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Literature summary for 1.1.1.218 extracted from
- Guinea-pig liver morphine 6-dehydrogenase as a naloxone reductase (1986), Biochem. Pharmacol., 35, 4321-4326.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuSO4 | - |
Cavia porcellus |  |
| Ketamine | - |
Cavia porcellus | |
| lithocholic acid | - |
Cavia porcellus | |
| additional information | no or weak inhibition by pyrazole, barbital | Cavia porcellus | |
| Phenylarsine oxide | - |
Cavia porcellus | |
| prostaglandin E1 | - |
Cavia porcellus | |
| quercitrin | - |
Cavia porcellus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0022 | - |
NADH | - |
Cavia porcellus | |
| 0.0065 | - |
NADPH | - |
Cavia porcellus | |
| 0.27 | - |
naloxone | cosubstrate NADPH | Cavia porcellus | |
| 0.44 | - |
naloxone | cosubstrate NADH | Cavia porcellus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Cavia porcellus | 5829 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
| Cavia porcellus Hartley | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration, various chromatographic techniques | Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.86 | - |
morphine, NADP+ | Cavia porcellus |
| 0.9 | - |
naloxone, NADPH | Cavia porcellus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 2-4°C, 50 mM sodium phosphate buffer + 100 mM 2-mercaptoethanol, pH 6., 90% of the activity retained with both NAD+ and NADP+ for a week | Cavia porcellus |
| 4°C, 80% of the activity after a week in 50 mM sodium phosphate buffer, pH 6.0, 100 mM 2-mercaptoethanol, more stable at pH 6.0 than at pH 8.0 | Cavia porcellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| morphine + NADP+ | - |
Cavia porcellus | morphinone + NADPH + H+ | - |
? | |
| morphine + NADP+ | - |
Cavia porcellus Hartley | morphinone + NADPH + H+ | - |
? | |
| naloxone + NAD(P)H | - |
Cavia porcellus | 6alpha-naloxol + NADP+ | stereospecific reduction | ? | |
| naloxone + NAD(P)H | - |
Cavia porcellus Hartley | 6alpha-naloxol + NADP+ | stereospecific reduction | ? | |
| naloxone + NADH + H+ | - |
Cavia porcellus | ? | - |
r | |
| naloxone + NADH + H+ | - |
Cavia porcellus Hartley | ? | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| naloxone reductase | - |
Cavia porcellus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
enzyme assay, reduction of naloxone | Cavia porcellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
with NADH als cofactor, 67 mM sodium phosphate buffer | Cavia porcellus |
| 6.8 | - |
with NADPH as cofactor, 67 mM sodium phosphate buffer | Cavia porcellus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
at pH6.0 more stable than at pH 8.0 on storage at 4°C | Cavia porcellus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD(P)+ | utilized both with nearly equal efficiency | Cavia porcellus | |
| NAD(P)H | NADPH: 2.7 times as effective as NADH | Cavia porcellus | |
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