Literature summary for 1.1.1.25 extracted from

Zhang, X.; Zhang, S.; Hao, F.; Lai, X.; Yu, H.; Huang, Y.; Wang, H.
Expression, purification and properties of shikimate dehydrogenase from Mycobacterium tuberculosis (2005), J. Biochem. Mol. Biol., 38, 624-631.

Search for more literature for 1.1.1.25

Activating Compound

Activating Compound	Comment	Organism	Structure
Nonidet P-40	120-190% activity of AroE at 0.1-0.5%	Mycobacterium tuberculosis
Triton X-100	120-190% activity at 0.1-0.5%	Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment)	Organism
gene aroE, expression of His-tagged AroE in Escherichia coli strain BL21(DE3)	Mycobacterium tuberculosis

General Stability

General Stability	Organism
the purified recombinant enzyme is stable to freezing	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
SDS	nearly complete inactivation of AroE at 0.02%	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetic profile of the recombinant AroE, overview	Mycobacterium tuberculosis
0.0295	-	NADP+	pH 9.0, 25°C	Mycobacterium tuberculosis
0.063	-	shikimate	pH 9.0, 25°C	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
shikimate + NADP+	Mycobacterium tuberculosis	-	3-dehydroshikimate + NADPH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	gene aroE	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged ARoE from Escherichia coli strain BL21(DE3) by nickel affinity chromatograohy	Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
826	-	purified recombinant AroE	Mycobacterium tuberculosis

Storage Stability

Storage Stability	Organism
-70°C, purified recombinant enzyme, stable for several months	Mycobacterium tuberculosis
4°C, purified recombinant enzyme, stable for 2 weeks	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
shikimate + NADP+	-	Mycobacterium tuberculosis	3-dehydroshikimate + NADPH + H+	-	r

Subunits

Subunits	Comment	Organism
More	secondary structure analysis of ARoE, percentages for alpha-helix, beta-sheet, beta-turn, and random coil are 29.2%, 9.3%, 32.7%, and 28.8%, respectively, contains the highly conserved motif G-X-(N/S)-V-(T/S)-X-PX-K	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
AroE	-	Mycobacterium tuberculosis
SD	-	Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
63	-	-	Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
87	-	purified recombinant AroE, half-life: 2 h	Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
399	-	NADP+	pH 9.0, 25°C	Mycobacterium tuberculosis
399	-	shikimate	pH 9.0, 25°C	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	12	-	Mycobacterium tuberculosis

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Mycobacterium tuberculosis
NADPH	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)