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Literature summary for 1.1.1.219 extracted from

  • Mei, X.; Zhou, C.; Zhang, W.; Rothenberg, D.O.; Wan, S.; Zhang, L.
    Comprehensive analysis of putative dihydroflavonol 4-reductase gene family in tea plant (2019), PLoS ONE, 14, e0227225 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.219

Protein Variants

Protein Variants Comment Organism
N141A mutation modifies the preference of DFRa for dihydroquercetin and dihydromyricetin, and also weakens its stability Camellia sinensis
N141D mutation modifies the preference of DFRa for dihydroquercetin and dihydromyricetin, and also weakens its stability Camellia sinensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 (+)-dihydroquercetin wild-type, pH 7.2, 37°C Camellia sinensis
0.037
-
 (+)-dihydromyricetin mutant N141D, pH 7.2, 37°C Camellia sinensis
0.043
-
 (+)-dihydromyricetin wild-type, pH 7.2, 37°C Camellia sinensis
0.088
-
 (+)-dihydroquercetin mutant N141A, pH 7.2, 37°C Camellia sinensis
0.091
-
 (+)-dihydromyricetin mutant N141A, pH 7.2, 37°C Camellia sinensis
0.252
-
 (+)-dihydroquercetin mutant N141D, pH 7.2, 37°C Camellia sinensis
0.398
-
 (+)-dihydrokaempferol mutant N141A, pH 7.2, 37°C Camellia sinensis
0.539
-
 (+)-dihydrokaempferol wild-type, pH 7.2, 37°C Camellia sinensis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Camellia sinensis 5737
-

Organism

Organism UniProt Comment Textmining
Camellia sinensis Q9S787 isoform DFRa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-dihydrokaempferol + NADPH + H+
-
 Camellia sinensis cis-3,4-leucopelargonidin + NADP+
-
 ?
(+)-dihydromyricetin + NADPH + H+
-
 Camellia sinensis cis-3,4-leucodelphinidin + NADP+
-
 ?
(+)-dihydroquercetin + NADPH + H+
-
 Camellia sinensis cis-3,4-leucocyanidin + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, calculated from sequence Camellia sinensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 30 min, loss of activity Camellia sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Camellia sinensis

pH Range

pH Minimum pH Maximum Comment Organism
4
-
 less than 10% residual activity Camellia sinensis

pI Value

Organism Comment pI Value Maximum pI Value
Camellia sinensis calculated from sequence
-
 5.7