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Literature summary for 1.1.1.215 extracted from

  • Yum, D-Y.; Bae, S-S.; Pan, J-G.
    Purification and characterization of the 2-ketoaldonate reductase from Brevibacterium ketosoreductum ATCC21914 (1998), Biosci. Biotechnol. Biochem., 62, 154-156.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADPH
-
Brevibacterium ketosoreductum
5
-
2,5-diketo-D-gluconate
-
Brevibacterium ketosoreductum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
2 * 35000, SDS-PAGE Brevibacterium ketosoreductum
72000
-
gel filtration Brevibacterium ketosoreductum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,5-diketo-D-gluconate + NADPH Brevibacterium ketosoreductum
-
5-keto-D-gluconate + NADP+
-
ir
2,5-diketo-D-gluconate + NADPH Brevibacterium ketosoreductum ATCC 21914
-
5-keto-D-gluconate + NADP+
-
ir
2-dehydro-D-gluconate + NADPH Brevibacterium ketosoreductum
-
D-gluconate + NADP+
-
?
2-dehydro-D-gluconate + NADPH Brevibacterium ketosoreductum ATCC 21914
-
D-gluconate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Brevibacterium ketosoreductum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-Toyopearl, Phenyl-Toyopearl, Blue-Sepharose, Sephacryl S-200 Brevibacterium ketosoreductum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
22.2
-
-
Brevibacterium ketosoreductum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-diketo-D-gluconate + NADPH
-
Brevibacterium ketosoreductum 5-keto-D-gluconate + NADP+
-
ir
2,5-diketo-D-gluconate + NADPH
-
Brevibacterium ketosoreductum ATCC 21914 5-keto-D-gluconate + NADP+
-
ir
2-dehydro-D-gluconate + NADPH
-
Brevibacterium ketosoreductum D-gluconate + NADP+
-
?
2-dehydro-D-gluconate + NADPH 11.8% of activity with 2,5-diketo-D-gluconate Brevibacterium ketosoreductum D-gluconate + NADP+
-
?
2-dehydro-D-gluconate + NADPH
-
Brevibacterium ketosoreductum ATCC 21914 D-gluconate + NADP+
-
?
2-dehydro-D-gluconate + NADPH 11.8% of activity with 2,5-diketo-D-gluconate Brevibacterium ketosoreductum ATCC 21914 D-gluconate + NADP+
-
?
2-keto-L-gulonate + NADPH 10.8% of activity with 2,5-diketo-D-gluconate Brevibacterium ketosoreductum L-idonate + NADP+
-
?
2-keto-L-gulonate + NADPH 10.8% of activity with 2,5-diketo-D-gluconate Brevibacterium ketosoreductum ATCC 21914 L-idonate + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE Brevibacterium ketosoreductum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Brevibacterium ketosoreductum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7.5 35% of activity left after incubation at pH 8.4 for 2 h at 30Ā°C, no remaining activity below pH 5.2 or above pH 10.0 Brevibacterium ketosoreductum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Brevibacterium ketosoreductum
NADPH
-
Brevibacterium ketosoreductum