Cloned (Comment) | Organism |
---|---|
gene aroE, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
A243G | site-directed mutagenesis, the mutant shows altered cofactor specificity compared to wild-type enzyme | Escherichia coli |
D195E | site-directed mutagenesis, the mutant shows altered cofactor specificity compared to wild-type enzyme | Escherichia coli |
additional information | invertion of the cofactor specificity from NADP+ to NAD+ on the Escherichia coli wild-type enzyme, effect of consensus mutations, overview. Mutant structure modeling | Escherichia coli |
N149D | site-directed mutagenesis, the mutant shows altered cofactor specificity compared to wild-type enzyme | Escherichia coli |
N149D/V152F | site-directed mutagenesis, the mutant shows altered cofactor specificity compared to wild-type enzyme | Escherichia coli |
S131A | site-directed mutagenesis, the mutant shows altered cofactor specificity compared to wild-type enzyme | Escherichia coli |
S131A/L135A | site-directed mutagenesis, the mutant shows altered cofactor specificity compared to wild-type enzyme | Escherichia coli |
S131A/L135A/N149D/V152F | site-directed mutagenesis, the mutant shows altered cofactor specificity compared to wild-type enzyme | Escherichia coli |
S131A/N149D/V152F | site-directed mutagenesis, the mutant shows altered cofactor specificity compared to wild-type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Escherichia coli | |
0.13 | - |
shikimate | pH 9.0, 25°C, recombinant wild-type enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
shikimate + NAD+ | Escherichia coli | - |
3-dehydroshikimate + NADH + H+ | - |
r | |
shikimate + NADP+ | Escherichia coli | - |
3-dehydroshikimate + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P15770 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography and dialysis | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
shikimate + NAD+ | - |
Escherichia coli | 3-dehydroshikimate + NADH + H+ | - |
r | |
shikimate + NADP+ | - |
Escherichia coli | 3-dehydroshikimate + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AroE | - |
Escherichia coli |
EcoSDH | - |
Escherichia coli |
SDH | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
3-dehydroshikimate formation, assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
190 | - |
shikimate | pH 9.0, 25°C, recombinant wild-type enzyme | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
3-dehydroshikimate formation, assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli | |
NADH | - |
Escherichia coli | |
NADP+ | preferred cofactor of the wild-type enzyme | Escherichia coli | |
NADPH | - |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.322 | - |
NADP+ | pH 9.0, 25°C, recombinant mutant N149D | Escherichia coli | |
0.457 | - |
NADP+ | pH 9.0, 25°C, recombinant mutant N149D/V152F | Escherichia coli | |
1.42 | - |
NAD+ | pH 9.0, 25°C, recombinant wild-type enzyme | Escherichia coli | |
1.8 | - |
NAD+ | pH 9.0, 25°C, recombinant mutant N149D | Escherichia coli | |
2.217 | - |
NADP+ | pH 9.0, 25°C, recombinant mutant S131A/N149D/V152F | Escherichia coli | |
3.133 | - |
NADP+ | pH 9.0, 25°C, recombinant mutant S131A/L135A/N149D/V152F | Escherichia coli | |
4.17 | - |
NAD+ | pH 9.0, 25°C, recombinant mutant N149D/V152F | Escherichia coli | |
12.55 | - |
NAD+ | pH 9.0, 25°C, recombinant mutant S131A/N149D/V152F | Escherichia coli | |
16.72 | - |
NAD+ | pH 9.0, 25°C, recombinant mutant S131A/L135A/N149D/V152F | Escherichia coli | |
1461.5 | - |
shikimate | pH 9.0, 25°C, recombinant wild-type enzyme | Escherichia coli | |
3200 | - |
NADP+ | pH 9.0, 25°C, recombinant wild-type enzyme | Escherichia coli |