Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.261 extracted from

  • Han, J.S.; Kosugi, Y.; Ishida, H.; Ishikawa, K.
    Kinetic study of sn-glycerol-1-phosphate dehydrogenase from the aerobic hyperthermophilic archaeon, Aeropyrum pernix K1 (2002), Eur. J. Biochem., 269, 969-976.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Aeropyrum pernix

Inhibitors

Inhibitors Comment Organism Structure
K+ slight inhibition at 70 mM Aeropyrum pernix
Na+ slight inhibition at 70 mM Aeropyrum pernix
NAD(P)+ competitive with NAD(P)H and noncompetitive with dihydroxyacetone phosphate Aeropyrum pernix
sn-glycerol-1-phosphate noncompetitive with dihydroxyacetone phosphate and NAD(P)H Aeropyrum pernix

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
NADH pH 7.0, 65°C Aeropyrum pernix
0.044
-
NADPH pH 7.0, 65°C Aeropyrum pernix
0.29
-
dihydroxyacetone phosphate in the presence of 0.32 mM NADPH, pH 7.0, 65°C Aeropyrum pernix
0.46
-
dihydroxyacetone phosphate in the presence of 0.32 mM NADH, pH 7.0, 65°C Aeropyrum pernix
1.57
-
NAD+ pH 7.0, 65°C Aeropyrum pernix
8.92
-
sn-glycerol-1-phosphate pH 7.0, 65°C Aeropyrum pernix

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
2 * 38000, SDS-PAGE Aeropyrum pernix
72400
-
gel filtration Aeropyrum pernix

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydroxyacetone phosphate + NAD(P)H Aeropyrum pernix key enzyme in the formation of archeal enantiomeric polar lipid structures sn-glycerol-1-phosphate + NAD(P)+
-
r

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix
-
hyperthermophilic archeon
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Aeropyrum pernix

Reaction

Reaction Comment Organism Reaction ID
sn-glycerol 1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H + H+ ordered bi-bi mechanism Aeropyrum pernix

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.22
-
purified enzyme, pH 7.0, 65°C Aeropyrum pernix

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroxyacetone phosphate + NAD(P)H key enzyme in the formation of archeal enantiomeric polar lipid structures Aeropyrum pernix sn-glycerol-1-phosphate + NAD(P)+
-
r
dihydroxyacetone phosphate + NAD(P)H reverse reaction only with NAD+ as cofactor Aeropyrum pernix sn-glycerol-1-phosphate + NAD(P)+
-
r
additional information no activity with glycerol-2-phosphate, glycerol-3-phosphate, glyceraldehyde phosphate, glycerol, and dihydroxyacetone Aeropyrum pernix ?
-
?
sn-glycerol-1-phosphate + NAD+ no reaction with NADP+ Aeropyrum pernix dihydroxyacetone phosphate + NADH
-
r

Subunits

Subunits Comment Organism
dimer 2 * 38000, SDS-PAGE Aeropyrum pernix

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
94 96
-
Aeropyrum pernix

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 96
-
Aeropyrum pernix

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
half-life: 2 h Aeropyrum pernix
95
-
half-life: 0.5 h Aeropyrum pernix
96
-
unstable above 96°C Aeropyrum pernix

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.044
-
NADPH pH 7.0, 65°C Aeropyrum pernix
0.09
-
NAD+ pH 7.0, 65°C Aeropyrum pernix
0.09
-
sn-glycerol-1-phosphate pH 7.0, 65°C Aeropyrum pernix
0.75
-
dihydroxyacetone phosphate in the presence of 0.32 mM NADPH, pH 7.0, 65°C Aeropyrum pernix
2.4
-
NADH pH 7.0, 65°C Aeropyrum pernix
2.6
-
dihydroxyacetone phosphate in the presence of 0.32 mM NADH, pH 7.0, 65°C Aeropyrum pernix

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Aeropyrum pernix

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Aeropyrum pernix
NADH
-
Aeropyrum pernix
NADP+ no activity with sn-glycerol-1-phosphate as substrate Aeropyrum pernix
NADPH
-
Aeropyrum pernix