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Literature summary for 1.1.1.218 extracted from

  • Endo, S.; Noda, M.; Ikari, A.; Tatematsu, K.; El-Kabbani, O.; Hara, A.; Kitade, Y.; Matsunaga, T.
    Characterization of hamster NAD+-dependent 3(17)beta-hydroxysteroid dehydrogenase belonging to the aldo-keto reductase 1C subfamily (2015), J. Biochem., 158, 425-434.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene AKR1C34, cloned from liver, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS Mesocricetus auratus

Inhibitors

Inhibitors Comment Organism Structure
Hexestrol
-
Mesocricetus auratus
hinokitiol
-
Mesocricetus auratus
additional information no or poor inhibition by 7-hydroxyflavone, chrysin, quercetin, genistein, galangin, lithocholic acid, and zearalenone Mesocricetus auratus
Phenolphthalein
-
Mesocricetus auratus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
morphine + NAD+ Mesocricetus auratus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Mesocricetus auratus A0A097ZMY7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, ultrafiltration, and dialysis Mesocricetus auratus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland in male hamsters Mesocricetus auratus
-
colon in male hamsters Mesocricetus auratus
-
heart in female hamsters Mesocricetus auratus
-
kidney in male hamsters Mesocricetus auratus
-
liver
-
Mesocricetus auratus
-
additional information mRNA for AKR1C34 is expressed liver-specific in female hamsters and ubiquitously in male hamsters Mesocricetus auratus
-
small intestine in male hamsters Mesocricetus auratus
-
testis in male hamsters Mesocricetus auratus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme AKR1C34 shows 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase activities, EC 1.1.1.239, detailed overview. Ala54 plays a critical role in non-/recognition of the steroidal substrates. Reaction products identification by thin-layer chromatography (TLC) and liquid chromatography/mass spectrometry (LC/MS) Mesocricetus auratus ?
-
?
morphine + NAD+
-
Mesocricetus auratus ?
-
?
trans-naphthalene dihydrodiol + NAD+
-
Mesocricetus auratus ?
-
?

Synonyms

Synonyms Comment Organism
AKR1C34
-
Mesocricetus auratus
morphine-6-dehydrogenase UniProt Mesocricetus auratus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
assay at Mesocricetus auratus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Mesocricetus auratus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00065
-
pH 7.4, 25┬░C, recombinant enzyme Mesocricetus auratus Phenolphthalein
0.0013
-
pH 7.4, 25┬░C, recombinant enzyme Mesocricetus auratus Hexestrol
0.0032
-
pH 7.4, 25┬░C, recombinant enzyme Mesocricetus auratus hinokitiol

General Information

General Information Comment Organism
evolution enzyme M6DH is a member of the aldo┬ľketo reductase (AKR) 1C subfamily in the AKR superfamily Mesocricetus auratus