Cloned (Comment) | Organism |
---|---|
gene AKR1C34, cloned from liver, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS | Mesocricetus auratus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hexestrol | - |
Mesocricetus auratus | |
hinokitiol | - |
Mesocricetus auratus | |
additional information | no or poor inhibition by 7-hydroxyflavone, chrysin, quercetin, genistein, galangin, lithocholic acid, and zearalenone | Mesocricetus auratus | |
Phenolphthalein | - |
Mesocricetus auratus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
morphine + NAD+ | Mesocricetus auratus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesocricetus auratus | A0A097ZMY7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, ultrafiltration, and dialysis | Mesocricetus auratus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | in male hamsters | Mesocricetus auratus | - |
colon | in male hamsters | Mesocricetus auratus | - |
heart | in female hamsters | Mesocricetus auratus | - |
kidney | in male hamsters | Mesocricetus auratus | - |
liver | - |
Mesocricetus auratus | - |
additional information | mRNA for AKR1C34 is expressed liver-specific in female hamsters and ubiquitously in male hamsters | Mesocricetus auratus | - |
small intestine | in male hamsters | Mesocricetus auratus | - |
testis | in male hamsters | Mesocricetus auratus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme AKR1C34 shows 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase activities, EC 1.1.1.239, detailed overview. Ala54 plays a critical role in non-/recognition of the steroidal substrates. Reaction products identification by thin-layer chromatography (TLC) and liquid chromatography/mass spectrometry (LC/MS) | Mesocricetus auratus | ? | - |
? | |
morphine + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
trans-naphthalene dihydrodiol + NAD+ | - |
Mesocricetus auratus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AKR1C34 | - |
Mesocricetus auratus |
morphine-6-dehydrogenase | UniProt | Mesocricetus auratus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mesocricetus auratus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mesocricetus auratus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00065 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | Phenolphthalein | |
0.0013 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | Hexestrol | |
0.0032 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | hinokitiol |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme M6DH is a member of the aldoketo reductase (AKR) 1C subfamily in the AKR superfamily | Mesocricetus auratus |