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Literature summary for 1.1.1.215 extracted from

  • Chiyonobu, T.; Shinagawa, E.; Adachi, O.; Ameyama, M.
    Purification, crystallization and properties of 2-ketogluconate reductase from Acetobacter rancens (1976), Agric. Biol. Chem., 40, 175-184.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Acetobacter pasteurianus

General Stability

General Stability Organism
2-mercaptoethanol stabilizes Acetobacter pasteurianus
dialysis, stable to Acetobacter pasteurianus

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
Acetobacter pasteurianus
Co2+
-
Acetobacter pasteurianus
Hg2+ complete inhibition Acetobacter pasteurianus
additional information not inhibited by monoiodoacetate Acetobacter pasteurianus
oxalate
-
Acetobacter pasteurianus
oxamate slight inhibition of reverse reaction Acetobacter pasteurianus
pyruvate weak Acetobacter pasteurianus
Tartronate slight inhibition of reverse reaction Acetobacter pasteurianus
Zn2+
-
Acetobacter pasteurianus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADPH + 5-keto-D-gluconate Acetobacter pasteurianus
0.062
-
NADPH + 2-keto-D-galactonate Acetobacter pasteurianus
0.065
-
Hydroxypyruvate
-
Acetobacter pasteurianus
0.083
-
NADP+ + D-gluconate Acetobacter pasteurianus
0.11
-
NADPH + 2-keto-L-gulonate Acetobacter pasteurianus
0.14
-
NADPH + hydroxypyruvate Acetobacter pasteurianus
0.38
-
glyoxylate
-
Acetobacter pasteurianus
0.71
-
NADPH cosubstrate glyoxylate Acetobacter pasteurianus
0.86
-
5-Keto-D-gluconate
-
Acetobacter pasteurianus
2.4
-
D-gluconate
-
Acetobacter pasteurianus
16
-
2-keto-D-galactonate
-
Acetobacter pasteurianus
91
-
2-keto-L-gulonate
-
Acetobacter pasteurianus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Acetobacter pasteurianus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal requirement Acetobacter pasteurianus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
8 * 15000, SDS-PAGE Acetobacter pasteurianus
120000
-
gel filtration Acetobacter pasteurianus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydro-D-gluconate + NADPH Acetobacter pasteurianus
-
D-gluconate + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Acetobacter pasteurianus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Acetobacter pasteurianus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
336
-
-
Acetobacter pasteurianus

Storage Stability

Storage Stability Organism
-20°C, stable in the presence of sulfhydryl agents and ammonium sulfate Acetobacter pasteurianus
0°C, stable for at least a month in phosphate buffer Acetobacter pasteurianus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-D-gluconate + NADPH
-
Acetobacter pasteurianus D-gluconate + NADP+
-
r
2-keto-L-gulonate + NADPH
-
Acetobacter pasteurianus L-idonate + NADP+
-
r
5-keto-D-gluconate + NADPH poor substrate Acetobacter pasteurianus D-gluconate + NADP+
-
?
acetaldehyde + NADPH + H+ poor substrate Acetobacter pasteurianus ethanol + NADP+
-
?
D-galactonate + NADP+
-
Acetobacter pasteurianus 2-dehydro-D-galactonate + NADPH
-
r
glyoxal + NADPH reduction at 33% the rate of 2-ketogluconate reduction Acetobacter pasteurianus ?
-
?
glyoxylate + NADPH reduction at 350% the rate of 2-ketogluconate reduction Acetobacter pasteurianus glycolate + NADP+
-
?
hydroxypyruvate + NADPH reduction at 733% the rate of 2-keto-gluconate reduction Acetobacter pasteurianus 2,3-dihydroxypropanoate + NADP+
-
?
L-idonate + NADP+
-
Acetobacter pasteurianus 2-keto-L-idonate + NADPH
-
?
pyruvate + NADPH + H+ reduction at 7% the rate of 2-ketogluconate reduction Acetobacter pasteurianus lactate + NADP+
-
?

Subunits

Subunits Comment Organism
octamer 8 * 15000, SDS-PAGE Acetobacter pasteurianus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Acetobacter pasteurianus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 55 about half-maximal activity at 40°C and 55°C Acetobacter pasteurianus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
stable for 10 min Acetobacter pasteurianus
60
-
rapid inactivation Acetobacter pasteurianus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
reduction of 2-ketogluconate Acetobacter pasteurianus
12
-
-
Acetobacter pasteurianus

pH Range

pH Minimum pH Maximum Comment Organism
5 9.2 about half-maximal activity at pH 5.0 and 9.2, reduction of ketogluconate Acetobacter pasteurianus
10.8 12.5 about half-maximal activity at pH 10.8 and 12.5, oxidation of gluconate Acetobacter pasteurianus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
stable for a week in the cold, increasing inactivation below and above, stable for a month or more with additional 2-mercaptoethanol Acetobacter pasteurianus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Acetobacter pasteurianus
NADPH
-
Acetobacter pasteurianus