BRENDA - Reference to 1.1.1.234; Id = 286346

freezing fresh flowers in liquid nitrogen and storing them at -80°C enhances extractable activity compared to fresh flowers

Sinningia cardinalis

glycerol

10% v/v, complete irreversible inactivation

Sinningia cardinalis

additional information

no inhibition by KCN, EDTA, diethyl dithiocarbamate, phenanthroline or N-ethylmaleimide, 2 mM each

Sinningia cardinalis

naringenin

above 0.1 mM

Sinningia cardinalis

p-chloromercuribenzoate

0.5 mM

Sinningia cardinalis

sucrose

20% w/v, complete irreversible inactivation

Sinningia cardinalis

0.021

0.023

(2S)-naringenin

-

Sinningia cardinalis

(2S)-flavanone + NADPH

Sinningia cardinalis

involved in the biosynthesis of 3-deoxyanthocyanidines such as apigeninidin or luteolinidin, from (2S)-flavanones naringenin or eriodictyol

(2S)-flavan-4-ol + NADP+

-

Sinningia cardinalis

?

Sinningia cardinalis

-

syn. Rechsteineria cardinalis, strain " Feuerschein"

-

flower

-

Sinningia cardinalis

-

0.015

-

Sinningia cardinalis

(2S)-eriodictyol + NADPH

-

286346

Sinningia cardinalis

luteoforol + NADP+

-

286346

Sinningia cardinalis

?

(2S)-flavanone + NADPH

involved in the biosynthesis of 3-deoxyanthocyanidines such as apigeninidin or luteolinidin, from (2S)-flavanones naringenin or eriodictyol

286346

Sinningia cardinalis

(2S)-flavan-4-ol + NADP+

-

286346

Sinningia cardinalis

?

(2S)-naringenin + NADPH + H+

-

286346

Sinningia cardinalis

apiforol + NADP+

-

286346

Sinningia cardinalis

?

5,7,3',4',5'-pentahydroxyflavanone + NADPH

-

286346

Sinningia cardinalis

5,7,3',4',5'-pentahydroxyflavan-4-ol + NADP+

-

286346

Sinningia cardinalis

?

additional information

as dihydroxyflavonols, such as dihydrokaempferol, dihydroquercetin and dihydromyricetin are substrates for the Sinningia enzyme preparation, too, the enzyme might be identical with dihydroflavonol-4-reductase

286346

Sinningia cardinalis

?

-

?

6

-

Sinningia cardinalis

4.7

6.9

about half-maximal activity at pH 4.7 and 6.9

Sinningia cardinalis

additional information

no cofactors are FAD, FMN or tetrahydropterin, with or without NADPH

Sinningia cardinalis

NADH

77% as active as NADPH

Sinningia cardinalis

NADPH

required

Sinningia cardinalis

additional information

no cofactors are FAD, FMN or tetrahydropterin, with or without NADPH

Sinningia cardinalis

NADH

77% as active as NADPH

Sinningia cardinalis

NADPH

required

Sinningia cardinalis

freezing fresh flowers in liquid nitrogen and storing them at -80°C enhances extractable activity compared to fresh flowers

Sinningia cardinalis

glycerol

10% v/v, complete irreversible inactivation

Sinningia cardinalis

additional information

no inhibition by KCN, EDTA, diethyl dithiocarbamate, phenanthroline or N-ethylmaleimide, 2 mM each

Sinningia cardinalis

naringenin

above 0.1 mM

Sinningia cardinalis

p-chloromercuribenzoate

0.5 mM

Sinningia cardinalis

sucrose

20% w/v, complete irreversible inactivation

Sinningia cardinalis

0.021

0.023

(2S)-naringenin

-

Sinningia cardinalis

(2S)-flavanone + NADPH

Sinningia cardinalis

involved in the biosynthesis of 3-deoxyanthocyanidines such as apigeninidin or luteolinidin, from (2S)-flavanones naringenin or eriodictyol

(2S)-flavan-4-ol + NADP+

-

Sinningia cardinalis

?

flower

-

Sinningia cardinalis

-

0.015

-

Sinningia cardinalis

(2S)-eriodictyol + NADPH

-

286346

Sinningia cardinalis

luteoforol + NADP+

-

286346

Sinningia cardinalis

?

(2S)-flavanone + NADPH

involved in the biosynthesis of 3-deoxyanthocyanidines such as apigeninidin or luteolinidin, from (2S)-flavanones naringenin or eriodictyol

286346

Sinningia cardinalis

(2S)-flavan-4-ol + NADP+

-

286346

Sinningia cardinalis

?

(2S)-naringenin + NADPH + H+

-

286346

Sinningia cardinalis

apiforol + NADP+

-

286346

Sinningia cardinalis

?

5,7,3',4',5'-pentahydroxyflavanone + NADPH

-

286346

Sinningia cardinalis

5,7,3',4',5'-pentahydroxyflavan-4-ol + NADP+

-

286346

Sinningia cardinalis

?

additional information

as dihydroxyflavonols, such as dihydrokaempferol, dihydroquercetin and dihydromyricetin are substrates for the Sinningia enzyme preparation, too, the enzyme might be identical with dihydroflavonol-4-reductase

286346

Sinningia cardinalis

?

-

?

6

-

Sinningia cardinalis

4.7

6.9

about half-maximal activity at pH 4.7 and 6.9

Sinningia cardinalis

740494

Mizuno

Expression of flavone synthase ...

Sorghum bicolor

Front. Plant Sci.

7

1718

2016

-

1

-

1

-

3

-

2

-

1

-

4

-

3

-

1

-

1

-

1

-

3

-

1

-

4

-

1

2

1

-

740500

Kawahigashi

The sorghum gene for leaf colo ...

Sorghum bicolor

G3 (Bethesda)

6

1439-1447

2016

-

1

-

2

-

2

1

-

4

-

1

-

2

-

4

1

4

1

-

1

-

1

-

1

-

2

-

2

1

-

1

-

2

-

4

1

-

1

-

1

3

1

-

725929

Li

Molecular characterization and ...

Saussurea medusa

Mol. Biol. Rep.

39

2991-2999

2012

-

1

-

1

-

5

-

5

-

2

-

1

-

1

-

1

-

5

-

2

-

3

-

654357

Fischer

Molecular cloning, substrate s ...

Pyrus communis, Malus domestica

Arch. Biochem. Biophys.

412

223-230

2003

-

1

-

6

-

6

-

2

-

2

-

10

-

4

-

2

-

2

-

1

2

-

6

-

2

-

10

-

4

-

2

-

657102

Halbwirth

-

Biochemical formation of antho ...

Zea mays

Plant Sci.

164

489-495

2003

-

1

-

2

-

3

-

1

-

1

-

1

-

7

-

1

-

1

-

2

-

1

2

-

2

-

3

-

1

-

7

-

1

-

1

-

286346

Stich

-

Biosynthesis of 3-deoxyanthocy ...

Sinningia cardinalis

Biochemistry

27

785-789

1988

-

1

5

1

-

1

-

1

-

1

-

5

-

1

-

3

-

3

-

1

-

5

-

1

-

1

-

1

-

5

-

1

-

438999

Stich

-

Studies on columnidin biosynth ...

Columnea hybrida, Columnea hybrida Heklua

Z. Naturforsch. C

43

311-314

1988

-

1

-

2

-

2

-

1

-

6

-

1

-

1

-

1

-

1

-

2

-

1

-

6

-

1

-

Biosynthesis of 3-deoxyanthocyanins with flower extracts from Sinningia cardinalis

Data extracted from this reference: