Purification and properties of an asymmetric reduction of diethyl 2-methyl-3-oxosuccinate in Saccharomyces fermentati
Furuichi, A.; Akita, H.; Matsukura, H.; Oishi, T.; Horikoshi, K.; Agric. Biol. Chem. 51, 293-299 (1987)
No PubMed abstract available
Data extracted from this reference:
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
30% of activity at 10 mM
Torulaspora delbrueckii
cysteine
complete inhibition at 10 mM
Torulaspora delbrueckii
FMN
slight inhibition at 0.2 mM
Torulaspora delbrueckii
high salt concentration
1M NaCl or KCl
Torulaspora delbrueckii
KCN
complete inhibition at 10 mM
Torulaspora delbrueckii
monoiodoacetate
complete inhibition at 10 mM
Torulaspora delbrueckii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.25
-
Diethyl 2-methyl-3-oxosuccinate
-
Torulaspora delbrueckii
111
-
Diethyl 2-methyl-3-hydroxysuccinate
-
Torulaspora delbrueckii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ag+
strong inhibitor
Torulaspora delbrueckii
Cu2+
strong inhibitor
Torulaspora delbrueckii
Fe3+
strong inhibitor
Torulaspora delbrueckii
K+
strongly inhibitory at 1 M
Torulaspora delbrueckii
Na+
strongly inhibitory at 1 M
Torulaspora delbrueckii
Zn2+
slight inhibition
Torulaspora delbrueckii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
61000
-
gel filtration
Torulaspora delbrueckii
63000
-
SDS-PAGE
Torulaspora delbrueckii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
diethyl 2-methyl-3-oxosuccinate + NADPH
Torulaspora delbrueckii
asymmetric reduction in yeast, the reduction is strongly preferred over the weak oxidation activity
diethyl 2-methyl-3-hydroxysuccinate + NADP+
-
-
r
Organism
Organism
UniProt
Commentary
Textmining
Torulaspora delbrueckii
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Torulaspora delbrueckii
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.004
-
crude extract
Torulaspora delbrueckii
0.649
-
purified enzyme
Torulaspora delbrueckii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
diethyl 2-methyl-3-oxosuccinate + NADPH
the reduction is strongly preferred, the catalyzed reaction is an asymmetric reduction, best substrate
389504
Torulaspora delbrueckii
diethyl 2-methyl-3-hydroxysuccinate + NADP+
alpha-methyl-beta-hydroxy ester, the enzyme catalyzes an asymmetric reduction, the product is a mixture of the (2R,3R)-syn- and (2S,3R)-anti-beta-hydroxyester
389504
Torulaspora delbrueckii
r
diethyl 2-methyl-3-oxosuccinate + NADPH
asymmetric reduction in yeast, the reduction is strongly preferred over the weak oxidation activity
389504
Torulaspora delbrueckii
diethyl 2-methyl-3-hydroxysuccinate + NADP+
-
-
-
r
dimethyl 2-methyl-3-oxosuccinate + NADPH
poor substrate
389504
Torulaspora delbrueckii
dimethyl 2-methyl-3-hydroxysuccinate + NADP+
-
-
-
?
additional information
no aldehydes or several synthetic substrates are reduced, such as: acetaldehyde, formaldehyde, isobutanal, n-pentanal, n-hexanal, n-heptanal, 4-hydroxybenzaldehyde, 4-dimethylaminobenzaldehyde, benzaldehyde, furfural, 2-methyl-3-oxobutanoic acid benzyl ester, 2-chloro-3-oxobutanoic acid ethyl ester, 3-phenyl-2-oxobutanoic acid methyl ester, 2-methyl-3-oxobutanoic acid ethyl ester, 3-(2'-furyl)-2-methyl-3-oxopropionic acid methyl ester, none of the following alcohols or other substrates is oxidized: methanol, ethanol, n-propanol, isopropanol, n-butanol, isobutanol, n-pentanol, isopentanol, benzyl alcohol, glycerin, shikimic acid, 2-deoxy-D-glucose, glucose 6-phosphate, in the presence of NAD+ or NADP+
389504
Torulaspora delbrueckii
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
-
Torulaspora delbrueckii
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Torulaspora delbrueckii
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
60
more than 70% of maximal activity at both temperatures
Torulaspora delbrueckii
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
very unstable at temperatures above 40°C for 10 min
Torulaspora delbrueckii
60
-
complete loss of activity
Torulaspora delbrueckii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
sharp peak
Torulaspora delbrueckii
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
8
less than 10% of activity at both pH
Torulaspora delbrueckii
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
9
stable for 30 min
Torulaspora delbrueckii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
30% of activity at 10 mM
Torulaspora delbrueckii
cysteine
complete inhibition at 10 mM
Torulaspora delbrueckii
FMN
slight inhibition at 0.2 mM
Torulaspora delbrueckii
high salt concentration
1M NaCl or KCl
Torulaspora delbrueckii
KCN
complete inhibition at 10 mM
Torulaspora delbrueckii
monoiodoacetate
complete inhibition at 10 mM
Torulaspora delbrueckii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.25
-
Diethyl 2-methyl-3-oxosuccinate
-
Torulaspora delbrueckii
111
-
Diethyl 2-methyl-3-hydroxysuccinate
-
Torulaspora delbrueckii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ag+
strong inhibitor
Torulaspora delbrueckii
Cu2+
strong inhibitor
Torulaspora delbrueckii
Fe3+
strong inhibitor
Torulaspora delbrueckii
K+
strongly inhibitory at 1 M
Torulaspora delbrueckii
Na+
strongly inhibitory at 1 M
Torulaspora delbrueckii
Zn2+
slight inhibition
Torulaspora delbrueckii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
61000
-
gel filtration
Torulaspora delbrueckii
63000
-
SDS-PAGE
Torulaspora delbrueckii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
diethyl 2-methyl-3-oxosuccinate + NADPH
Torulaspora delbrueckii
asymmetric reduction in yeast, the reduction is strongly preferred over the weak oxidation activity
diethyl 2-methyl-3-hydroxysuccinate + NADP+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Torulaspora delbrueckii
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.004
-
crude extract
Torulaspora delbrueckii
0.649
-
purified enzyme
Torulaspora delbrueckii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
diethyl 2-methyl-3-oxosuccinate + NADPH
the reduction is strongly preferred, the catalyzed reaction is an asymmetric reduction, best substrate
389504
Torulaspora delbrueckii
diethyl 2-methyl-3-hydroxysuccinate + NADP+
alpha-methyl-beta-hydroxy ester, the enzyme catalyzes an asymmetric reduction, the product is a mixture of the (2R,3R)-syn- and (2S,3R)-anti-beta-hydroxyester
389504
Torulaspora delbrueckii
r
diethyl 2-methyl-3-oxosuccinate + NADPH
asymmetric reduction in yeast, the reduction is strongly preferred over the weak oxidation activity
389504
Torulaspora delbrueckii
diethyl 2-methyl-3-hydroxysuccinate + NADP+
-
-
-
r
dimethyl 2-methyl-3-oxosuccinate + NADPH
poor substrate
389504
Torulaspora delbrueckii
dimethyl 2-methyl-3-hydroxysuccinate + NADP+
-
-
-
?
additional information
no aldehydes or several synthetic substrates are reduced, such as: acetaldehyde, formaldehyde, isobutanal, n-pentanal, n-hexanal, n-heptanal, 4-hydroxybenzaldehyde, 4-dimethylaminobenzaldehyde, benzaldehyde, furfural, 2-methyl-3-oxobutanoic acid benzyl ester, 2-chloro-3-oxobutanoic acid ethyl ester, 3-phenyl-2-oxobutanoic acid methyl ester, 2-methyl-3-oxobutanoic acid ethyl ester, 3-(2'-furyl)-2-methyl-3-oxopropionic acid methyl ester, none of the following alcohols or other substrates is oxidized: methanol, ethanol, n-propanol, isopropanol, n-butanol, isobutanol, n-pentanol, isopentanol, benzyl alcohol, glycerin, shikimic acid, 2-deoxy-D-glucose, glucose 6-phosphate, in the presence of NAD+ or NADP+
389504
Torulaspora delbrueckii
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
-
Torulaspora delbrueckii
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Torulaspora delbrueckii
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
60
more than 70% of maximal activity at both temperatures
Torulaspora delbrueckii
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
very unstable at temperatures above 40°C for 10 min
Torulaspora delbrueckii
60
-
complete loss of activity
Torulaspora delbrueckii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
sharp peak
Torulaspora delbrueckii
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
8
less than 10% of activity at both pH
Torulaspora delbrueckii
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
9
stable for 30 min
Torulaspora delbrueckii
Other publictions for EC 1.1.1.229
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
389504
Furuichi
-
Purification and properties of ...
Torulaspora delbrueckii
Agric. Biol. Chem.
51
293-299
1987
-
-
-
-
-
-
6
2
-
6
2
1
-
1
-
-
1
-
-
-
2
-
4
1
-
1
1
2
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
6
-
2
-
6
2
1
-
-
-
1
-
-
2
-
4
1
1
1
2
-
1
1
1
-
-
-
-
-
-
-