KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.073 | - |
1-deoxy-D-xylulose-5-phosphate | pH 7.7, presence of 1 mM MnCl2 | Escherichia coli | |
0.097 | - |
1-deoxy-D-xylulose-5-phosphate | pH 7.7, presence of 3 mM MgCl2 | Escherichia coli | |
0.158 | - |
methyl-D-erythritol-4-phosphate | pH 7.7, presence of 3 mM MgCl2 | Escherichia coli | |
0.294 | - |
methyl-D-erythritol-4-phosphate | pH 7.7, presence of 1 mM MnCl2 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | or Mn2+, required | Escherichia coli | |
Mn2+ | or Mg2+, required | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
recombinant enzyme, His-tagged | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose-5-phosphate + NADPH | - |
Escherichia coli | methyl-D-erythritol-4-phosphate + NADP+ | - |
r | |
additional information | reaction depends on NADPH although it does not involve a reduction step, putative aldehyde intermediate is converted to methyl-D-erythritol-4-phosphate in the presence of NADPH and to 1-deoxy-D-xylolose-5-phosphate in the presence of NADP+ | Escherichia coli | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli | |
NADPH | - |
Escherichia coli |