Protein Variants | Comment | Organism |
---|---|---|
I229A | 30% decrease in Km value and 2.6fold increase in kcat value for phenylpyruvate | Lacticaseibacillus casei |
Q88R | 17% decrease in Km value and 3fold increase in kcat value for phenylpyruvate | Lacticaseibacillus casei |
T235G | slight decrease in specific activity towards phenylpyruvate | Lacticaseibacillus casei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.86 | - |
phenylpyruvate | mutant Q88R, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei | |
2.5 | 5 | phenylpyruvate | mutant I229A, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei | |
3.5 | - |
phenylpyruvate | wild-type, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei | |
5.75 | - |
phenylpyruvate | mutant I229A, pH 5.5, 30°C | Lacticaseibacillus casei | |
6.82 | - |
phenylpyruvate | mutant Q88R, pH 5.5, 30°C | Lacticaseibacillus casei | |
8.23 | - |
phenylpyruvate | wild-type, pH 5.5, 30°C | Lacticaseibacillus casei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lacticaseibacillus casei | A0A2U9AUU1 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.5 | - |
wild-type, substrate benzoylformic acid, pH 5.5, 30°C | Lacticaseibacillus casei |
17.4 | - |
wild-type, substrate 4-hydroxyphenylpyruvate, pH 5.5, 30°C | Lacticaseibacillus casei |
25 | 40 | wild-type, substrate pyruvate, pH 5.5, 30°C | Lacticaseibacillus casei |
66.7 | - |
mutant T235G, substrate phenylpyruvate, pH 5.5, 30°C | Lacticaseibacillus casei |
72.3 | - |
wild-type, substrate phenylpyruvate, pH 5.5, 30°C | Lacticaseibacillus casei |
168.4 | - |
wild-type, substrate 2-oxobutanoate, pH 5.5, 30°C | Lacticaseibacillus casei |
435.8 | - |
mutant I229A, substrate phenylpyruvate, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei |
451.5 | - |
mutant Q88R, substrate phenylpyruvate, pH 5.5, 30°C | Lacticaseibacillus casei |
512.4 | - |
mutant I229A, substrate phenylpyruvate, pH 5.5, 30°C | Lacticaseibacillus casei |
671.2 | - |
wild-type, substrate oxaloacetic acid, pH 5.5, 30°C | Lacticaseibacillus casei |
680.3 | - |
mutant T235G, substrate phenylpyruvate, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei |
798 | - |
wild-type, substrate phenylpyruvate, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei |
4071 | - |
mutant Q88R, substrate phenylpyruvate, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + NADH + H+ | - |
Lacticaseibacillus casei | 2-hydroxybutanoate + NAD+ | - |
? | |
4-hydroxyphenylpyruvate + NADH + H+ | - |
Lacticaseibacillus casei | L-4-hydroxyphenyllactate + NAD+ | - |
? | |
benzoylformic acid + NADH + H+ | - |
Lacticaseibacillus casei | ? + NAD+ | - |
? | |
oxaloacetate + NADH + H+ | - |
Lacticaseibacillus casei | ? + NAD+ | - |
? | |
phenylpyruvate + NADH + H+ | - |
Lacticaseibacillus casei | L-phenyllactate + NAD+ | - |
? | |
pyruvate + NADH + H+ | - |
Lacticaseibacillus casei | L-lactate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36800, SDS-PAGE | Lacticaseibacillus casei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
94.7 | - |
phenylpyruvate | wild-type, pH 5.5, 30°C | Lacticaseibacillus casei | |
344.4 | - |
phenylpyruvate | mutant I229A, pH 5.5, 30°C | Lacticaseibacillus casei | |
380.4 | - |
phenylpyruvate | mutant Q88R, pH 5.5, 30°C | Lacticaseibacillus casei | |
1293 | - |
phenylpyruvate | mutant I229A, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei | |
6467 | - |
phenylpyruvate | wild-type, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei | |
17479 | - |
phenylpyruvate | mutant Q88R, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.5 | - |
phenylpyruvate | wild-type, pH 5.5, 30°C | Lacticaseibacillus casei | |
55.8 | - |
phenylpyruvate | mutant Q88R, pH 5.5, 30°C | Lacticaseibacillus casei | |
59.9 | - |
phenylpyruvate | mutant I229A, pH 5.5, 30°C | Lacticaseibacillus casei | |
507 | - |
phenylpyruvate | mutant I229A, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei | |
1848 | - |
phenylpyruvate | wild-type, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei | |
9397 | - |
phenylpyruvate | mutant Q88R, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C | Lacticaseibacillus casei |