Cloned (Comment) | Organism |
---|---|
gene 1029130, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) | Vitis vinifera |
gene 1029130, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) | Vitis californica |
gene 1029130, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) | Ampelopsis glandulosa var. brevipedunculata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
L-Idonate | pH 5.4, 30°C, recombinant enzyme | Vitis vinifera | |
12.5 | - |
5-Dehydro-D-gluconate | pH 8.5, 30°C, recombinant enzyme | Vitis vinifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-idonate + NADP+ | Vitis vinifera | the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview | 5-dehydro-D-gluconate + NADPH | - |
r | |
L-idonate + NADP+ | Vitis californica | the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview | 5-dehydro-D-gluconate + NADPH | - |
r | |
L-idonate + NADP+ | Ampelopsis glandulosa var. brevipedunculata | the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview | 5-dehydro-D-gluconate + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ampelopsis glandulosa var. brevipedunculata | - |
- |
- |
no activity in Ampelopsis aconitifolia | - |
- |
- |
Vitis californica | - |
- |
- |
Vitis vinifera | Q1PSI9 | cv. Cabernet Sauvignon | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) | Vitis vinifera |
recombinant enzyme from Escherichia coli strain BL21(DE3) | Vitis californica |
recombinant enzyme from Escherichia coli strain BL21(DE3) | Ampelopsis glandulosa var. brevipedunculata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Vitis vinifera | - |
fruit | - |
Vitis californica | - |
fruit | - |
Ampelopsis glandulosa var. brevipedunculata | - |
leaf | young | Vitis vinifera | - |
leaf | young | Vitis californica | - |
leaf | young | Ampelopsis glandulosa var. brevipedunculata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
in vivo activity, feeding experiments, overview | Vitis vinifera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-idonate + NADP+ | - |
Vitis vinifera | 5-dehydro-D-gluconate + NADPH | i.e. 5-keto-D-gluconic acid | r | |
L-idonate + NADP+ | - |
Vitis californica | 5-dehydro-D-gluconate + NADPH | i.e. 5-keto-D-gluconic acid | r | |
L-idonate + NADP+ | - |
Ampelopsis glandulosa var. brevipedunculata | 5-dehydro-D-gluconate + NADPH | i.e. 5-keto-D-gluconic acid | r | |
L-idonate + NADP+ | the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview | Vitis vinifera | 5-dehydro-D-gluconate + NADPH | - |
r | |
L-idonate + NADP+ | the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview | Vitis californica | 5-dehydro-D-gluconate + NADPH | - |
r | |
L-idonate + NADP+ | the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview | Ampelopsis glandulosa var. brevipedunculata | 5-dehydro-D-gluconate + NADPH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
L-IdnDH | - |
Vitis vinifera |
L-IdnDH | - |
Vitis californica |
L-IdnDH | - |
Ampelopsis glandulosa var. brevipedunculata |
L-idonate dehydrogenase | - |
Vitis vinifera |
L-idonate dehydrogenase | - |
Vitis californica |
L-idonate dehydrogenase | - |
Ampelopsis glandulosa var. brevipedunculata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Vitis vinifera |
30 | - |
assay at | Vitis californica |
30 | - |
assay at | Ampelopsis glandulosa var. brevipedunculata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
forward, oxidation reaction | Vitis vinifera |
5.4 | - |
forward, oxidation reaction | Vitis californica |
5.4 | - |
forward, oxidation reaction | Ampelopsis glandulosa var. brevipedunculata |
8.5 | - |
reverse, reduction reaction | Vitis vinifera |
8.5 | - |
reverse, reduction reaction | Vitis californica |
8.5 | - |
reverse, reduction reaction | Ampelopsis glandulosa var. brevipedunculata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Vitis vinifera | |
NADP+ | - |
Vitis californica | |
NADP+ | - |
Ampelopsis glandulosa var. brevipedunculata | |
NADPH | - |
Vitis vinifera | |
NADPH | - |
Vitis californica | |
NADPH | - |
Ampelopsis glandulosa var. brevipedunculata |