Cloned (Comment) | Organism |
---|---|
gene aroE, expression of GST-tagged SeMet-labeled enzyme in Escherichia coli strain BL21 | Methanocaldococcus jannaschii |
Crystallization (Comment) | Organism |
---|---|
in complex with NADP+, compact alpha/beta sandwich with two domains for binding substrate and cofactor, resp. | Methanocaldococcus jannaschii |
purified recombinant AroE bound to cofactor NADP+, hanging drop vapour diffusion method at 25°C, 10 mg/ml protein in 20 mM HEPES, pH 7.1, and 0.1 M KCl, is mixed with reservoir solution containing 20 mM HEPES, pH 7.1, 20% PEG 3350, and 0.2 M ammonium fluoride, cryoprotection with 15% glycerol, X-ray diffraction structure determination and analysis at 2.35 A resolution | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31690 | - |
recombinant AroE, mass spectrometry | Methanocaldococcus jannaschii |
31691 | - |
x * 31691, recombinant AroE, mass spectrometry | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
shikimate + NADP+ | Methanocaldococcus jannaschii | - |
3-dehydroshikimate + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58484 | - |
- |
Methanocaldococcus jannaschii | Q58484 | gene aroE | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged SeMet-labeled enzyme from Escherichia coli strain BL21 by glutathione affinity and ion exchange chromatography, proteolytic removal of the GST-tag | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
shikimate + NADP+ | - |
Methanocaldococcus jannaschii | 3-dehydroshikimate + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 31691, recombinant AroE, mass spectrometry | Methanocaldococcus jannaschii |
More | the enzyme shows an alpha/beta sandwich with two distinct domains, responsible for binding substrate and the NADP cofactor, respectively, a phylogenetically conserved deep cleft on the protein surface corresponds to the enzyme active site, the structure reveals a topologically unique domain fold within the N-terminal segment of the polypeptide chain, which binds substrate and supports dimerization, homology modeling, overview | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
SDH | - |
Methanocaldococcus jannaschii |
shikimate 5-dehydrogenase | - |
Methanocaldococcus jannaschii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Methanocaldococcus jannaschii | |
NADPH | - |
Methanocaldococcus jannaschii |