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show all sequences of 1.1.1.245

Temperature-induced conformational change at the catalytic site of Sulfolobus solfataricus alcohol dehydrogenase highlighted by Asn249Tyr substitution. A hydrogen/deuterium exchange, kinetic, and fluorescence quenching study

Secundo, F.; Russo, C.; Giordano, A.; Carrea, G.; Rossi, M.; Raia, C.A.; Biochemistry 44, 11040-11048 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli
Saccharolobus solfataricus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37500
-
4 * 37500, SDS-PAGE
Saccharolobus solfataricus
150000
-
SDS-PAGE
Saccharolobus solfataricus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharolobus solfataricus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Saccharolobus solfataricus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-propanol + NAD+
-
667621
Saccharolobus solfataricus
propionaldehyde + NADH
-
-
-
?
benzyl alcohol + NAD+
-
667621
Saccharolobus solfataricus
benzylaldehyde + NADH
-
-
-
?
cyclohexanol + NAD+
-
667621
Saccharolobus solfataricus
cyclohexanone + NADH + H+
-
-
-
?
cyclohexanol + NAD+
temperature-induced conformational change is associated with the flexible loops directly involved in the substrate and coenzyme binding
667621
Saccharolobus solfataricus
cyclohexanone + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 37500, SDS-PAGE
Saccharolobus solfataricus
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
65
-
Saccharolobus solfataricus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
; temperature-induced conformational change is associated with the flexible loops directly involved in the substrate and coenzyme binding
Saccharolobus solfataricus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Saccharolobus solfataricus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
; temperature-induced conformational change is associated with the flexible loops directly involved in the substrate and coenzyme binding
Saccharolobus solfataricus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37500
-
4 * 37500, SDS-PAGE
Saccharolobus solfataricus
150000
-
SDS-PAGE
Saccharolobus solfataricus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Saccharolobus solfataricus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-propanol + NAD+
-
667621
Saccharolobus solfataricus
propionaldehyde + NADH
-
-
-
?
benzyl alcohol + NAD+
-
667621
Saccharolobus solfataricus
benzylaldehyde + NADH
-
-
-
?
cyclohexanol + NAD+
-
667621
Saccharolobus solfataricus
cyclohexanone + NADH + H+
-
-
-
?
cyclohexanol + NAD+
temperature-induced conformational change is associated with the flexible loops directly involved in the substrate and coenzyme binding
667621
Saccharolobus solfataricus
cyclohexanone + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 37500, SDS-PAGE
Saccharolobus solfataricus
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
65
-
Saccharolobus solfataricus
Other publictions for EC 1.1.1.245
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742146
Karande
Biocatalytic conversion of cy ...
Acidovorax sp.
Biotechnol. Bioeng.
115
312-320
2017
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
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-
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-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667502
Giordano
Evidence for co-operativity in ...
Saccharolobus solfataricus
Biochem. J.
388
657-667
2005
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667621
Secundo
Temperature-induced conformati ...
Saccharolobus solfataricus
Biochemistry
44
11040-11048
2005
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
4
1
-
1
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
4
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
286383
Tae-Kang
-
Purification and characterizat ...
Rhodococcus sp., Rhodococcus sp. TK6
J. Microbiol. Biotechnol.
12
39-45
2002
-
-
-
-
-
-
6
5
-
2
2
-
-
2
-
-
1
-
-
-
1
-
30
1
-
-
3
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
6
-
5
-
2
2
-
-
-
-
1
-
-
1
-
30
1
-
-
3
-
-
-
1
-
-
-
-
-
-
-
12556
Dangel
-
Enzyme reactions involved in a ...
Pseudomonas sp.
Arch. Microbiol.
152
273-279
1989
-
-
-
-
-
-
-
2
-
-
-
2
-
1
-
-
-
-
-
-
3
1
14
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
3
1
14
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
286378
Trower
Isolation and characterization ...
Xanthobacter sp.
Appl. Environ. Microbiol.
49
1282-1289
1985
-
-
-
-
-
-
-
4
-
-
-
1
-
1
-
-
-
-
-
-
1
-
6
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
-
-
1
-
-
-
-
-
-
1
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286381
Stirling
-
Purification and properties of ...
Nocardia sp.
Curr. Microbiol.
4
37-40
1980
-
-
-
-
-
-
1
2
-
-
1
-
-
1
-
-
1
-
-
-
1
-
25
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
1
-
-
1
-
25
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
286382
Anderson M.A.; Hall
-
Microbial metabolism of alicyc ...
Pseudomonas sp.
J. Gen. Microbiol.
120
89-94
1980
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
246371
Donoghue
The metabolism of cyclohexanol ...
Acinetobacter sp., Acinetobacter sp. NCIB 9871
Eur. J. Biochem.
60
1-7
1975
-
-
-
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
4
-
-
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-
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