BRENDA - Reference to 1.1.1.234; Id = 740500

gene P or Sb06g029550, quantitative RT-PCR enzyme expression analysis, genetic mapping, phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Star(DE3)pLysS

Sorghum bicolor

C252Y

cultivar Greenleaf Sb06g029550 has a point mutation that causes the amino acid change

Sorghum bicolor

additional information

analysis of Sb06g029550 in four sorghum accessions with the tan phenotype, overview

Sorghum bicolor

37800

-

Sorghum bicolor

40000

-

Sorghum bicolor

(2S)-flavan-4-ol + NADP+

Sorghum bicolor

-

(2S)-flavanone + NADPH + H+

-

?

Sorghum bicolor

C5YGL7

purple Nakei-MS3B (PP) and tan Greenleaf (pp) cultivars

-

recombinant His-tagged enzyme from Escherichia coli strain BL21 Star(DE3)pLysS by nickel affinity chromatography

Sorghum bicolor

leaf

-

Sorghum bicolor

-

(2S)-flavan-4-ol + NADP+

-

740500

Sorghum bicolor

(2S)-flavanone + NADPH + H+

-

?

eriodictyol + NADP+

recombinant Sb06g029550 protein

740500

Sorghum bicolor

luteoforol + NADPH + H+

-

?

additional information

the reaction mixtures are acid-treated to convert the direct reaction products, flavan-3,4-diol, and flavan-4-ol, to their respective anthocyanidin or 3-deoxyanthocyanidins, and analyzed by LC-MS/MS

740500

Sorghum bicolor

?

-

naringenin + NADPH + H+

recombinant Sb06g029550 protein

740500

Sorghum bicolor

apiforol + NADP+

apiforol is converted to apigeninidin with Nar + protein + HCl treatment

-

?

x * 40000, recombinant His-tagged enzyme, SDS-PAGE, x * 37800, about, sequence calculation

Sorghum bicolor

3-deoxyanthocyanidin synthase

-

Sorghum bicolor

FNR

-

Sorghum bicolor

Sb06g029550

-

Sorghum bicolor

Sb06g029550 protein

-

Sorghum bicolor

30

-

assay at

Sorghum bicolor

7

-

assay at

Sorghum bicolor

NADP+

-

Sorghum bicolor

gene P or Sb06g029550, quantitative RT-PCR enzyme expression analysis, genetic mapping, phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Star(DE3)pLysS

Sorghum bicolor

NADP+

-

Sorghum bicolor

C252Y

cultivar Greenleaf Sb06g029550 has a point mutation that causes the amino acid change

Sorghum bicolor

additional information

analysis of Sb06g029550 in four sorghum accessions with the tan phenotype, overview

Sorghum bicolor

37800

-

Sorghum bicolor

40000

-

Sorghum bicolor

(2S)-flavan-4-ol + NADP+

Sorghum bicolor

-

(2S)-flavanone + NADPH + H+

-

?

recombinant His-tagged enzyme from Escherichia coli strain BL21 Star(DE3)pLysS by nickel affinity chromatography

Sorghum bicolor

leaf

-

Sorghum bicolor

-

(2S)-flavan-4-ol + NADP+

-

740500

Sorghum bicolor

(2S)-flavanone + NADPH + H+

-

?

eriodictyol + NADP+

recombinant Sb06g029550 protein

740500

Sorghum bicolor

luteoforol + NADPH + H+

-

?

additional information

the reaction mixtures are acid-treated to convert the direct reaction products, flavan-3,4-diol, and flavan-4-ol, to their respective anthocyanidin or 3-deoxyanthocyanidins, and analyzed by LC-MS/MS

740500

Sorghum bicolor

?

-

naringenin + NADPH + H+

recombinant Sb06g029550 protein

740500

Sorghum bicolor

apiforol + NADP+

apiforol is converted to apigeninidin with Nar + protein + HCl treatment

-

?

x * 40000, recombinant His-tagged enzyme, SDS-PAGE, x * 37800, about, sequence calculation

Sorghum bicolor

30

-

assay at

Sorghum bicolor

7

-

assay at

Sorghum bicolor

the enzyme is highly induced by wounding and pathogen invasion, analysis of 3-deoxyanthocyanidin accumulation

up

metabolism

the enzyme, encoded by gene Sb06g029550, is involved in the 3-deoxyanthocyanidin synthesis pathway

Sorghum bicolor

additional information

homology modeling on the basis of the crystal structure of grape dihydroflavonol 4-reductase, PDB ID 3C1T

Sorghum bicolor

physiological function

upon wounding or pathogen invasion, leaves of Sorghum bicolor plants with the P gene turn purple, whereas leaves with the recessive allele turn brown or tan. This purple phenotype is determined by the production of two 3-deoxyanthocyanidins, apigeninidin and luteolinidin, which are not produced by the tan-phenotype plants

Sorghum bicolor

metabolism

the enzyme, encoded by gene Sb06g029550, is involved in the 3-deoxyanthocyanidin synthesis pathway

Sorghum bicolor

additional information

homology modeling on the basis of the crystal structure of grape dihydroflavonol 4-reductase, PDB ID 3C1T

Sorghum bicolor

physiological function

upon wounding or pathogen invasion, leaves of Sorghum bicolor plants with the P gene turn purple, whereas leaves with the recessive allele turn brown or tan. This purple phenotype is determined by the production of two 3-deoxyanthocyanidins, apigeninidin and luteolinidin, which are not produced by the tan-phenotype plants

Sorghum bicolor

the enzyme is highly induced by wounding and pathogen invasion, analysis of 3-deoxyanthocyanidin accumulation

up

740494

Mizuno

Expression of flavone synthase ...

Sorghum bicolor

Front. Plant Sci.

7

1718

2016

-

1

-

1

-

3

-

2

-

1

-

4

-

3

-

1

-

1

-

1

-

3

-

1

-

4

-

1

2

1

-

740500

Kawahigashi

The sorghum gene for leaf colo ...

Sorghum bicolor

G3 (Bethesda)

6

1439-1447

2016

-

1

-

2

-

2

1

-

3

-

1

-

2

-

4

1

4

1

-

1

-

1

-

1

-

2

-

2

1

-

1

-

2

-

4

1

-

1

-

1

3

1

-

725929

Li

Molecular characterization and ...

Saussurea medusa

Mol. Biol. Rep.

39

2991-2999

2012

-

1

-

1

-

5

-

5

-

2

-

1

-

1

-

1

-

5

-

2

-

3

-

654357

Fischer

Molecular cloning, substrate s ...

Malus domestica, Pyrus communis

Arch. Biochem. Biophys.

412

223-230

2003

-

1

-

6

-

6

-

2

-

2

-

10

-

4

-

2

-

2

-

1

2

-

6

-

2

-

10

-

4

-

2

-

657102

Halbwirth

-

Biochemical formation of antho ...

Zea mays

Plant Sci.

164

489-495

2003

-

1

-

2

-

3

-

1

-

1

-

1

-

7

-

1

-

1

-

2

-

1

2

-

2

-

3

-

1

-

7

-

1

-

1

-

286346

Stich

-

Biosynthesis of 3-deoxyanthocy ...

Sinningia cardinalis

Biochemistry

27

785-789

1988

-

1

5

1

-

1

-

1

-

1

-

5

-

1

-

3

-

3

-

1

-

5

-

1

-

1

-

1

-

5

-

1

-

438999

Stich

-

Studies on columnidin biosynth ...

Columnea hybrida, Columnea hybrida Heklua

Z. Naturforsch. C

43

311-314

1988

-

1

-

2

-

2

-

1

-

6

-

1

-

1

-

1

-

1

-

2

-

1

-

6

-

1

-

The sorghum gene for leaf color changes upon wounding (P) encodes a flavanone 4-reductase in the 3-deoxyanthocyanidin biosynthesis pathway

Data extracted from this reference: