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Literature summary for 1.1.1.215 extracted from

  • Wang, D.M.; Sun, L.; Sun, W.J.; Cui, F.J.; Gong, J.S.; Zhang, X.M.; Shi, J.S.; Xu, Z.H.
    A membrane-bound gluconate dehydrogenase from 2-keto-D-gluconic acid industrial producing strain Pseudomonas plecoglossicida JUIM01 Purification, characterization, and gene identification (2019), Appl. Biochem. Biotechnol., 188, 897-913 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas plecoglossicida

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: EDTA Pseudomonas plecoglossicida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.63
-
D-gluconate pH 6, 25°C Pseudomonas plecoglossicida

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Pseudomonas plecoglossicida 16020
-

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 40% v/v, 32% residual activity Pseudomonas plecoglossicida
Ethanol 40% v/v, complete loss of activity Pseudomonas plecoglossicida
hexane 40% v/v, 39% residual activity Pseudomonas plecoglossicida
isopentanol 40% v/v, complete loss of activity Pseudomonas plecoglossicida
Methanol 40% v/v, complete loss of activity Pseudomonas plecoglossicida

Organism

Organism UniProt Comment Textmining
Pseudomonas plecoglossicida A0A2H4PJM1 and A0A2H4PJM2 and A0A2H4PJM7 A0A2H4PJM1 i.e gamma subunit, A0A2H4PJM2 i.e. FAD containing subunit, A0A2H4PJM7 i.e. cytochrome c subunit
-
Pseudomonas plecoglossicida JUIM01 A0A2H4PJM1 and A0A2H4PJM2 and A0A2H4PJM7 A0A2H4PJM1 i.e gamma subunit, A0A2H4PJM2 i.e. FAD containing subunit, A0A2H4PJM7 i.e. cytochrome c subunit
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme, via ultrasonification and ultracentrifugation, phase separation, ammonium sulfate precipitation, and DEAE-Sepharose and hydroxyapatite chromatography Pseudomonas plecoglossicida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
90.71
-
pH 6, 35°C Pseudomonas plecoglossicida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-gluconate + NADP+
-
Pseudomonas plecoglossicida 2-dehydro-D-gluconate + NADPH + H+
-
?
D-gluconate + NADP+
-
Pseudomonas plecoglossicida JUIM01 2-dehydro-D-gluconate + NADPH + H+
-
?
additional information no activity on other sugars and sugar acids Pseudomonas plecoglossicida ?
-
-
additional information no activity on other sugars and sugar acids Pseudomonas plecoglossicida JUIM01 ?
-
-

Subunits

Subunits Comment Organism
More enzyme consists of a gamma subunit, a flavoprotein subunit, and a cytochrome c subunit with molecular masses of about 27, 65, and 47 kDa, respectively Pseudomonas plecoglossicida

Synonyms

Synonyms Comment Organism
GADH
-
Pseudomonas plecoglossicida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Pseudomonas plecoglossicida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Pseudomonas plecoglossicida

pH Range

pH Minimum pH Maximum Comment Organism
8
-
no activity above Pseudomonas plecoglossicida

Cofactor

Cofactor Comment Organism Structure
FAD
-
Pseudomonas plecoglossicida