Data extracted from this reference:
Cloned(Commentary)
gene P or Sb06g029550, quantitative RT-PCR enzyme expression analysis, genetic mapping, phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Star(DE3)pLysS
Sorghum bicolor
Engineering
C252Y
cultivar Greenleaf Sb06g029550 has a point mutation that causes the amino acid change
Sorghum bicolor
additional information
analysis of Sb06g029550 in four sorghum accessions with the tan phenotype, overview
Sorghum bicolor
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
(2S)-flavan-4-ol + NADP+
Sorghum bicolor
(2S)-flavanone + NADPH + H+
?
Organism
Sorghum bicolor
C5YGL7
purple Nakei-MS3B (PP) and tan Greenleaf (pp) cultivars
Purification (Commentary)
recombinant His-tagged enzyme from Escherichia coli strain BL21 Star(DE3)pLysS by nickel affinity chromatography
Sorghum bicolor
Source Tissue
Substrates and Products (Substrate)
(2S)-flavan-4-ol + NADP+
740500
Sorghum bicolor
(2S)-flavanone + NADPH + H+
?
eriodictyol + NADP+
recombinant Sb06g029550 protein
740500
Sorghum bicolor
luteoforol + NADPH + H+
?
additional information
the reaction mixtures are acid-treated to convert the direct reaction products, flavan-3,4-diol, and flavan-4-ol, to their respective anthocyanidin or 3-deoxyanthocyanidins, and analyzed by LC-MS/MS
740500
Sorghum bicolor
?
?
naringenin + NADPH + H+
recombinant Sb06g029550 protein
740500
Sorghum bicolor
apiforol + NADP+
apiforol is converted to apigeninidin with Nar + protein + HCl treatment
?
Subunits
?
x * 40000, recombinant His-tagged enzyme, SDS-PAGE, x * 37800, about, sequence calculation
Sorghum bicolor
Synonyms
3-deoxyanthocyanidin synthase
Sorghum bicolor
Sb06g029550
Sorghum bicolor
Sb06g029550 protein
Sorghum bicolor
Temperature Optimum [°C]
30
assay at
Sorghum bicolor
pH Optimum
7
assay at
Sorghum bicolor
Cofactor
Cloned(Commentary) (protein specific)
gene P or Sb06g029550, quantitative RT-PCR enzyme expression analysis, genetic mapping, phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Star(DE3)pLysS
Sorghum bicolor
Cofactor (protein specific)
Engineering (protein specific)
C252Y
cultivar Greenleaf Sb06g029550 has a point mutation that causes the amino acid change
Sorghum bicolor
additional information
analysis of Sb06g029550 in four sorghum accessions with the tan phenotype, overview
Sorghum bicolor
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
(2S)-flavan-4-ol + NADP+
Sorghum bicolor
(2S)-flavanone + NADPH + H+
?
Purification (Commentary) (protein specific)
recombinant His-tagged enzyme from Escherichia coli strain BL21 Star(DE3)pLysS by nickel affinity chromatography
Sorghum bicolor
Source Tissue (protein specific)
Substrates and Products (Substrate) (protein specific)
(2S)-flavan-4-ol + NADP+
740500
Sorghum bicolor
(2S)-flavanone + NADPH + H+
?
eriodictyol + NADP+
recombinant Sb06g029550 protein
740500
Sorghum bicolor
luteoforol + NADPH + H+
?
additional information
the reaction mixtures are acid-treated to convert the direct reaction products, flavan-3,4-diol, and flavan-4-ol, to their respective anthocyanidin or 3-deoxyanthocyanidins, and analyzed by LC-MS/MS
740500
Sorghum bicolor
?
?
naringenin + NADPH + H+
recombinant Sb06g029550 protein
740500
Sorghum bicolor
apiforol + NADP+
apiforol is converted to apigeninidin with Nar + protein + HCl treatment
?
Subunits (protein specific)
?
x * 40000, recombinant His-tagged enzyme, SDS-PAGE, x * 37800, about, sequence calculation
Sorghum bicolor
Temperature Optimum [°C] (protein specific)
30
assay at
Sorghum bicolor
pH Optimum (protein specific)
7
assay at
Sorghum bicolor
Expression
Sorghum bicolor
the enzyme is highly induced by wounding and pathogen invasion, analysis of 3-deoxyanthocyanidin accumulation
up
General Information
metabolism
the enzyme, encoded by gene Sb06g029550, is involved in the 3-deoxyanthocyanidin synthesis pathway
Sorghum bicolor
additional information
homology modeling on the basis of the crystal structure of grape dihydroflavonol 4-reductase, PDB ID 3C1T
Sorghum bicolor
physiological function
upon wounding or pathogen invasion, leaves of Sorghum bicolor plants with the P gene turn purple, whereas leaves with the recessive allele turn brown or tan. This purple phenotype is determined by the production of two 3-deoxyanthocyanidins, apigeninidin and luteolinidin, which are not produced by the tan-phenotype plants
Sorghum bicolor
General Information (protein specific)
metabolism
the enzyme, encoded by gene Sb06g029550, is involved in the 3-deoxyanthocyanidin synthesis pathway
Sorghum bicolor
additional information
homology modeling on the basis of the crystal structure of grape dihydroflavonol 4-reductase, PDB ID 3C1T
Sorghum bicolor
physiological function
upon wounding or pathogen invasion, leaves of Sorghum bicolor plants with the P gene turn purple, whereas leaves with the recessive allele turn brown or tan. This purple phenotype is determined by the production of two 3-deoxyanthocyanidins, apigeninidin and luteolinidin, which are not produced by the tan-phenotype plants
Sorghum bicolor
Expression (protein specific)
Sorghum bicolor
the enzyme is highly induced by wounding and pathogen invasion, analysis of 3-deoxyanthocyanidin accumulation
up
Other publictions for EC 1.1.1.234
740494
Mizuno
Expression of flavone synthase ...
Sorghum bicolor
Front. Plant Sci.
7
1718
2016
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740500
Kawahigashi
The sorghum gene for leaf colo ...
Sorghum bicolor
G3 (Bethesda)
6
1439-1447
2016
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1
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2
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2
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1
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3
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725929
Li
Molecular characterization and ...
Saussurea medusa
Mol. Biol. Rep.
39
2991-2999
2012
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3
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654357
Fischer
Molecular cloning, substrate s ...
Pyrus communis, Malus domestica
Arch. Biochem. Biophys.
412
223-230
2003
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1
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657102
Halbwirth
-
Biochemical formation of antho ...
Zea mays
Plant Sci.
164
489-495
2003
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286346
Stich
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Biosynthesis of 3-deoxyanthocy ...
Sinningia cardinalis
Biochemistry
27
785-789
1988
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438999
Stich
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Studies on columnidin biosynth ...
Columnea hybrida, Columnea hybrida Heklua
Z. Naturforsch. C
43
311-314
1988
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