Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pyruvate | at high concentrations | Saduria entomon |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
pyruvate | at 0°C and at 5°C | Saduria entomon | |
0.12 | - |
pyruvate | 10°C | Saduria entomon | |
0.13 | - |
pyruvate | 15°C | Saduria entomon | |
0.14 | - |
pyruvate | 20°C | Saduria entomon | |
0.15 | - |
pyruvate | 25°C | Saduria entomon | |
0.18 | - |
pyruvate | 30°C | Saduria entomon | |
90.04 | - |
(S)-lactate | - |
Saduria entomon |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
4 * 36000, SDS-PAGE | Saduria entomon |
140000 | - |
gel filtration | Saduria entomon |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saduria entomon | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saduria entomon |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pectoral muscle | - |
Saduria entomon | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
510 | - |
- |
Saduria entomon |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD+ | - |
Saduria entomon | pyruvate + NADH + H+ | - |
r | |
pyruvate + NADH + H+ | - |
Saduria entomon | (S)-lactate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 36000, SDS-PAGE | Saduria entomon |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
20 min, stable up to | Saduria entomon |
71.5 | - |
20 min, 50% loss of activity | Saduria entomon |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
reduction of pyruvate | Saduria entomon |
9.1 | - |
oxidation of lactate | Saduria entomon |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | coenzyme | Saduria entomon | |
NADH | coenzyme | Saduria entomon |