Cloned (Comment) | Organism |
---|---|
gene hisD, expression as glutathione S-transferase fusion protein with a thrombin cleavage site between tag and enzyme | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
15.4 mg/ml purified recombinant detagged enzyme alone or complexed with L-histidinol, L-histamine, or L-histidine or Zn2+ and NAD+, in 20 mM Tris-HCl, pH 7.5, 0.2 M NaCl, 5 mM DTT, 1 mM ligand, hanging drop vapour diffusion method, 18°C, 0.002 ml protein solution with 0.004 ml reservoir solution, containing 20% w/v PEG 3350, 7% v/v glycerol, 0.1 M imidazole-malic acid, pH 5.5, 0.2 M ammonium sulfate, 2 weeks, macroseeding for larger crystals, transfer to sodium acetate, pH 5.5, to eliminate the inhibiting imidazole, X-ray diffraction structure determination and analysis at 2.2 A resolution, modelling | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | binding site structure | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-histidinol + 2 NAD+ + H2O | Escherichia coli | part of L-histidine biosynthesis | L-histidine + 2 NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P06988 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme fusion protein | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-histidinol + 2 NAD+ + H2O = L-histidine + 2 NADH + 2 H+ | reaction mechanism, amino acid residues Glu326 and His327 are involved in catalysis | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-histidinol + 2 NAD+ + H2O | part of L-histidine biosynthesis | Escherichia coli | L-histidine + 2 NADH + 2 H+ | - |
? | |
L-histidinol + NAD+ | - |
Escherichia coli | L-histidinal + NADH + H+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | dynamic light scattering | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
HisD | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | binding site structure | Escherichia coli |