Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.27 extracted from

  • Lu, H.; Bai, Y.; Fan, T.; Zhao, Y.; Zheng, X.; Cai, Y.
    Identification of a L-Lactate dehydrogenase with 3,4-dihydroxyphenylpyruvic reduction activity for L-danshensu production (2018), Process Biochem., 72, 119-123 .
No PubMed abstract available
Search for more literature for 1.1.1.27

Application

Application Comment Organism
synthesis coexpression of enzyme and glucose dehydrogenase gene in Escherichia coli efficiently reduces 3,4-dihydroxyphenylpyruvate to L-3,4-dihydroxyphenyllactate with 95.45% isolation yield Limosilactobacillus fermentum

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 6 h, 60% decrease in activity Limosilactobacillus fermentum
Fe2+ 6 h, 70% decrease in activity Limosilactobacillus fermentum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.123
-
 NADH pH 6, 25°C Limosilactobacillus fermentum
0.197
-
 NADPH pH 6, 25°C Limosilactobacillus fermentum
2.47
-
 pyruvate pH 6, 25°C Limosilactobacillus fermentum
11.37
-
 3,4-Dihydroxyphenylpyruvate pH 6, 25°C Limosilactobacillus fermentum

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 6 h, 20% increase in activity Limosilactobacillus fermentum

Organism

Organism UniProt Comment Textmining
Limosilactobacillus fermentum A0A2K9Y5T7
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.48
-
 substrate 3,4-dihydroxyphenylpyruvate, pH 6, 25°C Limosilactobacillus fermentum
144.8
-
 substrate pyruvate, pH 6, 25°C Limosilactobacillus fermentum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutyrate + NADH + H+ 11.5% of the activity with pyruvate Limosilactobacillus fermentum 2-hydroxybutyrate + NAD+
-
 ?
2-oxoglutarate + NADH + H+ 14.6% of the activity with pyruvate Limosilactobacillus fermentum 2-hydroxyglutarate + NAD+
-
 ?
3,4-dihydroxyphenylpyruvate + NADH + H+ 8.5% of the activity with pyruvate Limosilactobacillus fermentum L-3,4-dihydroxyphenyllactate + NAD+
-
 ?
4-hydroxyphenylpyruvate + NADH + H+ 11.1% of the activity with pyruvate Limosilactobacillus fermentum L-4-hydroxyphenyllactate + NAD+
-
 ?
4-methyl-2-oxopentanoate + NADH + H+ 25.9% of the activity with pyruvate Limosilactobacillus fermentum 2-hydroxy-4-methylpentanoate + NAD+
-
 ?
benzoylformate + NADH + H+ 20.5% of the activity with pyruvate Limosilactobacillus fermentum L-hydroxy(phenyl)acetate + NAD+
-
 ?
phenylpyruvate + NADH + H+ 47.4% of the activity with pyruvate Limosilactobacillus fermentum L-phenyllactate + NAD+
-
 ?
pyruvate + NADPH + H+
-
 Limosilactobacillus fermentum L-lactate + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 33650, calcuated from sequence Limosilactobacillus fermentum

Synonyms

Synonyms Comment Organism
L-Ldh0845
-
 Limosilactobacillus fermentum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Limosilactobacillus fermentum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.293
-
 3,4-Dihydroxyphenylpyruvate pH 6, 25°C Limosilactobacillus fermentum
10.37
-
 NADPH pH 6, 25°C Limosilactobacillus fermentum
12.45
-
 pyruvate pH 6, 25°C Limosilactobacillus fermentum
64.42
-
 NADH pH 6, 25°C Limosilactobacillus fermentum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Limosilactobacillus fermentum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 stable Limosilactobacillus fermentum

Cofactor

Cofactor Comment Organism Structure
NADH NADH is 10fold preferred over NADPH Limosilactobacillus fermentum
NADPH NADH is 10fold preferred over NADPH Limosilactobacillus fermentum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0258
-
 3,4-Dihydroxyphenylpyruvate pH 6, 25°C Limosilactobacillus fermentum
5.05
-
 pyruvate pH 6, 25°C Limosilactobacillus fermentum
52.52
-
 NADPH pH 6, 25°C Limosilactobacillus fermentum
521.9
-
 NADH pH 6, 25°C Limosilactobacillus fermentum