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Literature summary for 1.1.1.22 extracted from

  • Hwang, E.Y.; Huh, J.W.; Choi, M.M.; Choi, S.Y.; Hong, H.N.; Cho, S.W.
    Inhibitory effects of gallic acid and quercetin on UDP-glucose dehydrogenase activity (2008), FEBS Lett., 582, 3793-3797.
    View publication on PubMed
Search for more literature for 1.1.1.22

Application

Application Comment Organism
medicine possibility that gallic acid and quercetin may modulate human breast cancer cell proliferation by inhibiting UGDH Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
gallic acid is a non-competitive inhibitor with respect to UDP-glucose and NAD+. It decreases specific activities of UGDH, but does not affect UGDH protein expression, thus UGDH activity is inhibited by polyphenols at the post-translational level. Gallic acid exerts strong antiproliferative activity in breast cancer cells. Heat inactivation of UGDH is accelerated to a greater degree by quercetin than by gallic acid. In the presence of gallic acid, the activity remaining after 30 min is 55% that of control Homo sapiens
quercetin shows a competitive inhibition and a mixed-type inhibition with respect to UDP-glucose and NAD+, decreases specific activities of UGDH, but does not affect UGDH protein expression, thus UGDH activity is inhibited by polyphenols at the post-translational level. Quercetin exerts strong antiproliferative activity in breast cancer cells. Heat inactivation of UGDH is accelerated to a greater degree by quercetin than by gallic acid. In the presence of quercetin, the activity remaining after 30 min is 20% that of control Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 UDP-glucose
-
 Homo sapiens
0.13
-
 NAD+
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
MCF-7 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00051
-
 lysates of cells treated with 0.3 mM gallic acid Homo sapiens
0.00061
-
 lysates of cells treated with 0.3 mM quercetin Homo sapiens
0.0015
-
 control lysates Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + 2 NAD+ + H2O
-
 Homo sapiens UDP-glucuronate + 2 NADH + 2 H+
-
 ?

Synonyms

Synonyms Comment Organism
UDP-glucose dehydrogenase
-
 Homo sapiens
UGDH
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.07
-
 quercetin with respect to NAD+ Homo sapiens
0.083
-
 quercetin with respect to UDP-glucose Homo sapiens
0.744
-
 gallic acid with respect to UDP-glucose Homo sapiens
1.804
-
 gallic acid with respect to NAD+ Homo sapiens