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Literature summary for 1.1.1.261 extracted from

  • Nishihara, M.; Koga, Y.
    Purification and properties of sn-glycerol-1-phosphate dehydrogenase from Methanobacterium thermoautotrophicum: Characterization of the biosynthetic enzyme for the enantiomeric glycerophhosphate backbone of ether polar lipids of Archaea (1997), J. Biochem., 122, 572-576.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Co2+ slight inhibition at 1 mM Methanothermobacter thermautotrophicus
Cu2+ slight inhibition at 1 mM Methanothermobacter thermautotrophicus
DTT 45% inhibition at 1 mM Methanothermobacter thermautotrophicus
Ni2+ slight inhibition at 1 mM Methanothermobacter thermautotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
NADPH dihydroxyacetone phosphate reduction Methanothermobacter thermautotrophicus
0.127
-
NAD+ glycerol-1-phosphate oxidation Methanothermobacter thermautotrophicus
0.129
-
NADH dihydroxyacetone phosphate reduction Methanothermobacter thermautotrophicus
0.27
-
NADP+ glycerol-1-phosphate oxidation Methanothermobacter thermautotrophicus
0.58
-
dihydroxyacetone phosphate in the presence of NADPH Methanothermobacter thermautotrophicus
2.17
-
dihydroxyacetone phosphate
-
Methanothermobacter thermautotrophicus
4.8
-
glycerol-1-phosphate in the presence of NADPH Methanothermobacter thermautotrophicus
16.3
-
glycerol-1-phosphate
-
Methanothermobacter thermautotrophicus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ maximum activity at 60-80 mM Methanothermobacter thermautotrophicus
Na+ maximum activity 20-25 mM Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
8 * 38000, SDS-PAGE Methanothermobacter thermautotrophicus
302000
-
gel filtration Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydroxyacetone phosphate + NAD(P)H Methanothermobacter thermautotrophicus formation of glycerol-1-phosphate is the natural direction of the reaction sn-glycerol-1-phosphate + NAD(P)+
-
r
sn-glycerol-1-phosphate + NAD+ Methanothermobacter thermautotrophicus
-
dihydroxyacetone phosphate + NADH
-
r

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 7step chromatography Methanothermobacter thermautotrophicus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Methanothermobacter thermautotrophicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
314
-
-
Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroxyacetone phosphate + NAD(P)H formation of glycerol-1-phosphate is the natural direction of the reaction Methanothermobacter thermautotrophicus sn-glycerol-1-phosphate + NAD(P)+
-
r
dihydroxyacetonephosphate + NAD(P)H
-
Methanothermobacter thermautotrophicus glycerol-1-phosphate + NAD(P)+
-
?
sn-glycerol-1-phosphate + NAD+
-
Methanothermobacter thermautotrophicus dihydroxyacetone phosphate + NADH
-
r

Subunits

Subunits Comment Organism
octamer 8 * 38000, SDS-PAGE Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Methanothermobacter thermautotrophicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
51
-
temperature-induced conformational change Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6 7.4
-
Methanothermobacter thermautotrophicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.6
-
decrease of activity below Methanothermobacter thermautotrophicus
7.4
-
decrease of activity above Methanothermobacter thermautotrophicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Methanothermobacter thermautotrophicus
NADP+
-
Methanothermobacter thermautotrophicus