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Literature summary for 1.1.1.215 extracted from

  • Toyama, H.; Furuya, N.; Saichana, I.; Ano, Y.; Adachi, O.; Matsushita, K.
    Membrane-bound, 2-keto-D-gluconate-yielding D-gluconate dehydrogenase from Gluconobacter dioxyacetonicus IFO 3271: molecular properties and gene disruption (2007), Appl. Environ. Microbiol., 73, 6551-6556.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant large subunits Gluconobacter frateurii

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound Gluconobacter frateurii 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ heme group of cytochrome C bound to the middle subunit Gluconobacter frateurii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE Gluconobacter frateurii
44000
-
1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE Gluconobacter frateurii
65000
-
1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE Gluconobacter frateurii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-gluconate + NADP+ Gluconobacter frateurii
-
2-dehydro-D-gluconate + NADPH
-
?
D-gluconate + NADP+ Gluconobacter frateurii IFO 3271
-
2-dehydro-D-gluconate + NADPH
-
?

Organism

Organism UniProt Comment Textmining
Gluconobacter frateurii A4PIB0 the large subunit is encoded by gene gndL, the small subunit by gene gndS, and the cytochrome C subunit by gene gndC; gene gndL encoding the large subunit of the enzyme
-
Gluconobacter frateurii IFO 3271 A4PIB0 the large subunit is encoded by gene gndL, the small subunit by gene gndS, and the cytochrome C subunit by gene gndC; gene gndL encoding the large subunit of the enzyme
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 67fold from membranes and bound to FAD by solubilization with 0.1% Triton X-100, anion chromatography, and three steps of hydrophobic interaction chromatography Gluconobacter frateurii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
137
-
purified native enzyme Gluconobacter frateurii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-gluconate + NADP+
-
Gluconobacter frateurii 2-dehydro-D-gluconate + NADPH
-
?
D-gluconate + NADP+ the large subunit is the catalytically active enzyme part, high substrate and regiospecificity Gluconobacter frateurii 2-dehydro-D-gluconate + NADPH
-
?
D-gluconate + NADP+
-
Gluconobacter frateurii IFO 3271 2-dehydro-D-gluconate + NADPH
-
?
D-gluconate + NADP+ the large subunit is the catalytically active enzyme part, high substrate and regiospecificity Gluconobacter frateurii IFO 3271 2-dehydro-D-gluconate + NADPH
-
?

Subunits

Subunits Comment Organism
trimer 1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE Gluconobacter frateurii

Synonyms

Synonyms Comment Organism
2-keto-D-gluconate-yielding D-gluconate dehydrogenase
-
Gluconobacter frateurii
FAD-GADH
-
Gluconobacter frateurii
GADH
-
Gluconobacter frateurii

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
assay at Gluconobacter frateurii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Gluconobacter frateurii

Cofactor

Cofactor Comment Organism Structure
cytochrome c bound by the 44 kDa subunit Gluconobacter frateurii
FAD bound to the enzyme's large 65 kDA subunit Gluconobacter frateurii
NADP+
-
Gluconobacter frateurii