BRENDA - Enzyme Database
show all sequences of 1.1.1.234

The sorghum gene for leaf color changes upon wounding (P) encodes a flavanone 4-reductase in the 3-deoxyanthocyanidin biosynthesis pathway

Kawahigashi, H.; Kasuga, S.; Sawada, Y.; Yonemaru, J.; Ando, T.; Kanamori, H.; Wu, J.; Mizuno, H.; Momma, M.; Fujimoto, Z.; Hirai, M.Y.; Matsumoto, T.; G3 (Bethesda) 6, 1439-1447 (2016)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene P or Sb06g029550, quantitative RT-PCR enzyme expression analysis, genetic mapping, phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Star(DE3)pLysS
Sorghum bicolor
Engineering
Protein Variants
Commentary
Organism
C252Y
cultivar Greenleaf Sb06g029550 has a point mutation that causes the amino acid change
Sorghum bicolor
additional information
analysis of Sb06g029550 in four sorghum accessions with the tan phenotype, overview
Sorghum bicolor
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37800
-
-
Sorghum bicolor
40000
-
-
Sorghum bicolor
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(2S)-flavan-4-ol + NADP+
Sorghum bicolor
-
(2S)-flavanone + NADPH + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Sorghum bicolor
C5YGL7
purple Nakei-MS3B (PP) and tan Greenleaf (pp) cultivars
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 Star(DE3)pLysS by nickel affinity chromatography
Sorghum bicolor
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Sorghum bicolor
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(2S)-flavan-4-ol + NADP+
-
740500
Sorghum bicolor
(2S)-flavanone + NADPH + H+
-
-
-
?
eriodictyol + NADP+
recombinant Sb06g029550 protein
740500
Sorghum bicolor
luteoforol + NADPH + H+
-
-
-
?
additional information
the reaction mixtures are acid-treated to convert the direct reaction products, flavan-3,4-diol, and flavan-4-ol, to their respective anthocyanidin or 3-deoxyanthocyanidins, and analyzed by LC-MS/MS
740500
Sorghum bicolor
?
-
-
-
-
naringenin + NADPH + H+
recombinant Sb06g029550 protein
740500
Sorghum bicolor
apiforol + NADP+
apiforol is converted to apigeninidin with Nar + protein + HCl treatment
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 40000, recombinant His-tagged enzyme, SDS-PAGE, x * 37800, about, sequence calculation
Sorghum bicolor
Synonyms
Synonyms
Commentary
Organism
3-deoxyanthocyanidin synthase
-
Sorghum bicolor
FNR
-
Sorghum bicolor
Sb06g029550
-
Sorghum bicolor
Sb06g029550 protein
-
Sorghum bicolor
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Sorghum bicolor
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Sorghum bicolor
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Sorghum bicolor
Cloned(Commentary) (protein specific)
Commentary
Organism
gene P or Sb06g029550, quantitative RT-PCR enzyme expression analysis, genetic mapping, phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Star(DE3)pLysS
Sorghum bicolor
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Sorghum bicolor
Engineering (protein specific)
Protein Variants
Commentary
Organism
C252Y
cultivar Greenleaf Sb06g029550 has a point mutation that causes the amino acid change
Sorghum bicolor
additional information
analysis of Sb06g029550 in four sorghum accessions with the tan phenotype, overview
Sorghum bicolor
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37800
-
-
Sorghum bicolor
40000
-
-
Sorghum bicolor
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(2S)-flavan-4-ol + NADP+
Sorghum bicolor
-
(2S)-flavanone + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 Star(DE3)pLysS by nickel affinity chromatography
Sorghum bicolor
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Sorghum bicolor
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(2S)-flavan-4-ol + NADP+
-
740500
Sorghum bicolor
(2S)-flavanone + NADPH + H+
-
-
-
?
eriodictyol + NADP+
recombinant Sb06g029550 protein
740500
Sorghum bicolor
luteoforol + NADPH + H+
-
-
-
?
additional information
the reaction mixtures are acid-treated to convert the direct reaction products, flavan-3,4-diol, and flavan-4-ol, to their respective anthocyanidin or 3-deoxyanthocyanidins, and analyzed by LC-MS/MS
740500
Sorghum bicolor
?
-
-
-
-
naringenin + NADPH + H+
recombinant Sb06g029550 protein
740500
Sorghum bicolor
apiforol + NADP+
apiforol is converted to apigeninidin with Nar + protein + HCl treatment
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 40000, recombinant His-tagged enzyme, SDS-PAGE, x * 37800, about, sequence calculation
Sorghum bicolor
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Sorghum bicolor
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Sorghum bicolor
Expression
Organism
Commentary
Expression
Sorghum bicolor
the enzyme is highly induced by wounding and pathogen invasion, analysis of 3-deoxyanthocyanidin accumulation
up
General Information
General Information
Commentary
Organism
metabolism
the enzyme, encoded by gene Sb06g029550, is involved in the 3-deoxyanthocyanidin synthesis pathway
Sorghum bicolor
additional information
homology modeling on the basis of the crystal structure of grape dihydroflavonol 4-reductase, PDB ID 3C1T
Sorghum bicolor
physiological function
upon wounding or pathogen invasion, leaves of Sorghum bicolor plants with the P gene turn purple, whereas leaves with the recessive allele turn brown or tan. This purple phenotype is determined by the production of two 3-deoxyanthocyanidins, apigeninidin and luteolinidin, which are not produced by the tan-phenotype plants
Sorghum bicolor
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme, encoded by gene Sb06g029550, is involved in the 3-deoxyanthocyanidin synthesis pathway
Sorghum bicolor
additional information
homology modeling on the basis of the crystal structure of grape dihydroflavonol 4-reductase, PDB ID 3C1T
Sorghum bicolor
physiological function
upon wounding or pathogen invasion, leaves of Sorghum bicolor plants with the P gene turn purple, whereas leaves with the recessive allele turn brown or tan. This purple phenotype is determined by the production of two 3-deoxyanthocyanidins, apigeninidin and luteolinidin, which are not produced by the tan-phenotype plants
Sorghum bicolor
Expression (protein specific)
Organism
Commentary
Expression
Sorghum bicolor
the enzyme is highly induced by wounding and pathogen invasion, analysis of 3-deoxyanthocyanidin accumulation
up
Other publictions for EC 1.1.1.234
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740494
Mizuno
Expression of flavone synthase ...
Sorghum bicolor
Front. Plant Sci.
7
1718
2016
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-
1
-
1
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-
-
-
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3
-
2
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-
1
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4
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3
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1
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1
1
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1
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3
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1
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4
-
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-
-
-
-
-
-
-
1
2
2
1
-
-
740500
Kawahigashi
The sorghum gene for leaf colo ...
Sorghum bicolor
G3 (Bethesda)
6
1439-1447
2016
-
-
1
-
2
-
-
-
-
-
2
1
-
3
-
-
1
-
-
2
-
-
4
1
4
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
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2
1
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1
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2
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-
4
1
1
-
-
-
1
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-
-
1
3
3
1
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-
725929
Li
Molecular characterization and ...
Saussurea medusa
Mol. Biol. Rep.
39
2991-2999
2012
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1
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1
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5
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5
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2
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1
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1
1
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1
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5
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2
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3
3
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654357
Fischer
Molecular cloning, substrate s ...
Malus domestica, Pyrus communis
Arch. Biochem. Biophys.
412
223-230
2003
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1
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6
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6
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2
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2
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10
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4
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2
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2
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1
2
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6
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2
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10
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4
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2
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657102
Halbwirth
-
Biochemical formation of antho ...
Zea mays
Plant Sci.
164
489-495
2003
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1
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2
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3
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1
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1
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1
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7
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1
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1
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2
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1
2
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2
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3
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1
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7
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1
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1
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286346
Stich
-
Biosynthesis of 3-deoxyanthocy ...
Sinningia cardinalis
Biochemistry
27
785-789
1988
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1
5
1
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1
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1
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1
1
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5
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1
1
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3
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3
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1
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5
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1
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1
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1
1
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5
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1
1
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-
-
-
-
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-
438999
Stich
-
Studies on columnidin biosynth ...
Columnea hybrida, Columnea hybrida Heklua
Z. Naturforsch. C
43
311-314
1988
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1
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2
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2
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1
1
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6
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1
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1
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1
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1
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1
1
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6
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1
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