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Literature summary for 1.1.1.215 extracted from

  • Rauch, B.; Pahlke, J.; Schweiger, P.; Deppenmeier, U.
    Characterization of enzymes involved in the central metabolism of Gluconobacter oxydans (2010), Appl. Microbiol. Biotechnol., 88, 711-718.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADPH below 0.01 mM, pH 7.0, 25┬░C Gluconobacter oxydans
0.051
-
NADH pH 7.0, 25┬░C Gluconobacter oxydans
5.16
-
2-oxo-D-gluconate pH 7.0, 25┬░C Gluconobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33200
-
6 * 33200, SDS-PAGE Gluconobacter oxydans
200000
-
PAGE Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxo-D-gluconate + NADPH + H+ Gluconobacter oxydans
-
D-gluconate + NADP+
-
?
2-oxo-D-gluconate + NADPH + H+ Gluconobacter oxydans 621H
-
D-gluconate + NADP+
-
?
2-oxo-L-gulonate + NADPH + H+ Gluconobacter oxydans
-
L-idonate + NADP+
-
?
2-oxo-L-gulonate + NADPH + H+ Gluconobacter oxydans 621H
-
L-idonate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans Q5FTU6
-
-
Gluconobacter oxydans 621H Q5FTU6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-D-gluconate + NADH + H+ enzyme shows dual cofactor specificity, being able to use both NADPH and NADH Gluconobacter oxydans D-gluconate + NAD+
-
?
2-oxo-D-gluconate + NADH + H+ enzyme shows dual cofactor specificity, being able to use both NADPH and NADH Gluconobacter oxydans 621H D-gluconate + NAD+
-
?
2-oxo-D-gluconate + NADPH + H+
-
Gluconobacter oxydans D-gluconate + NADP+
-
?
2-oxo-D-gluconate + NADPH + H+ enzyme shows dual cofactor specificity, being able to use both NADPH and NADH Gluconobacter oxydans D-gluconate + NADP+
-
?
2-oxo-D-gluconate + NADPH + H+
-
Gluconobacter oxydans 621H D-gluconate + NADP+
-
?
2-oxo-D-gluconate + NADPH + H+ enzyme shows dual cofactor specificity, being able to use both NADPH and NADH Gluconobacter oxydans 621H D-gluconate + NADP+
-
?
2-oxo-L-gulonate + NADPH + H+
-
Gluconobacter oxydans L-idonate + NADP+
-
?
2-oxo-L-gulonate + NADPH + H+
-
Gluconobacter oxydans 621H L-idonate + NADP+
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 33200, SDS-PAGE Gluconobacter oxydans

Synonyms

Synonyms Comment Organism
Gox0417
-
Gluconobacter oxydans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
48.2
-
2-oxo-D-gluconate pH 7.0, 25┬░C Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
NADH
-
Gluconobacter oxydans
NADPH
-
Gluconobacter oxydans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9.34
-
2-oxo-D-gluconate pH 7.0, 25┬░C Gluconobacter oxydans