BRENDA - Enzyme Database
show all sequences of 1.1.1.234

Expression of flavone synthase II and flavonoid 3'-hydroxylase is associated with color variation in tan-colored injured leaves of Sorghum

Mizuno, H.; Yazawa, T.; Kasuga, S.; Sawada, Y.; Kanamori, H.; Ogo, Y.; Hirai, M.Y.; Matsumoto, T.; Kawahigashi, H.; Front. Plant Sci. 7, 1718 (2016)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene P or Sb06g029550, genetic structure, quantitative RT-PCR enzyme expression analysis
Sorghum bicolor
Engineering
Protein Variants
Commentary
Organism
additional information
expression of both flavone synthase II (FNSII) and flavonoid 3'-hydroxylase (F3'H) leads to the synthesis of apigenin and luteolin in cultivar #7, and expression of both flavanone 4-reductase and F3'H leads to the synthesis of 3-deoxyanthocyanidins in cultivar Nakei-MS3B
Sorghum bicolor
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(2S)-flavan-4-ol + NADP+
Sorghum bicolor
-
(2S)-flavanone + NADPH + H+
-
-
?
eriodictyol + NADP+
Sorghum bicolor
-
luteoforol + NADPH + H+
-
-
?
naringenin + NADPH + H+
Sorghum bicolor
-
apiforol + NADP+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Sorghum bicolor
C5YGL7
cultivars #7, M36001, and Nakei
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Sorghum bicolor
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(2S)-flavan-4-ol + NADP+
-
740494
Sorghum bicolor
(2S)-flavanone + NADPH + H+
-
-
-
?
eriodictyol + NADP+
-
740494
Sorghum bicolor
luteoforol + NADPH + H+
-
-
-
?
additional information
enzyme FNR converts flavanones to flavan-4-ols
740494
Sorghum bicolor
?
-
-
-
-
naringenin + NADPH + H+
-
740494
Sorghum bicolor
apiforol + NADP+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
3-deoxyanthocyanidin synthase
-
Sorghum bicolor
FNR
-
Sorghum bicolor
Sb06g029550
-
Sorghum bicolor
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Sorghum bicolor
Cloned(Commentary) (protein specific)
Commentary
Organism
gene P or Sb06g029550, genetic structure, quantitative RT-PCR enzyme expression analysis
Sorghum bicolor
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Sorghum bicolor
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
expression of both flavone synthase II (FNSII) and flavonoid 3'-hydroxylase (F3'H) leads to the synthesis of apigenin and luteolin in cultivar #7, and expression of both flavanone 4-reductase and F3'H leads to the synthesis of 3-deoxyanthocyanidins in cultivar Nakei-MS3B
Sorghum bicolor
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(2S)-flavan-4-ol + NADP+
Sorghum bicolor
-
(2S)-flavanone + NADPH + H+
-
-
?
eriodictyol + NADP+
Sorghum bicolor
-
luteoforol + NADPH + H+
-
-
?
naringenin + NADPH + H+
Sorghum bicolor
-
apiforol + NADP+
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Sorghum bicolor
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(2S)-flavan-4-ol + NADP+
-
740494
Sorghum bicolor
(2S)-flavanone + NADPH + H+
-
-
-
?
eriodictyol + NADP+
-
740494
Sorghum bicolor
luteoforol + NADPH + H+
-
-
-
?
additional information
enzyme FNR converts flavanones to flavan-4-ols
740494
Sorghum bicolor
?
-
-
-
-
naringenin + NADPH + H+
-
740494
Sorghum bicolor
apiforol + NADP+
-
-
-
?
Expression
Organism
Commentary
Expression
Sorghum bicolor
the enzyme is highly induced by cutting stress
up
General Information
General Information
Commentary
Organism
malfunction
Sorghums with a tan injury response have non-functional FNR alleles (p). The FNR gene of cultivar M36001 has an insertion in the coding region. Sorghums with a tan injury response have lower levels of flavan-4-ols (precursors of apigeninidin and luteolinidin-apiforol and luteoforol, respectively) than do sorghums with a purple or red injury response and are unable to synthesize 3-deoxyanthocyanidins
Sorghum bicolor
physiological function
the enzyme is responsible for the synthesis of 3-deoxyanthocyanidins. The variation of purple-red-orange is explained by the balance of two 3-deoxyanthocyanidins, uteolinidin and apigeninidin. The balance is controlled by the expression level of flavonoid 3'-hydroxylase (F3'H), which encodes a protein that hydroxylates the 3' position of the B-ring of naringenin to produce a precursor of luteolinidin
Sorghum bicolor
General Information (protein specific)
General Information
Commentary
Organism
malfunction
Sorghums with a tan injury response have non-functional FNR alleles (p). The FNR gene of cultivar M36001 has an insertion in the coding region. Sorghums with a tan injury response have lower levels of flavan-4-ols (precursors of apigeninidin and luteolinidin-apiforol and luteoforol, respectively) than do sorghums with a purple or red injury response and are unable to synthesize 3-deoxyanthocyanidins
Sorghum bicolor
physiological function
the enzyme is responsible for the synthesis of 3-deoxyanthocyanidins. The variation of purple-red-orange is explained by the balance of two 3-deoxyanthocyanidins, uteolinidin and apigeninidin. The balance is controlled by the expression level of flavonoid 3'-hydroxylase (F3'H), which encodes a protein that hydroxylates the 3' position of the B-ring of naringenin to produce a precursor of luteolinidin
Sorghum bicolor
Expression (protein specific)
Organism
Commentary
Expression
Sorghum bicolor
the enzyme is highly induced by cutting stress
up
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740494
Mizuno
Expression of flavone synthase ...
Sorghum bicolor
Front. Plant Sci.
7
1718
2016
-
-
1
-
1
-
-
-
-
-
-
3
-
2
-
-
-
-
-
1
-
-
4
-
3
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
740500
Kawahigashi
The sorghum gene for leaf colo ...
Sorghum bicolor
G3 (Bethesda)
6
1439-1447
2016
-
-
1
-
2
-
-
-
-
-
2
1
-
3
-
-
1
-
-
2
-
-
4
1
4
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
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-
2
1
-
-
-
1
-
2
-
-
4
1
1
-
-
-
1
-
-
-
1
3
3
1
-
-
725929
Li
Molecular characterization and ...
Saussurea medusa
Mol. Biol. Rep.
39
2991-2999
2012
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1
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-
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1
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5
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5
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2
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1
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1
1
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1
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5
-
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2
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-
-
-
-
-
-
-
3
3
-
-
-
654357
Fischer
Molecular cloning, substrate s ...
Malus domestica, Pyrus communis
Arch. Biochem. Biophys.
412
223-230
2003
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1
-
-
-
-
-
-
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6
-
6
-
-
-
2
-
2
-
-
10
-
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4
-
-
-
2
-
-
2
-
-
-
-
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1
2
-
-
-
-
-
-
-
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-
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6
-
-
-
-
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2
-
-
10
-
4
-
-
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2
-
-
-
-
-
-
-
-
-
657102
Halbwirth
-
Biochemical formation of antho ...
Zea mays
Plant Sci.
164
489-495
2003
-
-
1
-
-
-
2
-
-
-
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3
-
1
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-
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1
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1
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7
-
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1
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1
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2
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1
2
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-
2
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3
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1
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7
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1
-
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1
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-
-
-
-
-
286346
Stich
-
Biosynthesis of 3-deoxyanthocy ...
Sinningia cardinalis
Biochemistry
27
785-789
1988
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1
5
1
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1
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1
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1
1
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5
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1
1
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3
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3
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1
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5
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1
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1
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1
1
-
5
-
-
-
-
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1
1
-
-
-
-
-
-
-
-
438999
Stich
-
Studies on columnidin biosynth ...
Columnea hybrida, Columnea hybrida Heklua
Z. Naturforsch. C
43
311-314
1988
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1
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2
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2
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1
1
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6
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1
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1
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1
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1
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2
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1
1
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6
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1
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