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Literature summary for 1.1.1.24 extracted from

  • Cain, R.B.
    The identity of shikimate dehydrogenase and quinate dehydrogenase in Aspergillus niger (1972), Biochem. J., 127, 15P.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information enzyme knockout results in loss of the ability to grow on quinate and/or shikimate Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
chloromercuribenzene 4-sulfonate inhibits both activities of the enzyme Aspergillus niger
additional information the enzyme is strongly repressed by D-glucose Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
NAD+
-
Aspergillus niger
0.26
-
NAD+ + quinate Aspergillus niger
0.32 0.37 quinate
-
Aspergillus niger
0.42
-
NAD+ + shikimate Aspergillus niger
6.25
-
quinate
-
Aspergillus niger
11.5
-
shikimate
-
Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-quinate + NAD+ Aspergillus niger
-
3-dehydroquinate + NADH + H+
-
r
additional information Aspergillus niger addition of either shikimate or quinate to the assay does not increase the reaction rate when the other substrate is present alone ?
-
?
shikimate + NAD+ Aspergillus niger
-
3-dehydroshikimate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger
-
bifunctional enzyme: quinate (shikimate) dehydrogenase, different from EC 1.1.1.25
-

Purification (Commentary)

Purification (Comment) Organism
separation from NADP+-linked shikimate dehydrogenase, EC 1.1.1.25, gel filtration Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-quinate + NAD+
-
Aspergillus niger 3-dehydroquinate + NADH + H+
-
r
additional information addition of either shikimate or quinate to the assay does not increase the reaction rate when the other substrate is present alone Aspergillus niger ?
-
?
additional information the bifunctional enzyme shows quinate and shikimate dehydrogenase activities Aspergillus niger ?
-
?
quinate + NAD+
-
Aspergillus niger 5-dehydroquinate + NADH + H+
-
?
shikimate + NAD+
-
Aspergillus niger ?
-
?
shikimate + NAD+
-
Aspergillus niger 3-dehydroshikimate + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
quinate dehydrogenase
-
Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
47
-
inactivation, kinetics vary in presence of quinate or shikimate, respectively Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
different pH optima for shikimate and quinate Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADP(H) Aspergillus niger
NAD+
-
Aspergillus niger
NADH
-
Aspergillus niger