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Literature summary for 1.1.1.218 extracted from

  • Yamano, S; Kageura, E.; Ishida, T.; Toki, S.
    Purification and characterization of guinea pig liver morphine 6-dehydrogenase (1985), J. Biol. Chem., 260, 5259-5264.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol reversible Cavia porcellus

General Stability

General Stability Organism
2-mercaptoethanol stabilizes during purification and storage, removal inactivates, reversible Cavia porcellus

Inhibitors

Inhibitors Comment Organism Structure
CdCl2 dithiol modifier, strong competitive inhibitor to cofactor Cavia porcellus
additional information no inhibition by NEM, PCMB, iodoacetic acid Cavia porcellus
Phenylarsine oxide dithiol modifier, competitive inhibitor to cofactor binding and noncompetitively toward morphine binding Cavia porcellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
nalorphine cosubstrate NAD+ Cavia porcellus
0.033
-
codeine with NADP+ Cavia porcellus
0.044
-
normorphine cosubstrate NAD+ Cavia porcellus
0.048
-
nalorphine cosubstrate NADP+ Cavia porcellus
0.079
-
codeine with NAD+ Cavia porcellus
0.12
-
morphine cosubstrate NAD+ Cavia porcellus
0.15
-
normorphine cosubstrate NADP+ Cavia porcellus
0.4
-
ethylmorphine cosubstrate NAD+ Cavia porcellus
0.49
-
morphine cosubstrate NADP+ Cavia porcellus
0.54
-
Dihydrocodeine cosubstrate NAD+ Cavia porcellus
1.54
-
ethylmorphine cosubstrate NADP+ Cavia porcellus
2.11
-
Dihydrocodeine cosubstrate NADP+ Cavia porcellus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Cavia porcellus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
gel filtration Cavia porcellus

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-
Cavia porcellus Hartley
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, various chromatographic techniques Cavia porcellus

Reaction

Reaction Comment Organism Reaction ID
morphine + NAD(P)+ = morphinone + NAD(P)H + H+ dehydrogenation of the 6-hydroxy group of morphine derivatives having a 7,8-unsaturated bond Cavia porcellus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Cavia porcellus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.53
-
morphine, NAD+ Cavia porcellus

Storage Stability

Storage Stability Organism
2-4┬░C, 50 mM sodium phosphate buffer + 100 mM 2-mercaptoethanol, pH 6., 90% of the activity retained with both NAD+ and NADP+ for a week Cavia porcellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
codeine + NAD(P)+ oxidation at 80% the rate of morphine oxidation Cavia porcellus codeinone + NAD(P)H
-
?
codeine + NAD(P)+ oxidation at 80% the rate of morphine oxidation Cavia porcellus Hartley codeinone + NAD(P)H
-
?
dihydrocodeine + NADP+
-
Cavia porcellus dihydrocodeinone + NADPH
-
?
dihydrocodeine + NADP+
-
Cavia porcellus Hartley dihydrocodeinone + NADPH
-
?
ethylmorphine + NAD(P)+ oxidation at 51% the rate of morphine oxidation Cavia porcellus ?
-
?
morphine + NADP+
-
Cavia porcellus morphinone + NADPH + H+ production of morphinone demonstrated in vitro experiments ?
morphine + NADP+
-
Cavia porcellus Hartley morphinone + NADPH + H+ production of morphinone demonstrated in vitro experiments ?
nalorphine + NAD(P)+ best substrate, oxidation at 120% the rate of morphine oxidation Cavia porcellus (5alpha)-3-hydroxy-17-(prop-2-en-1-yl)-7,8-didehydro-4,5-epoxymorphinan-6-one + NAD(P)H + H+
-
?
nalorphine + NAD(P)+ best substrate, oxidation at 120% the rate of morphine oxidation Cavia porcellus Hartley (5alpha)-3-hydroxy-17-(prop-2-en-1-yl)-7,8-didehydro-4,5-epoxymorphinan-6-one + NAD(P)H + H+
-
?
normorphine + NAD(P)+ about 3 times better substrate than morphine Cavia porcellus ?
-
?
normorphine + NAD(P)+ about 3 times better substrate than morphine Cavia porcellus Hartley ?
-
?
trans-1,4-decalindiol + NADP+ about 30% of the activity observed with morphine Cavia porcellus ?
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
enzyme assay Cavia porcellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.1
-
with NADP+ as cofactor, in tricine-NaOH buffer Cavia porcellus
9.7
-
with NAD+ as cofactor, in glycine-NaCl-NaOH buffer Cavia porcellus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
in 50 mM sodium phosphate buffer + 100 mM 2-mercaptoethanol + NADP+ or NAD+ at least for a week stable at 2-4┬░C Cavia porcellus
8
-
in 50 mM sodium phosphate buffer + 100 mM 2-mercaptoethanol + NAD+ at least for a week stable at 2-4┬░C Cavia porcellus

Cofactor

Cofactor Comment Organism Structure
NAD(P)+ utilized both with nearly equal efficiency Cavia porcellus