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Literature summary for 1.1.1.267 extracted from

  • Cheng, Z.; Wang, R.; Shen, J.; Yang, Z.; Chen, Y.
    Cloning, characterization, and expression of 1-deoxy-D-xylulose-5-phosphate reductoisomerase gene from Dioscorea zingiberensis (2019), Biol. Plant., 63, 262-267 .
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Cloned(Commentary)

Cloned (Comment) Organism
gene dxr, DNA and amino acid sequence determination and analysis, sequence comparisons with other plant enzymes, quantitative real-time PCR enzyme expression analysis Dioscorea zingiberensis

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast the sequence of DzDXR has a putative plastid transit peptide at the N-terminal region Dioscorea zingiberensis 9507
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Dioscorea zingiberensis
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 2-C-methyl-D-erythritol 4-phosphate + NADP+
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 ?

Organism

Organism UniProt Comment Textmining
Dioscorea zingiberensis A0A1P8JPV2
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Source Tissue

Source Tissue Comment Organism Textmining
leaf young and mature Dioscorea zingiberensis
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additional information during the growth period of Dioscorea zingiberensis, the expression of DzDXR is significantly high in leaves and very low in tubers, and the highest expression is observed in mature leaves in summer, quantitative real-time PCR enzyme expression analysis Dioscorea zingiberensis
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tuber young and old Dioscorea zingiberensis
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+
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 Dioscorea zingiberensis 2-C-methyl-D-erythritol 4-phosphate + NADP+
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 ?

Synonyms

Synonyms Comment Organism
DXR
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 Dioscorea zingiberensis
DzDXR
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 Dioscorea zingiberensis

Cofactor

Cofactor Comment Organism Structure
NADP+
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 Dioscorea zingiberensis
NADPH
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 Dioscorea zingiberensis

General Information

General Information Comment Organism
evolution Dioscorea zingiberensis DzDXR is highly homologous to other plant DXRs, especially to those in monocotyledons Dioscorea zingiberensis
metabolism 1-deoxy-D-xylulose 5-phosphate-reductoisomerase (DXR,) is the key enzyme in the plastidic 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. In contrast to the DzDXR transcription, diosgenin is present predominantly in tubers and in minute quantities in leaves. Because diosgenin is very likely formed mainly in the cytosol of mature leaf cells of Dioscorea zingiberensis and the plastidic IPP and DMAPP produced by the MEP pathway can be transported into the cytosol, the consistently high expression of DzDXR detected in mature leaf of Dioscorea zingiberensis implies that the MEP pathway might play a significant role in diosgenin biosynthesis Dioscorea zingiberensis