Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
mutant A136M at 2.05 A resolution, the A136M substitution has stabilized the active conformation of the T131-loop/alpha6 allosteric switch | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A136M | mutant does not exhibit substrate cooperativity. The inhibitor affinity of A136M is reduced 14fold and does not exhibit hysteresis. Substitution disrupts NAD+-induced negative cooperativity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00726 | - |
UDP-alpha-D-glucose | mutant A136M, Hill coefficient, pH 7.5, 25°C | Homo sapiens | |
0.0097 | - |
UDP-alpha-D-glucose | wild-type, Hill coefficient 1, pH 7.5, 25°C | Homo sapiens | |
0.09 | - |
NAD+ | mutant A136M, Hill coefficient, pH 7.5, 25°C | Homo sapiens | |
0.78 | - |
NAD+ | wild-type, Hill coefficient 0.73, pH 7.5, 25°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O60701 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + 2 NAD+ + H2O | - |
Homo sapiens | UDP-alpha-D-glucuronate + 2 NADH + 2 H+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.55 | - |
UDP-alpha-D-glucose | wild-type, pH 7.5, 25°C | Homo sapiens | |
0.73 | - |
UDP-alpha-D-glucose | mutant A136M, pH 7.5, 25°C | Homo sapiens | |
0.76 | - |
NAD+ | wild-type, pH 7.5, 25°C | Homo sapiens | |
0.83 | - |
NAD+ | mutant A136M, pH 7.5, 25°C | Homo sapiens |