Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas plecoglossicida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: EDTA | Pseudomonas plecoglossicida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.63 | - |
D-gluconate | pH 6, 25°C | Pseudomonas plecoglossicida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Pseudomonas plecoglossicida | 16020 | - |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 40% v/v, 32% residual activity | Pseudomonas plecoglossicida |
Ethanol | 40% v/v, complete loss of activity | Pseudomonas plecoglossicida |
hexane | 40% v/v, 39% residual activity | Pseudomonas plecoglossicida |
isopentanol | 40% v/v, complete loss of activity | Pseudomonas plecoglossicida |
Methanol | 40% v/v, complete loss of activity | Pseudomonas plecoglossicida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas plecoglossicida | A0A2H4PJM1 and A0A2H4PJM2 and A0A2H4PJM7 | A0A2H4PJM1 i.e gamma subunit, A0A2H4PJM2 i.e. FAD containing subunit, A0A2H4PJM7 i.e. cytochrome c subunit | - |
Pseudomonas plecoglossicida JUIM01 | A0A2H4PJM1 and A0A2H4PJM2 and A0A2H4PJM7 | A0A2H4PJM1 i.e gamma subunit, A0A2H4PJM2 i.e. FAD containing subunit, A0A2H4PJM7 i.e. cytochrome c subunit | - |
Purification (Comment) | Organism |
---|---|
native enzyme, via ultrasonification and ultracentrifugation, phase separation, ammonium sulfate precipitation, and DEAE-Sepharose and hydroxyapatite chromatography | Pseudomonas plecoglossicida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
90.71 | - |
pH 6, 35°C | Pseudomonas plecoglossicida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-gluconate + NADP+ | - |
Pseudomonas plecoglossicida | 2-dehydro-D-gluconate + NADPH + H+ | - |
? | |
D-gluconate + NADP+ | - |
Pseudomonas plecoglossicida JUIM01 | 2-dehydro-D-gluconate + NADPH + H+ | - |
? | |
additional information | no activity on other sugars and sugar acids | Pseudomonas plecoglossicida | ? | - |
- |
|
additional information | no activity on other sugars and sugar acids | Pseudomonas plecoglossicida JUIM01 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
More | enzyme consists of a gamma subunit, a flavoprotein subunit, and a cytochrome c subunit with molecular masses of about 27, 65, and 47 kDa, respectively | Pseudomonas plecoglossicida |
Synonyms | Comment | Organism |
---|---|---|
GADH | - |
Pseudomonas plecoglossicida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Pseudomonas plecoglossicida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pseudomonas plecoglossicida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | - |
no activity above | Pseudomonas plecoglossicida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas plecoglossicida |