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Literature summary for 1.1.1.27 extracted from

  • Hensel, R.; Mayr, U.; Stetter, K.O.; Kandler, O.
    Comparative studies of lactic acid dehydrogenases in lactic acid bacteria. I. Purification and kinetics of the allosteric L-lactic acid dehydrogenase from Lactobacillus casei ssp. casei and Lactobacillus curvatus (1977), Arch. Microbiol., 112, 81-93.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
fructose 1,6-diphosphate L-lactate dehydrogenase form which is activated by fructose 1,6-diphosphate Lacticaseibacillus casei
fructose 1,6-diphosphate L-lactate dehydrogenase form which is activated by fructose 1,6-diphosphate Latilactobacillus curvatus

General Stability

General Stability Organism
irreversible loss of activity after several hours when the concentration is below 0.1 mg protein per ml Latilactobacillus curvatus
Mn2+ and fructose 1,6-diphosphate are required during dialysis at pH 5.5, very unstable during dialysis at pH 7.5 although Mn2+ and fructose 1,6-diphosphate are added Latilactobacillus curvatus
stable to dilution in the range 0.2-0.01 mg protein per ml Lacticaseibacillus casei
very stable during 70 h dialysis in acetate buffer pH 5.5, in imidazol buffer, pH 6.5, in phosphate buffer pH 6.5 with dithioerythritol and in Tris-HCl buffer pH 7.5 with Mn2+ and fructose 1,6-diphosphate Lacticaseibacillus casei

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ at low pH-values Lacticaseibacillus casei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information influence of pH Lacticaseibacillus casei
additional information
-
additional information influence of pH Latilactobacillus curvatus
280
-
(S)-lactate
-
Latilactobacillus curvatus
420
-
(S)-lactate
-
Lacticaseibacillus casei

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulation is as effective as with Mn2+, Co2+ and Cd2+ Lacticaseibacillus casei
Ca2+ 50% of the stimulation with Mn2+, Co2+ or Cd2+, higher concentrations required Latilactobacillus curvatus
Cd2+ stimulation Lacticaseibacillus casei
Cd2+ stimulation Latilactobacillus curvatus
Co2+ stimulation Lacticaseibacillus casei
Co2+ stimulation Latilactobacillus curvatus
Mn2+ stimulation Lacticaseibacillus casei
Mn2+ stimulation Latilactobacillus curvatus

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus casei
-
ssp. casein
-
Latilactobacillus curvatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lacticaseibacillus casei
-
Latilactobacillus curvatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2030
-
-
Latilactobacillus curvatus
2320
-
-
Lacticaseibacillus casei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + NAD+ the maximal velocity of lactate oxidation is about 10% of pyruvate reduction Lacticaseibacillus casei pyruvate + NADH + H+
-
r
(S)-lactate + NAD+ the maximal velocity of lactate oxidation is about 10% of pyruvate reduction Latilactobacillus curvatus pyruvate + NADH + H+
-
r
pyruvate + NADH + H+ the maximal velocity of lactate oxidation is only 10% of pyruvate reduction Lacticaseibacillus casei (S)-lactate + NAD+
-
r
pyruvate + NADH + H+ the maximal velocity of lactate oxidation is only 10% of pyruvate reduction Latilactobacillus curvatus (S)-lactate + NAD+
-
r
pyruvate + NADPH + H+ 20% of the activity with NADH Latilactobacillus curvatus (S)-lactate + NADP+
-
?
pyruvate + NADPH + H+ 15% of the activity with NADH Lacticaseibacillus casei (S)-lactate + NADP+
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
relatively stable Lacticaseibacillus casei
60
-
5 min, complete inactivation without stabilizer, Mn2+ in combination with fructose 1,6-diphosphate stabilizes the enzyme completely Latilactobacillus curvatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Lacticaseibacillus casei
4.5
-
-
Latilactobacillus curvatus

Cofactor

Cofactor Comment Organism Structure
NAD+ coenzyme Lacticaseibacillus casei
NAD+ coenzyme Latilactobacillus curvatus
NADH coenzyme Lacticaseibacillus casei
NADH coenzyme Latilactobacillus curvatus
NADPH coenzyme Lacticaseibacillus casei
NADPH coenzyme Latilactobacillus curvatus