Cloned (Comment) | Organism |
---|---|
gene IbDFR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis | Ipomoea batatas |
Protein Variants | Comment | Organism |
---|---|---|
additional information | downregulation of IbDFR expression in transgenic sweet potato by RNAi approach | Ipomoea batatas |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ipomoea batatas | - |
cv. Ayamurasaki | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Ipomoea batatas | - |
hypocotyl | - |
Ipomoea batatas | - |
leaf | - |
Ipomoea batatas | - |
additional information | enzyme expression analysis in different tissues and anthocyanidins contents, overview | Ipomoea batatas | - |
root | - |
Ipomoea batatas | - |
seed coat | - |
Ipomoea batatas | - |
stem | - |
Ipomoea batatas | - |
Synonyms | Comment | Organism |
---|---|---|
DFR | - |
Ipomoea batatas |
dihydroflavonol-4-reductase | - |
Ipomoea batatas |
IbDFR | - |
Ipomoea batatas |
General Information | Comment | Organism |
---|---|---|
malfunction | downregulation of IbDFR expression in transgenic sweet potato (DFRi) using an RNAi approach dramatically reduces anthocyanin accumulation in young leaves, stems and storage roots. The increase of flavonols quercetin-3-O-hexose-hexoside and quercetin-3-O-glucoside in the leaves and roots of DFRi plants is significant. The metabolic pathway channels greater flavonol influx in the DFRi plants when their anthocyanin and proanthocyanidin accumulation are decreased. These plants also display reduced antioxidant capacity compared to the wild-type. After 24 h of cold treatment and 2 h recovery, the wild-type plants are almost fully restored to the initial phenotype compared to the slower recovery of DFRi plants, in which the levels of electrolyte leakage and hydrogen peroxide accumulation are dramatically increased | Ipomoea batatas |
metabolism | dihydroflavonol-4-reductase (DFR) is a key enzyme in the catalysis of the stereospecific reduction of dihydroflavonols to leucoanthocyanidins in anthocyanin biosynthesis | Ipomoea batatas |
physiological function | the enzyme expression is strongly associated with anthocyanin accumulation in leaves, stems and roots. The enzyme plays important roles in flavonoid metabolism, protective function of anthocyanins in enhanced scavenging of reactive oxygen radicals in plants under stressful conditions | Ipomoea batatas |