Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Aeropyrum pernix |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
K+ | slight inhibition at 70 mM | Aeropyrum pernix | |
Na+ | slight inhibition at 70 mM | Aeropyrum pernix | |
NAD(P)+ | competitive with NAD(P)H and noncompetitive with dihydroxyacetone phosphate | Aeropyrum pernix | |
sn-glycerol-1-phosphate | noncompetitive with dihydroxyacetone phosphate and NAD(P)H | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
NADH | pH 7.0, 65°C | Aeropyrum pernix | |
0.044 | - |
NADPH | pH 7.0, 65°C | Aeropyrum pernix | |
0.29 | - |
dihydroxyacetone phosphate | in the presence of 0.32 mM NADPH, pH 7.0, 65°C | Aeropyrum pernix | |
0.46 | - |
dihydroxyacetone phosphate | in the presence of 0.32 mM NADH, pH 7.0, 65°C | Aeropyrum pernix | |
1.57 | - |
NAD+ | pH 7.0, 65°C | Aeropyrum pernix | |
8.92 | - |
sn-glycerol-1-phosphate | pH 7.0, 65°C | Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Aeropyrum pernix |
72400 | - |
gel filtration | Aeropyrum pernix |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone phosphate + NAD(P)H | Aeropyrum pernix | key enzyme in the formation of archeal enantiomeric polar lipid structures | sn-glycerol-1-phosphate + NAD(P)+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | - |
hyperthermophilic archeon | - |
Purification (Comment) | Organism |
---|---|
recombinant protein | Aeropyrum pernix |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
sn-glycerol 1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H + H+ | ordered bi-bi mechanism | Aeropyrum pernix |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.22 | - |
purified enzyme, pH 7.0, 65°C | Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone phosphate + NAD(P)H | key enzyme in the formation of archeal enantiomeric polar lipid structures | Aeropyrum pernix | sn-glycerol-1-phosphate + NAD(P)+ | - |
r | |
dihydroxyacetone phosphate + NAD(P)H | reverse reaction only with NAD+ as cofactor | Aeropyrum pernix | sn-glycerol-1-phosphate + NAD(P)+ | - |
r | |
additional information | no activity with glycerol-2-phosphate, glycerol-3-phosphate, glyceraldehyde phosphate, glycerol, and dihydroxyacetone | Aeropyrum pernix | ? | - |
? | |
sn-glycerol-1-phosphate + NAD+ | no reaction with NADP+ | Aeropyrum pernix | dihydroxyacetone phosphate + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, SDS-PAGE | Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
94 | 96 | - |
Aeropyrum pernix |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 96 | - |
Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life: 2 h | Aeropyrum pernix |
95 | - |
half-life: 0.5 h | Aeropyrum pernix |
96 | - |
unstable above 96°C | Aeropyrum pernix |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.044 | - |
NADPH | pH 7.0, 65°C | Aeropyrum pernix | |
0.09 | - |
NAD+ | pH 7.0, 65°C | Aeropyrum pernix | |
0.09 | - |
sn-glycerol-1-phosphate | pH 7.0, 65°C | Aeropyrum pernix | |
0.75 | - |
dihydroxyacetone phosphate | in the presence of 0.32 mM NADPH, pH 7.0, 65°C | Aeropyrum pernix | |
2.4 | - |
NADH | pH 7.0, 65°C | Aeropyrum pernix | |
2.6 | - |
dihydroxyacetone phosphate | in the presence of 0.32 mM NADH, pH 7.0, 65°C | Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Aeropyrum pernix |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Aeropyrum pernix | |
NADH | - |
Aeropyrum pernix | |
NADP+ | no activity with sn-glycerol-1-phosphate as substrate | Aeropyrum pernix | |
NADPH | - |
Aeropyrum pernix |