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Literature summary for 1.1.1.218 extracted from

  • Yamano, S.; Ito, K.; Ogata, S.; Toki, S.
    Purification, characterization and partial primary structure of morphine 6-dehydrogenase from rabbit liver cytosol (1997), Arch. Biochem. Biophys., 341, 81-88.
    View publication on PubMed

General Stability

General Stability Organism
enzyme stable without 2-mercaptoethanol Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
5beta-dihydrotestosterone
-
Oryctolagus cuniculus
additional information 5beta-dihydrotestosterone, lithocholic acid, androsterone and estradiol inhibit competitively toward morphine binding; little inhibitory effect: indomethacin, prostaglandins E2, D2; no or weak inhibition by pyrazole, barbital Oryctolagus cuniculus
NEM
-
Oryctolagus cuniculus
p-chloromercuribenzoic acid
-
Oryctolagus cuniculus
Phenylarsine oxide
-
Oryctolagus cuniculus
quercetin
-
Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
codeine with NAD+ Oryctolagus cuniculus
0.12
-
1-Indanol cosubstrate NAD+ Oryctolagus cuniculus
0.39
-
Dihydrocodeine cosubstrate NAD+ Oryctolagus cuniculus
0.54
-
cyclohexene-2-ol cosubstrate NAD+ Oryctolagus cuniculus
0.64
-
normorphine cosubstrate NAD+ Oryctolagus cuniculus
0.72
-
morphine cosubstrate NAD+ Oryctolagus cuniculus
0.92
-
3-hydroxyhexobarbital cosubstrate NAD+ Oryctolagus cuniculus
1.48
-
ethylmorphine cosubstrate NAD+ Oryctolagus cuniculus
1.75
-
benzenedihydrodiol cosubstrate NAD+ Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Oryctolagus cuniculus 5829
-
microsome microsomal fraction Oryctolagus cuniculus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
native enzyme, gel filtration Oryctolagus cuniculus
36000
-
denatured enzyme, SDS-PAGE Oryctolagus cuniculus
38000
-
1 * 38000, SDS-PAGE Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
Japanese white rabbit
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, various chromatographic techniques Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.43
-
morphine, NAD+ Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
-80┬░C, 20% glycerol, v/v, stable at least four months Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-indanol + NADP+
-
Oryctolagus cuniculus 1-indanone + NADPH
-
?
3-hydroxyhexobarbital + NADP+ does not serve as a substrate for guinea pig enzyme Oryctolagus cuniculus ?
-
?
benzenedihydrodiol + NADP+
-
Oryctolagus cuniculus ?
-
?
codeine + NAD(P)+ codeine best substrate Oryctolagus cuniculus codeinone + NAD(P)H
-
?
cyclohexene-2-ol + NADP+
-
Oryctolagus cuniculus cyclohexene-2-one + NADPH
-
?
dihydrocodeine + NADP+
-
Oryctolagus cuniculus dihydrocodeinone + NADPH
-
?
ethylmorphine + NAD(P)+
-
Oryctolagus cuniculus ?
-
?
morphine + NADP+ enzyme acitivity with NAD+ four times that with NADP+ Oryctolagus cuniculus morphinone + NADPH + H+
-
?
normorphine + NAD(P)+
-
Oryctolagus cuniculus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 38000, SDS-PAGE Oryctolagus cuniculus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
enzyme assay Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
with NADP+ as cofactor, in glycine-NaCl-NaOH buffer Oryctolagus cuniculus
9.4
-
with NAD+ as cofactor, in glycine-NaCl-NaOH buffer Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
NAD(P)+ NAD+ preferred cofactor Oryctolagus cuniculus