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Literature summary for 1.1.1.22 extracted from

  • Beattie, N.; Keul, N.; Sidlo, A.; Wood, Z.
    Allostery and hysteresis are coupled in human UDP-glucose dehydrogenase (2017), Biochemistry, 56, 202-211 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant A136M at 2.05 A resolution, the A136M substitution has stabilized the active conformation of the T131-loop/alpha6 allosteric switch Homo sapiens

Protein Variants

Protein Variants Comment Organism
A136M mutant does not exhibit substrate cooperativity. The inhibitor affinity of A136M is reduced 14fold and does not exhibit hysteresis. Substitution disrupts NAD+-induced negative cooperativity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00726
-
UDP-alpha-D-glucose mutant A136M, Hill coefficient, pH 7.5, 25°C Homo sapiens
0.0097
-
UDP-alpha-D-glucose wild-type, Hill coefficient 1, pH 7.5, 25°C Homo sapiens
0.09
-
NAD+ mutant A136M, Hill coefficient, pH 7.5, 25°C Homo sapiens
0.78
-
NAD+ wild-type, Hill coefficient 0.73, pH 7.5, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O60701
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + 2 NAD+ + H2O
-
Homo sapiens UDP-alpha-D-glucuronate + 2 NADH + 2 H+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.55
-
UDP-alpha-D-glucose wild-type, pH 7.5, 25°C Homo sapiens
0.73
-
UDP-alpha-D-glucose mutant A136M, pH 7.5, 25°C Homo sapiens
0.76
-
NAD+ wild-type, pH 7.5, 25°C Homo sapiens
0.83
-
NAD+ mutant A136M, pH 7.5, 25°C Homo sapiens