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Literature summary for 1.1.1.218 extracted from

  • Todaka, T.; Yamano, S.; Toki, S.
    Purification and characterization of NAD-dependent morphine 6-dehydrogenase from hamster liver cytosol, a new member of the aldo-keto reductase superfamily (2000), Arch. Biochem. Biophys., 374, 189-197.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ketamine
-
Mesocricetus auratus
lithocholic acid moderate inhibitory effect Mesocricetus auratus
additional information competitive inhibitors: testosterone, 5alpha-androstan-17beta-ol-3-one, 5beta-androsteran-17beta-ol-3-one; no inhibition by indomethacine; no or weak inhibition by pyrazole, barbital Mesocricetus auratus
NEM
-
Mesocricetus auratus
p-chloromercuribenzoic acid
-
Mesocricetus auratus
Phenylarsine oxide
-
Mesocricetus auratus
quercetin weak inhibitory effect Mesocricetus auratus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
5alpha-androstan-17beta-ol-3-one similar values of other 17beta-hydroxysteroids Mesocricetus auratus
0.009
-
testosterone cosubstrate NAD+ Mesocricetus auratus
1.25
-
morphine cosubstrate NAD+ Mesocricetus auratus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Mesocricetus auratus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
native enzyme, gel filtration Mesocricetus auratus
38000
-
denatured enzyme, SDS-PAGE Mesocricetus auratus

Organism

Organism UniProt Comment Textmining
Mesocricetus auratus
-
Syrian hamster
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, various chromatographic techniques Mesocricetus auratus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Mesocricetus auratus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.21
-
morphine, NAD+ Mesocricetus auratus

Storage Stability

Storage Stability Organism
-80┬░C, 20% glycerol, v/v, stable at least four months Mesocricetus auratus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
codeine + NAD(P)+ only weak enzyme activity for codeine Mesocricetus auratus codeinone + NAD(P)H
-
?
morphine + NADP+ enzyme activity only with NAD+ Mesocricetus auratus morphinone + NADPH + H+
-
?
normorphine + NAD(P)+ best substrate, oxidation at 139% the rate of morphine oxidation Mesocricetus auratus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 38000, SDS-PAGE Mesocricetus auratus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
enzyme assay Mesocricetus auratus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.3
-
in glycine-NaCl-NaOH buffer Mesocricetus auratus

Cofactor

Cofactor Comment Organism Structure
NAD+ with NADP+ enzyme activity less than 5% those found with NAD+ Mesocricetus auratus