Literature summary for 1.1.1.239 extracted from

Stupans, I.; Kong, S.; Kirlich, S.; McKinnon, R.A.; Murray, M.
Testosterone dehydrogenase activity in koala liver: characterization of cofactor and steroid substrate differences (2000), Comp. Biochem. Physiol. B, 125, 245-250.

Search for more literature for 1.1.1.239

Inhibitors

Inhibitors	Comment	Organism	Structure
17beta-estradiol	0.175 mM, 25-40% inhibition of testosterone oxidation	Phascolarctos cinereus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0174	-	testosterone	enzyme avtivity in hepatic microsomes, one individual animal	Phascolarctos cinereus
0.0181	-	NAD+	-	Rattus norvegicus
0.0181	-	NAD+	enzyme activity in hepatic microsomes	Phascolarctos cinereus
0.0185	-	NAD+	enzyme avtivity in hepatic microsomes, one individual animal	Phascolarctos cinereus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Homo sapiens	-	-
microsome	-	Rattus norvegicus	-	-
microsome	-	Phascolarctos cinereus	-	-
microsome	-	Notamacropus eugenii	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
testosterone + NAD+	Homo sapiens	-	androst-4-ene-3,17-dione + NADH + H+	-	?
testosterone + NAD+	Rattus norvegicus	-	androst-4-ene-3,17-dione + NADH + H+	-	?
testosterone + NAD+	Phascolarctos cinereus	-	androst-4-ene-3,17-dione + NADH + H+	-	?
testosterone + NAD+	Notamacropus eugenii	-	androst-4-ene-3,17-dione + NADH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Notamacropus eugenii	-	wallaby	-
Phascolarctos cinereus	-	koala	-
Rattus norvegicus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Homo sapiens	-
liver	-	Rattus norvegicus	-
liver	-	Phascolarctos cinereus	-
liver	-	Notamacropus eugenii	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.000176	-	enzyme activity in hepatic cytosol	Notamacropus eugenii
0.00035	-	enzyme activity in hepatic cytosol	Phascolarctos cinereus
0.00044	-	enzyme activity in hepatic cytosol	Homo sapiens
0.0007	-	enzyme activity in hepatic microsomes	Notamacropus eugenii
0.00168	-	enzyme activity in hepatic microsomes	Homo sapiens
0.00283	-	enzyme activity in hepatic microsomes	Phascolarctos cinereus
0.01794	-	enzyme activity in hepatic microsomes	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
testosterone + NAD+	-	Homo sapiens	androst-4-ene-3,17-dione + NADH + H+	-	?
testosterone + NAD+	-	Rattus norvegicus	androst-4-ene-3,17-dione + NADH + H+	-	?
testosterone + NAD+	-	Phascolarctos cinereus	androst-4-ene-3,17-dione + NADH + H+	-	?
testosterone + NAD+	-	Notamacropus eugenii	androst-4-ene-3,17-dione + NADH + H+	-	?

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Homo sapiens
NAD+	-	Rattus norvegicus
NAD+	-	Phascolarctos cinereus
NAD+	-	Notamacropus eugenii

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Release 2023.1 (January 2023)