Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | almost complete inhibition of activity at 10 mM | Rhodococcus sp. | |
Ca2+ | inhibition of activity at 10 mM | Rhodococcus sp. | |
Co2+ | inhibition of activity at 10 mM | Rhodococcus sp. | |
Cu2+ | almost complete inhibition of activity at 10 mM | Rhodococcus sp. | |
Mn2+ | 93% inhibition of activity at 10 mM | Rhodococcus sp. | |
Zn2+ | 91% inhibition of activity at 10 mM | Rhodococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
Cyclohexanol | isoenzyme II at pH 7.6 | Rhodococcus sp. | |
0.0028 | - |
trans-Cyclohexane-1,2-diol | isoenzyme II at pH 7.6 | Rhodococcus sp. | |
0.0135 | - |
hexane-1,2-diol | isoenzyme II at pH 7.6 | Rhodococcus sp. | |
0.0137 | - |
trans-cyclopentane-1,2-diol | isoenzyme II at pH 7.6 | Rhodococcus sp. | |
0.0142 | - |
Cyclopentanol | isoenzyme II at pH 7.6 | Rhodococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | increase of activity at 10 mM | Rhodococcus sp. | |
Mg2+ | increase of activity at 10 mM | Rhodococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000, SDS-PAGE, isoenzyme II | Rhodococcus sp. |
60000 | - |
gel filtration, isoenzyme II | Rhodococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | - |
- |
- |
Rhodococcus sp. TK6 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
9.8fold, isoenzyme II | Rhodococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
127 | - |
purified enzyme | Rhodococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butanol + NAD+ | relative activity at 20% the rate of cyclohexanol oxidation | Rhodococcus sp. | butanal + NADH | - |
? | |
1-hexanol + NAD+ | relative oxidation activity at 21% the rate of cyclohexanol oxidation | Rhodococcus sp. | hexanal + NADH | - |
? | |
1-pentanol + NAD+ | relative activity at 23% the rate of cyclohexanol oxidation | Rhodococcus sp. | n-pentanal + NADH | - |
? | |
1-propanol + NAD+ | relative activity at 20% the rate of cyclohexanol oxidation | Rhodococcus sp. | propionaldehyde + NADH | - |
? | |
2-butanol + NAD+ | relative activity at 44% the rate of cyclohexanol oxidation | Rhodococcus sp. | 2-butanone + NADH | - |
? | |
2-methyl-1-butanol + NAD+ | relative activity at 26% the rate of cyclohexanol oxidation | Rhodococcus sp. | 2-methyl-1-butanal + NADH | - |
? | |
2-methyl-1-propanol + NAD+ | relative activity at 17% the rate of cyclohexanol oxidation | Rhodococcus sp. | isobutyraldehyde + NADH | - |
? | |
2-methyl-2-propanol + NAD+ | relative activity at 16% the rate of cyclohexanol oxidation | Rhodococcus sp. | ? + NADH | - |
? | |
2-propanol + NAD+ | relative activity at 17% the rate of cyclohexanol oxidation | Rhodococcus sp. | acetone + NADH | - |
? | |
cis-cyclopentane-1,2-diol + NAD+ | relative activity at 27% the rate of cyclohexanol oxidation | Rhodococcus sp. | ? + NADH | - |
? | |
cyclohexan-1,2-diol + NAD+ | relative oxidation activity of cis-isomer at 28% the rate of cyclohexanol oxidation | Rhodococcus sp. | cyclohexane-1,2-dione + NADH | - |
? | |
cyclohexan-1,2-diol + NAD+ | relative oxidation activity of cis/trans mixture at 123% the rate of cyclohexanol oxidation | Rhodococcus sp. | cyclohexane-1,2-dione + NADH | - |
? | |
cyclohexan-1,2-diol + NAD+ | relative oxidation activity of trans-isomer at 146% the rate of cyclohexanol oxidation | Rhodococcus sp. | cyclohexane-1,2-dione + NADH | - |
? | |
cyclohexan-1,2-diol + NAD+ | relative oxidation activity of cis-isomer at 28% the rate of cyclohexanol oxidation | Rhodococcus sp. TK6 | cyclohexane-1,2-dione + NADH | - |
? | |
cyclohexane-1,3-diol + NAD+ | relative oxidation activity at 28% the rate of cyclohexanol oxidation | Rhodococcus sp. | cyclohexane-1,3-dione + NADH | - |
? | |
cyclohexane-1,4-diol + NAD+ | relative oxidation activity at 22% the rate of cyclohexanol oxidation | Rhodococcus sp. | cyclohexane-1,4-dione + NADH | - |
? | |
cyclohexanol + NAD+ | - |
Rhodococcus sp. | cyclohexanone + NADH | - |
? | |
cyclohexanol + NAD+ | - |
Rhodococcus sp. TK6 | cyclohexanone + NADH | - |
? | |
cyclopentanol + NAD+ | relative oxidation activity at 77% the rate of cyclohexanol oxidation | Rhodococcus sp. | cyclopentanone + NADH | - |
? | |
cyclopentanol + NAD+ | relative oxidation activity at 77% the rate of cyclohexanol oxidation | Rhodococcus sp. TK6 | cyclopentanone + NADH | - |
? | |
ethane-1,2-diol + NAD+ | relative activity at 5% the rate of cyclohexanol oxidation | Rhodococcus sp. | glyoxal + NADH | - |
? | |
ethanol + NAD+ | relative activity at 20% the rate of cyclohexanol oxidation | Rhodococcus sp. | acetaldehyde + NADH | - |
? | |
ethanol + NAD+ | relative activity at 20% the rate of cyclohexanol oxidation | Rhodococcus sp. TK6 | acetaldehyde + NADH | - |
? | |
hexane-1,2-diol + NAD+ | relative activity at 84% the rate of cyclohexanol oxidation | Rhodococcus sp. | 2-oxohexanal + NADH | - |
? | |
hexane-1,6-diol + NAD+ | relative activity at 21% the rate of cyclohexanol oxidation | Rhodococcus sp. | hexanedial + NADH | - |
? | |
methanol + NAD+ | relative activity at 2% the rate of cyclohexanol oxidation | Rhodococcus sp. | formaldehyde + NADH + H+ | - |
? | |
methanol + NAD+ | relative activity at 2% the rate of cyclohexanol oxidation | Rhodococcus sp. TK6 | formaldehyde + NADH + H+ | - |
? | |
propane-1,2-diol + NAD+ | relative activity at 30% the rate of cyclohexanol oxidation | Rhodococcus sp. | methylglyoxal + NADH | - |
? | |
propane-1,3-diol + NAD+ | relative activity at 7% the rate of cyclohexanol oxidation | Rhodococcus sp. | ? + NADH | - |
? | |
trans-cyclohexane-1,2-diol + NAD+ | - |
Rhodococcus sp. | cyclohexane-1,2-dione + NADH + H+ | - |
? | |
trans-cyclopentane-1,2-diol + NAD+ | relative activity at 114% the rate of cyclohexanol oxidation | Rhodococcus sp. | ? + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28000, SDS-PAGE, isoenzyme II | Rhodococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
cyclohexanol dehydrogenase II | isoenzyme | Rhodococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
labile | Rhodococcus sp. |
40 | - |
90% activity after incubation for 20 min | Rhodococcus sp. |
50 | - |
less than 20% activity after incubation for 20 min | Rhodococcus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | stable | Rhodococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | isoenzyme II | Rhodococcus sp. |