Cloned (Comment) | Organism |
---|---|
expression of wild-type and selenomethionine-labeled enzyme in Escherichia coli strain DL41 | Haemophilus influenzae |
Crystallization (Comment) | Organism |
---|---|
catalytic domain with open twisted alpha/beta motif plus NADPH binding domain with typical Rossman fold | Haemophilus influenzae |
wild-type and selenomethionine-labeled enzyme, free and in complex with NADPH, sitting drop vapour diffusion method, 10 mg/m protein, with or without 5 mM NADPH, mixture in a ratio of 1:1 with crystallization solution containing 1.0 M sodium citrate, and 0.1 M 2-(cyclohexylamino)ethanesulfonic acid (CHES), pH 8.8, for the free enzyme, and 12% PEG 8000, 0.15 M calcium acetate, pH 7.8, and 0.1 M imidazole for the selenomethionine-labeled enzyme, 4°C, X-ray diffraction structure determination and analysis at 2.4 A and 1.95 A, respectively, structure modeling | Haemophilus influenzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
shikimate + NADP+ | Haemophilus influenzae | - |
3-dehydroshikimate + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | - |
recombinant enzyme expressed in Escherichia coli | - |
Haemophilus influenzae | P43876 | gene aroE; gene aroE | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
shikimate + NADP+ | - |
Haemophilus influenzae | 3-dehydroshikimate + NADPH + H+ | - |
r | |
shikimate + NADP+ | - |
Haemophilus influenzae | 3-dehydroshikimate + NADPH + H+ | analysis of the 3-dehydroshikimate binding site | r |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional structure analysis of AroE | Haemophilus influenzae |
Synonyms | Comment | Organism |
---|---|---|
AroE | - |
Haemophilus influenzae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Haemophilus influenzae | |
NADPH | binding site determinatiion and binding mode analysis, AroE contains a Rossmann fold NADPH binding domain | Haemophilus influenzae |