Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | slight inhibition at 1 mM | Methanothermobacter thermautotrophicus | |
Cu2+ | slight inhibition at 1 mM | Methanothermobacter thermautotrophicus | |
DTT | 45% inhibition at 1 mM | Methanothermobacter thermautotrophicus | |
Ni2+ | slight inhibition at 1 mM | Methanothermobacter thermautotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
NADPH | dihydroxyacetone phosphate reduction | Methanothermobacter thermautotrophicus | |
0.127 | - |
NAD+ | glycerol-1-phosphate oxidation | Methanothermobacter thermautotrophicus | |
0.129 | - |
NADH | dihydroxyacetone phosphate reduction | Methanothermobacter thermautotrophicus | |
0.27 | - |
NADP+ | glycerol-1-phosphate oxidation | Methanothermobacter thermautotrophicus | |
0.58 | - |
dihydroxyacetone phosphate | in the presence of NADPH | Methanothermobacter thermautotrophicus | |
2.17 | - |
dihydroxyacetone phosphate | - |
Methanothermobacter thermautotrophicus | |
4.8 | - |
glycerol-1-phosphate | in the presence of NADPH | Methanothermobacter thermautotrophicus | |
16.3 | - |
glycerol-1-phosphate | - |
Methanothermobacter thermautotrophicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | maximum activity at 60-80 mM | Methanothermobacter thermautotrophicus | |
Na+ | maximum activity 20-25 mM | Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
8 * 38000, SDS-PAGE | Methanothermobacter thermautotrophicus |
302000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone phosphate + NAD(P)H | Methanothermobacter thermautotrophicus | formation of glycerol-1-phosphate is the natural direction of the reaction | sn-glycerol-1-phosphate + NAD(P)+ | - |
r | |
sn-glycerol-1-phosphate + NAD+ | Methanothermobacter thermautotrophicus | - |
dihydroxyacetone phosphate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, 7step chromatography | Methanothermobacter thermautotrophicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Methanothermobacter thermautotrophicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
314 | - |
- |
Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone phosphate + NAD(P)H | formation of glycerol-1-phosphate is the natural direction of the reaction | Methanothermobacter thermautotrophicus | sn-glycerol-1-phosphate + NAD(P)+ | - |
r | |
dihydroxyacetonephosphate + NAD(P)H | - |
Methanothermobacter thermautotrophicus | glycerol-1-phosphate + NAD(P)+ | - |
? | |
sn-glycerol-1-phosphate + NAD+ | - |
Methanothermobacter thermautotrophicus | dihydroxyacetone phosphate + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 38000, SDS-PAGE | Methanothermobacter thermautotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Methanothermobacter thermautotrophicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
51 | - |
temperature-induced conformational change | Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | 7.4 | - |
Methanothermobacter thermautotrophicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
decrease of activity below | Methanothermobacter thermautotrophicus |
7.4 | - |
decrease of activity above | Methanothermobacter thermautotrophicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Methanothermobacter thermautotrophicus | |
NADP+ | - |
Methanothermobacter thermautotrophicus |