General Stability | Organism |
---|---|
stabilized with bovine serum albumin | Mentha x piperita |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodacetamide | slight inhibition | Mentha x piperita | |
N-ethylmaleimide | slight inhibition | Mentha x piperita | |
p-hydroxymercuribenzoate | complete inhibition at 0.025 nM | Mentha x piperita |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
NAD+ | - |
Mentha x piperita | |
0.02 | - |
(+)-trans-isoperitenol | - |
Mentha x piperita | |
0.02 | - |
(-)-trans-isoperitenol | - |
Mentha x piperita | |
0.08 | - |
(-)-cis-isoperitenol | - |
Mentha x piperita | |
0.08 | - |
(+)-cis-isopiperitenol | - |
Mentha x piperita | |
2.5 | - |
NADP+ | - |
Mentha x piperita |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Mentha x piperita | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | complete inhibition at 0.1 mM | Mentha x piperita |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
gel filtration | Mentha x piperita |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-trans-isopiperitenol + NAD+ | Mentha x piperita | central enzyme in menthol biosynthesis pathway | (-)-isopiperitenone + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mentha x piperita | - |
peppermint | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Mentha x piperita | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-cis-isopiperitenol + NAD+ | - |
Mentha x piperita | (+)-isopiperitenone + NADH + H+ | - |
r | |
(+)-isomenthol + NAD+ | activity less than 2% of that of (-)-trans-isopiperitenol | Mentha x piperita | (+)-isomenthone + NADH | - |
? | |
(+)-trans-isopiperitenol + NAD+ | 95% activity of that of (-)-trans-isopiperitenol | Mentha x piperita | (+)-isopiperitenone + NADH | - |
? | |
(+)-trans-pulegol + NAD+ | 19% activity of that of (-)-trans-isopiperitenol | Mentha x piperita | (R)-pulegone + NADH | - |
? | |
(+,-)-cis-isopiperitenol + NAD+ | - |
Mentha x piperita | (+,-)-isopiperitenone | - |
? | |
(-)-trans-isopiperitenol + NAD+ | preferred substrate, not readily reversible in pH range 6.5-10.5 | Mentha x piperita | (-)-isopiperitenone + NADH | - |
r | |
(-)-trans-isopiperitenol + NAD+ | central enzyme in menthol biosynthesis pathway | Mentha x piperita | (-)-isopiperitenone + NADH | - |
r | |
(-)-trans-isopiperitenol + NADP+ | - |
Mentha x piperita | (-)-isopiperitenone + NADPH + H+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 10.5 | - |
Mentha x piperita |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 11 | half maximum activity at both pH | Mentha x piperita |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | FAD completely ineffective | Mentha x piperita | |
NAD+ | - |
Mentha x piperita | |
NADP+ | less than 10% of the activity with NAD | Mentha x piperita |