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Literature summary for 1.1.1.22 extracted from

  • Beattie, N.R.; Pioso, B.J.; Sidlo, A.M.; Keul, N.D.; Wood, Z.A.
    Hysteresis and allostery in human UDP-glucose dehydrogenase require a flexible protein core (2018), Biochemistry, 57, 6848-6859 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.22

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant A104L, structure reveals a reduced cavity C-1 volume. The allosteric switch still adopts the E and E* states, albeit with a more rigid protein core Homo sapiens

Protein Variants

Protein Variants Comment Organism
A104L ubstitution introduced to fill a cavity in the E state and sterically prevent repacking of the core into the inactive Eomega state. Mutant A104L does not show hysteresis or negative cooperativity, binds UDP-xylose with lower affinity and the inhibition is no longer cooperative Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0076
-
 UDP-alpha-D-glucose wild-type, K0.5 value, Hill coefficient 1, pH 7.5, 25°C Homo sapiens
0.0094
-
 UDP-alpha-D-glucose mutant A104L, K0.5 value, Hill coefficient 1, pH 7.5, 25°C Homo sapiens
0.048
-
 NAD+ mutant A104L, K0.5 value, Hill coefficient 1, pH 7.5, 25°C Homo sapiens
1.16
-
 NAD+ wild-type, K0.5 value, Hill coefficient 0.69, pH 7.5, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O60701
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + 2 NAD+ + H2O
-
 Homo sapiens UDP-alpha-D-glucuronate + 2 NADH + 2 H+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.59
-
 UDP-alpha-D-glucose wild-type, pH 7.5, 25°C Homo sapiens
0.79
-
 NAD+ mutant A104L, pH 7.5, 25°C Homo sapiens
0.82
-
 UDP-alpha-D-glucose mutant A104L, pH 7.5, 25°C Homo sapiens
0.94
-
 NAD+ wild-type, pH 7.5, 25°C Homo sapiens