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Literature summary for 1.1.1.239 extracted from

  • Endahl, G.L.; Kochakian, C.D.; Hamm, D.
    Separation of a triphosphopyridine nucleotide-specific from a diphosphopyridine nucleotide-specific 17beta-hydroxy-(testosterone)dehydrogenase of guinea pig liver (1960), J. Biol. Chem., 235, 2793-2796.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0.1 mM, complete inhibition Cavia porcellus
CuCl2 1 mM, complete inhibition Cavia porcellus
FeCl3 10 mM, 46% inhibition Cavia porcellus
Hg2+ 0.1 mM, complete inhibition Cavia porcellus
NaCN 1 mM, 50% inhibition Cavia porcellus
p-chloromercuribenzoate 0.1 mM, complete inhibition Cavia porcellus
Sodium amytal 10 mM, 25% inhibition Cavia porcellus
ZnCl2 10 mM, 10% inhibition Cavia porcellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
testosterone
-
Cavia porcellus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
testosterone + NAD+ Cavia porcellus
-
androst-4-ene-3,17-dione + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Cavia porcellus
-

Storage Stability

Storage Stability Organism
4°C-6°C, 60 mM sodium diphosphate, pH 9.5, a few days, no loss in activity Cavia porcellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
testosterone + NAD+
-
Cavia porcellus androst-4-ene-3,17-dione + NADH + H+
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.6
-
sharp drop above Cavia porcellus

pH Range

pH Minimum pH Maximum Comment Organism
8 9.6
-
Cavia porcellus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Cavia porcellus