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Literature summary for 1.1.1.267 extracted from

  • Haymond, A.; Johny, C.; Dowdy, T.; Schweibenz, B.; Villarroel, K.; Young, R.; Mantooth, C.J.; Patel, T.; Bases, J.; San Jose, G.; Jackson, E.R.; Dowd, C.S.; Couch, R.D.
    Kinetic characterization and allosteric inhibition of the Yersinia pestis 1-deoxy-D-xylulose 5-phosphate reductoisomerase (MEP synthase) (2014), PLoS ONE, 9, e106243.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.267

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Yersinia pestis

Inhibitors

Inhibitors Comment Organism Structure
fosmidomycin
-
 Yersinia pestis
FR900098
-
 Yersinia pestis
[3-[acetyl(naphthalen-2-ylmethoxy)amino]propyl]phosphonic acid compound binds to both the NADPH and DXP sites, while whole-cell inhibitory activity is relatively poor Yersinia pestis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0127
-
 NADPH pH 7.8, 37°C Yersinia pestis
0.222
-
 1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C Yersinia pestis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43100
-
 x * 43100, calculated Yersinia pestis

Organism

Organism UniProt Comment Textmining
Yersinia pestis Q8ZH62
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
 Yersinia pestis 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 43100, calculated Yersinia pestis

Synonyms

Synonyms Comment Organism
IspC
-
 Yersinia pestis
MEP synthase
-
 Yersinia pestis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 NADPH pH 7.8, 37°C Yersinia pestis
1.7
-
 1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C Yersinia pestis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00017
-
 FR900098 pH 7.8, 37°C Yersinia pestis
0.00097
-
 fosmidomycin pH 7.8, 37°C Yersinia pestis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00023
-
 pH 7.8, 37°C Yersinia pestis FR900098
0.00071
-
 pH 7.8, 37°C Yersinia pestis fosmidomycin

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.3
-
 1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C Yersinia pestis