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Literature summary for 1.1.1.215 extracted from

  • Pajaniappan, M.; Hall, J.E.; Cawthraw, S.A.; Newell, D.G.; Gaynor, E.C.; Fields, J.A.; Rathbun, K.M.; Agee, W.A.; Burns, C.M.; Hall, S.J.; Kelly, D.J.; Thompson, S.A.
    A temperature-regulated Campylobacter jejuni gluconate dehydrogenase is involved in respiration-dependent energy conservation and chicken colonization (2008), Mol. Microbiol., 68, 474-491.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information expression and activity of cj0414 and cj0415 are higher at 42┬░C than at 37┬░C Campylobacter jejuni

Application

Application Comment Organism
medicine ability of Campylobacter jejuni to use gluconate as an electron donor via GADH activity is an important metabolic characteristic that is required for full colonization of avian but not mammalian hosts Campylobacter jejuni

Cloned(Commentary)

Cloned (Comment) Organism
mutagenized cj0414 and cj0415 alleles are introduced into Campylobacter jejuni 81-176 Campylobacter jejuni

Protein Variants

Protein Variants Comment Organism
additional information cj0414 and cj0415 mutants lack GADH activity at either temperature. Cj0415 mutant does not exhibit gluconate-dependent respiration. Cj0415 mutant is defective in chicken colonization, but exhibits only a minor colonization defect in mice Campylobacter jejuni

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Campylobacter jejuni
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26900
-
sequence analysis, cj0414 Campylobacter jejuni
63700
-
sequence analysis, cj0415 Campylobacter jejuni

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
-
-
Campylobacter jejuni 81-176
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-gluconate + NADP+ wild-type exhibits gluconate-dependent respiration. Genes cj0414 and cj0415, orthologous to GADH, are co-transcribed. Campylobacter jejuni 81┬ľ176 does not use gluconate compounds as sole carbon sources. GADH plays a minor role in mouse colonization Campylobacter jejuni 2-dehydro-D-gluconate + NADPH + H+
-
?
D-gluconate + NADP+ wild-type exhibits gluconate-dependent respiration. Genes cj0414 and cj0415, orthologous to GADH, are co-transcribed. Campylobacter jejuni 81┬ľ176 does not use gluconate compounds as sole carbon sources. GADH plays a minor role in mouse colonization Campylobacter jejuni 81-176 2-dehydro-D-gluconate + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
GADH
-
Campylobacter jejuni
gluconate dehydrogenase
-
Campylobacter jejuni

Cofactor

Cofactor Comment Organism Structure
FAD
-
Campylobacter jejuni

pI Value

Organism Comment pI Value Maximum pI Value
Campylobacter jejuni cj0414, isoelectric focusing
-
5.5
Campylobacter jejuni cj0415, isoelectric focusing
-
8.8