-deoxy-D-mannose + NADPH | catalyzes the stereospecific hydride transfer of the ProS hydrogen from NADPH to carbon 4 of the mannose sugar | Escherichia coli | GDP-6-deoxy-L-galactose + NADP+ | - |
? | |
GDP-4-keto-6-deoxy-D-mannose + NADPH | enzyme also utilizes NADH but with lower affinity | Escherichia coli | GDP-6-deoxy-L-galactose + NADP+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | enzyme has lower affinity for NADH as compared with NADPH | Escherichia coli | |
NADPH | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
GDP-fucose | - |
Escherichia coli | |
0.061 | - |
GDP | - |
Escherichia coli | |
0.069 | - |
NADP+ | - |
Escherichia coli |