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Literature summary for 1.1.1.25 extracted from

  • Ding, L.; Hofius, D.; Hajirezaei, M.R.; Fernie, A.R.; Boernke, F.; Sonnewald, U.
    Functional analysis of the essential bifunctional tobacco enzyme 3-dehydroquinate dehydratase/shikimate dehydrogenase in transgenic tobacco plants (2007), J. Exp. Bot., 58, 2053-2067.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis Nicotiana tabacum
DNA and amino acid sequence determination and analysis, expression of Hs6-tagged isozyme 1 in Escherichia coli, transfection of Nicotina plants Nicotiana tabacum
expression in Escherichia coli Nicotiana tabacum

Protein Variants

Protein Variants Comment Organism
additional information cytosolic isoform cannot complement loss of the plastidial isoform Nicotiana tabacum
additional information expression of endogenous DHD/SHD-1 is suppressed by RNAi in transgenic tobacco plants, the transgenic lines with less than 40% of wild-type activity display severe growth retardation and reduced content of aromatic amino acids and downstream products such as cholorogenic acid and lignin, but accumulation of dehydroquinate and shikimate, possibly due to existence of a parallel extra-plastidic shikimate pathway into which dehydroquinate is diverted with a second gene DHD/SHD-2 in tobacco lacking a plastidic targeting sequence, the cytosolic shikimate synthesis cannot complement loss of the plastidial pathway, phenotype, overview Nicotiana tabacum
additional information suppression of shikimate dehydrogenase activity by RNAi. Transgenic lines with less than 40% of wild-type activity display severe growth retardation and reduced content of aromatic amino acids and downstream products such as cholorogenic acid and lignin. Dehydroquinate, the substrate of the enzyme, accumulates, and due to a second, cytosolic enzyme, the product, shikimate accumulates Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
NADP+ 22°C, pH 9.0 Nicotiana tabacum
0.13
-
shikimate 22°C, pH 9.0 Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast DHD/SHD-1 containing a plastidic targeting sequence Nicotiana tabacum 9507
-
cytosol DHD/SHD-2 Nicotiana tabacum 5829
-
cytosol exclusively cytosolic Nicotiana tabacum 5829
-
plastid
-
Nicotiana tabacum 9536
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-quinate + NADP+ Nicotiana tabacum
-
3-dehydroquinate + NADPH + H+
-
r
additional information Nicotiana tabacum the cytosolic shikimate synthesis cannot complement loss of the plastidial pathway ?
-
?
shikimate + NAD+ Nicotiana tabacum
-
3-dehydroshikimate + NADH + H+
-
r
shikimate + NADP+ Nicotiana tabacum
-
3-dehydroshikimate + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-
Nicotiana tabacum Q6PUF9 DHD/SHD-1; plastidic isozyme
-
Nicotiana tabacum Q6PUG0 cytosolic isoform
-
Nicotiana tabacum Q6PUG0 DHD/SHD-2; cytosolic isozyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Nicotiana tabacum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-dehydroshikimate + NADPH
-
Nicotiana tabacum shikimate + NADP+
-
?
L-quinate + NADP+
-
Nicotiana tabacum 3-dehydroquinate + NADPH + H+
-
r
additional information the cytosolic shikimate synthesis cannot complement loss of the plastidial pathway Nicotiana tabacum ?
-
?
additional information the enzyme also shows 3-dehydroquinate dehydratase activity, EC 4.2.1.10 Nicotiana tabacum ?
-
?
shikimate + NAD+
-
Nicotiana tabacum 3-dehydroshikimate + NADH + H+
-
r
shikimate + NADP+
-
Nicotiana tabacum 3-dehydroshikimate + NADPH + H+
-
?
shikimate + NADP+
-
Nicotiana tabacum 3-dehydroshikimate + NADPH + H+
-
r

Synonyms

Synonyms Comment Organism
3-dehydroquinate dehydratase/shikimate dehydrogenase
-
Nicotiana tabacum
DHD/SHD
-
Nicotiana tabacum
More cf. EC 1.1.1.25 Nicotiana tabacum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Nicotiana tabacum
30 45
-
Nicotiana tabacum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.2 formation of shikimate Nicotiana tabacum
9 9.4 formation of 3-dehydroshikimate Nicotiana tabacum
9
-
assay at Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Nicotiana tabacum
NADPH
-
Nicotiana tabacum