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Literature summary for 1.1.1.236 extracted from
- Protein structure modeling indicates hexahistidine-tag interference with enzyme activity (2008), Proteins, 72, 173-183.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene trII, DNA and amino acid sequence determination and analysis, expression as C- or N-terminally His-tagged enzyme in Escherichia coli strain SG13009, the His-tage posotion influences the pH dependence of the enzyme, oveview | Solanum dulcamara |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the recombinant His-tagged enzyme shows an increased substrate affinity compared to the wild-type enzyme | Solanum dulcamara |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
x * 29000, recombinant His-tagged enzyme, SDS-PAGE | Solanum dulcamara |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tropinone + NADPH + H+ | Solanum dulcamara | the enzyme is involved in tropane alkaloid biosynthesis, overview | pseudotropine + NADP+ | leading to formation of calystegines | ? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum dulcamara | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Solanum dulcamara |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with quinuclidione and 8-thiabicyclo[3.2.1]octan-2-one | Solanum dulcamara | ? | - |
? | |
| tropinone + NADPH + H+ | the enzyme is involved in tropane alkaloid biosynthesis, overview | Solanum dulcamara | pseudotropine + NADP+ | leading to formation of calystegines | ? | |
| tropinone + NADPH + H+ | active site structure and catalytic triad, the recombinant His-tagged enzyme shows 4fold higher activity compared to the wild-type enzyme, overview | Solanum dulcamara | pseudotropine + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29000, recombinant His-tagged enzyme, SDS-PAGE | Solanum dulcamara |
| More | structure modelling, overview | Solanum dulcamara |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the family of short chain dehydrogenases | Solanum dulcamara |
| pseudotropinone forming tropinone reductase | - |
Solanum dulcamara |
| TRII | - |
Solanum dulcamara |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Solanum dulcamara |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
recombinant His-tagged enzyme | Solanum dulcamara |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
the recombinant His-tagged enzyme shows a broader pH spectrum compared to the wild-type enzyme | Solanum dulcamara |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | binding site sequence, overview | Solanum dulcamara | |
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Release 2023.1 (January 2023)
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