Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.223 extracted from

  • Kjonaas, R.B.; Venkatachalam, K.V.; Croteau, R.
    Metabolism of monoterpenes: oxidation of isopiperitenol to isopiperitenone, and subsequent isomerization to piperitenone by soluble enzyme preparations from peppermint (Mentha piperita) leaves (1985), Arch. Biochem. Biophys., 238, 49-60.
    View publication on PubMed

General Stability

General Stability Organism
stabilized with bovine serum albumin Mentha x piperita

Inhibitors

Inhibitors Comment Organism Structure
iodacetamide slight inhibition Mentha x piperita
N-ethylmaleimide slight inhibition Mentha x piperita
p-hydroxymercuribenzoate complete inhibition at 0.025 nM Mentha x piperita

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
NAD+
-
Mentha x piperita
0.02
-
(+)-trans-isoperitenol
-
Mentha x piperita
0.02
-
(-)-trans-isoperitenol
-
Mentha x piperita
0.08
-
(-)-cis-isoperitenol
-
Mentha x piperita
0.08
-
(+)-cis-isopiperitenol
-
Mentha x piperita
2.5
-
NADP+
-
Mentha x piperita

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Mentha x piperita
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Hg2+ complete inhibition at 0.1 mM Mentha x piperita

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
gel filtration Mentha x piperita

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(-)-trans-isopiperitenol + NAD+ Mentha x piperita central enzyme in menthol biosynthesis pathway (-)-isopiperitenone + NADH
-
r

Organism

Organism UniProt Comment Textmining
Mentha x piperita
-
peppermint
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Mentha x piperita
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-cis-isopiperitenol + NAD+
-
Mentha x piperita (+)-isopiperitenone + NADH + H+
-
r
(+)-isomenthol + NAD+ activity less than 2% of that of (-)-trans-isopiperitenol Mentha x piperita (+)-isomenthone + NADH
-
?
(+)-trans-isopiperitenol + NAD+ 95% activity of that of (-)-trans-isopiperitenol Mentha x piperita (+)-isopiperitenone + NADH
-
?
(+)-trans-pulegol + NAD+ 19% activity of that of (-)-trans-isopiperitenol Mentha x piperita (R)-pulegone + NADH
-
?
(+,-)-cis-isopiperitenol + NAD+
-
Mentha x piperita (+,-)-isopiperitenone
-
?
(-)-trans-isopiperitenol + NAD+ preferred substrate, not readily reversible in pH range 6.5-10.5 Mentha x piperita (-)-isopiperitenone + NADH
-
r
(-)-trans-isopiperitenol + NAD+ central enzyme in menthol biosynthesis pathway Mentha x piperita (-)-isopiperitenone + NADH
-
r
(-)-trans-isopiperitenol + NADP+
-
Mentha x piperita (-)-isopiperitenone + NADPH + H+
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 10.5
-
Mentha x piperita

pH Range

pH Minimum pH Maximum Comment Organism
7.5 11 half maximum activity at both pH Mentha x piperita

Cofactor

Cofactor Comment Organism Structure
additional information FAD completely ineffective Mentha x piperita
NAD+
-
Mentha x piperita
NADP+ less than 10% of the activity with NAD Mentha x piperita