Cloned (Comment) | Organism |
---|---|
gene AKR1C35, cloned from liver, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS | Mesocricetus auratus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hexestrol | - |
Mesocricetus auratus | |
hinokitiol | - |
Mesocricetus auratus | |
additional information | no or poor inhibition by 7-hydroxyflavone, chrysin, quercetin, genistein, galangin, lithocholic acid, and zearalenone | Mesocricetus auratus | |
Phenolphthalein | - |
Mesocricetus auratus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Mesocricetus auratus | |
0.0012 | - |
geranylgeraniol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0097 | - |
beta-ionol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.011 | - |
farnesol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.013 | - |
beta-3-hydroxyhexobarbital | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.013 | - |
NADH | pH 7.4, 25°C, with substrate S-camphorquinone | Mesocricetus auratus | |
0.013 | - |
NAD+ | pH 7.4, 25°C, with substrate S-tetralol | Mesocricetus auratus | |
0.03 | - |
alpha-3-hydroxyhexobarbital | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.061 | - |
(S)-1,2,3,4-tetrahydro-1-naphthol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.076 | - |
geraniol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.095 | - |
(R)-1,2,3,4-tetrahydro-1-naphthol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.148 | - |
1-Acenaphthenol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.162 | - |
nerol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.181 | - |
(S)-1-indanol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.569 | - |
2-Cyclohexen-1-ol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.63 | - |
cis-benzene dihydrodiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
1 | - |
trans-benzene dihydrodiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
1.38 | - |
NADP+ | pH 7.4, 25°C, with substrate S-camphorquinone | Mesocricetus auratus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mesocricetus auratus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
recombinant His-tagged enzyme, SDS-PAGE | Mesocricetus auratus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesocricetus auratus | A0A097ZMY7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, ultrafiltration, and dialysis | Mesocricetus auratus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | in male hamsters | Mesocricetus auratus | - |
colon | in male hamsters | Mesocricetus auratus | - |
heart | in female hamsters | Mesocricetus auratus | - |
kidney | in male hamsters | Mesocricetus auratus | - |
liver | - |
Mesocricetus auratus | - |
additional information | mRNA for AKR1C34 is expressed liver-specific in female hamsters and ubiquitously in male hamsters | Mesocricetus auratus | - |
small intestine | in male hamsters | Mesocricetus auratus | - |
testis | in male hamsters | Mesocricetus auratus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-1,2,3,4-tetrahydro-1-naphthol + NAD+ | i.e. (R)-tetralol | Mesocricetus auratus | 3,4-dihydronaphthalen-1(2H)-one + NADH + H+ | - |
? | |
(S)-1,2,3,4-tetrahydro-1-naphthol + NAD+ | i.e. (S)-tetralol | Mesocricetus auratus | 3,4-dihydronaphthalen-1(2H)-one + NADH + H+ | - |
? | |
(S)-1,2,3,4-tetrahydro-1-naphthol + NADP+ | i.e. (S)-tetralol | Mesocricetus auratus | 3,4-dihydronaphthalen-1(2H)-one + NADPH + H+ | - |
? | |
(S)-1-indanol + NAD+ | - |
Mesocricetus auratus | 2,3-dihydro-1H-inden-1-one + NADH + H+ | - |
? | |
(S)-camphorquinone + NADH + H+ | - |
Mesocricetus auratus | ? + NAD+ | - |
? | |
(S)-camphorquinone + NADPH + H+ | - |
Mesocricetus auratus | ? + NADP+ | - |
? | |
1-acenaphthenol + NAD+ | - |
Mesocricetus auratus | acenaphthylen-1(2H)-one + NADH + H+ | - |
? | |
17beta-estradiol + NAD+ | - |
Mesocricetus auratus | estrone + NADH + H+ | - |
? | |
2-cyclohexen-1-ol + NAD+ | - |
Mesocricetus auratus | 2-cyclohexen-1-one + NADH + H+ | - |
? | |
20alpha-hydroxyprogesterone + NADH + H+ | - |
Mesocricetus auratus | ? + NAD+ | - |
? | |
3alpha-hydroxyprogesterone + NADH + H+ | - |
Mesocricetus auratus | ? + NAD+ | - |
? | |
3beta-hydroxyprogesterone + NADH + H+ | - |
Mesocricetus auratus | ? + NAD+ | - |
? | |
5alpha-androstan-3alpha-ol-17-one + NAD+ | - |
Mesocricetus auratus | 5alpha-androstan-3,17-dione + NADH + H+ | - |
? | |
5alpha-androstan-3beta-ol-17-one + NAD+ | - |
Mesocricetus auratus | 5alpha-androstan-3,17-dione + NADH + H+ | - |
? | |
5alpha-androstane-3alpha,17beta-diol + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
5alpha-androstane-3beta,17beta-diol + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
5alpha-dihydrotestosterone + NAD+ | - |
Mesocricetus auratus | 5alpha-androstane-3,17-dione + NADH + H+ | - |
? | |
5alpha-pregnan-20alpha-ol-3-one + NAD+ | - |
Mesocricetus auratus | 5alpha-pregnan-3,20-dione + NADH + H+ | - |
? | |
5alpha-pregnan-3alpha-ol-20-one + NAD+ | - |
Mesocricetus auratus | 5alpha-pregnan-3,20-dione + NADH + H+ | - |
? | |
5alpha-pregnan-3beta-ol-20-one + NAD+ | - |
Mesocricetus auratus | 5alpha-pregnan-3,20-dione + NADH + H+ | - |
? | |
5beta-androstan-17beta-ol-3-one + NAD+ | - |
Mesocricetus auratus | 5beta-androstan-3,17-dione + NADH + H+ | - |
? | |
5beta-androstan-3alpha-ol-17-one + NAD+ | - |
Mesocricetus auratus | 5beta-androstan-3,17-dione + NADH + H+ | - |
? | |
5beta-androstane-3alpha,17beta-diol + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
5beta-androstane-3beta,17beta-diol + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
5beta-dihydrotestosterone + NAD+ | - |
Mesocricetus auratus | androstanedione + NADH + H+ | - |
? | |
5beta-pregnan-20alpha-ol-3-one + NAD+ | - |
Mesocricetus auratus | 5beta-pregnan-3,20-dione + NADH + H+ | - |
? | |
5beta-pregnan-3alpha-ol-20-one + NAD+ | - |
Mesocricetus auratus | 5beta-pregnan-3,20-dione + NADH + H+ | - |
? | |
5beta-pregnan-3beta,21-diol-20-one + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
5beta-pregnan-3beta-ol-20-one + NAD+ | - |
Mesocricetus auratus | 5beta-pregnan-3,20-dione + NADH + H+ | - |
? | |
alpha-3-hydroxyhexobarbital + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
beta-3-hydroxyhexobarbital + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
beta-ionol + NAD+ | - |
Mesocricetus auratus | (3E)-4-(2,6,6-trimethylcyclohex-1-en-1-yl)but-3-en-2-one + NADH + H+ | - |
? | |
cis-benzene dihydrodiol + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
dehydroepiandrosterone + NAD+ | - |
Mesocricetus auratus | ? + NADH + H+ | - |
? | |
farnesol + NAD+ | - |
Mesocricetus auratus | farnesal + NADH + H+ | - |
? | |
geraniol + NAD+ | - |
Mesocricetus auratus | geranial + NADH + H+ | - |
? | |
geranylgeraniol + NAD+ | - |
Mesocricetus auratus | geranylgeranial + NADH + H+ | - |
? | |
additional information | the enzyme AKR1C34 shows 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase activities, substrate specificity, detailed overview. Ala54 plays a critical role in non-/recognition of the steroidal substrates. Reaction products identification by thin-layer chromatography (TLC) and liquid chromatography/mass spectrometry (LC/MS). AKR1C34 also has morphine dehydrogenase activity, EC 1.1.1.218. No activity with cyclohexanol | Mesocricetus auratus | ? | - |
? | |
nerol + NAD+ | - |
Mesocricetus auratus | neral + NADH + H+ | - |
? | |
testosterone + NAD+ | - |
Mesocricetus auratus | 4-androstenedione + NADH + H+ | - |
? | |
trans-benzene dihydrodiol + NAD+ | - |
Mesocricetus auratus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, recombinant His-tagged enzyme, SDS-PAGE | Mesocricetus auratus |
Synonyms | Comment | Organism |
---|---|---|
AKR1C34 | - |
Mesocricetus auratus |
More | cf. EC 1.1.1.218, morphine 6-dehydrogenase | Mesocricetus auratus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mesocricetus auratus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
farnesol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0075 | - |
(R)-1,2,3,4-tetrahydro-1-naphthol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0153 | - |
geraniol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.025 | - |
NAD+ | pH 7.4, 25°C, with substrate S-tetralol | Mesocricetus auratus | |
0.028 | - |
2-Cyclohexen-1-ol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.032 | - |
cis-benzene dihydrodiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.038 | - |
geranylgeraniol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.04 | - |
alpha-3-hydroxyhexobarbital | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.045 | - |
trans-benzene dihydrodiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.053 | - |
beta-ionol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.0917 | - |
NADP+ | pH 7.4, 25°C, with substrate S-camphorquinone | Mesocricetus auratus | |
0.0967 | - |
beta-3-hydroxyhexobarbital | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.1 | - |
(S)-1-indanol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.105 | - |
(S)-1,2,3,4-tetrahydro-1-naphthol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.115 | - |
nerol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.167 | - |
NADH | pH 7.4, 25°C, with substrate S-camphorquinone | Mesocricetus auratus | |
0.283 | - |
1-Acenaphthenol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
substrate oxidation | Mesocricetus auratus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme shows high preference for NAD+ compared with NADP+ | Mesocricetus auratus | |
NAD+ | conserved residues Gln270 and Glu276 are critical residues for the NAD+ specificity | Mesocricetus auratus | |
NADH | - |
Mesocricetus auratus | |
NADP+ | - |
Mesocricetus auratus | |
NADPH | - |
Mesocricetus auratus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00065 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | Phenolphthalein | |
0.0032 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | hinokitiol | |
0.013 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | Hexestrol |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme AKR1C34 is a member of the aldoketo reductase (AKR) 1C subfamily in the AKR superfamily | Mesocricetus auratus |
physiological function | enzyme AKR1C34 oxidizes various xenobiotic alicyclic alcohols using NAD+ | Mesocricetus auratus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
trans-benzene dihydrodiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.05 | - |
2-Cyclohexen-1-ol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.05 | - |
cis-benzene dihydrodiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.067 | - |
nerol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.067 | - |
NADP+ | pH 7.4, 25°C, with substrate S-camphorquinone | Mesocricetus auratus | |
0.083 | - |
(R)-1,2,3,4-tetrahydro-1-naphthol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.2 | - |
geraniol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.42 | - |
farnesol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
0.55 | - |
(S)-1-indanol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
1.67 | - |
(S)-1,2,3,4-tetrahydro-1-naphthol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
1.83 | - |
1-Acenaphthenol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
2 | - |
NAD+ | pH 7.4, 25°C, with substrate S-tetralol | Mesocricetus auratus | |
2.17 | - |
alpha-3-hydroxyhexobarbital | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
5.3 | - |
beta-ionol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
7.5 | - |
beta-3-hydroxyhexobarbital | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
12.67 | - |
NADH | pH 7.4, 25°C, with substrate S-camphorquinone | Mesocricetus auratus | |
33.3 | - |
geranylgeraniol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus |