Crystallization (Comment) | Organism |
---|---|
- |
Acetobacter pasteurianus |
General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes | Acetobacter pasteurianus |
dialysis, stable to | Acetobacter pasteurianus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Acetobacter pasteurianus | |
Co2+ | - |
Acetobacter pasteurianus | |
Hg2+ | complete inhibition | Acetobacter pasteurianus | |
additional information | not inhibited by monoiodoacetate | Acetobacter pasteurianus | |
oxalate | - |
Acetobacter pasteurianus | |
oxamate | slight inhibition of reverse reaction | Acetobacter pasteurianus | |
pyruvate | weak | Acetobacter pasteurianus | |
Tartronate | slight inhibition of reverse reaction | Acetobacter pasteurianus | |
Zn2+ | - |
Acetobacter pasteurianus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADPH | + 5-keto-D-gluconate | Acetobacter pasteurianus | |
0.062 | - |
NADPH | + 2-keto-D-galactonate | Acetobacter pasteurianus | |
0.065 | - |
Hydroxypyruvate | - |
Acetobacter pasteurianus | |
0.083 | - |
NADP+ | + D-gluconate | Acetobacter pasteurianus | |
0.11 | - |
NADPH | + 2-keto-L-gulonate | Acetobacter pasteurianus | |
0.14 | - |
NADPH | + hydroxypyruvate | Acetobacter pasteurianus | |
0.38 | - |
glyoxylate | - |
Acetobacter pasteurianus | |
0.71 | - |
NADPH | cosubstrate glyoxylate | Acetobacter pasteurianus | |
0.86 | - |
5-Keto-D-gluconate | - |
Acetobacter pasteurianus | |
2.4 | - |
D-gluconate | - |
Acetobacter pasteurianus | |
16 | - |
2-keto-D-galactonate | - |
Acetobacter pasteurianus | |
91 | - |
2-keto-L-gulonate | - |
Acetobacter pasteurianus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Acetobacter pasteurianus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal requirement | Acetobacter pasteurianus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
8 * 15000, SDS-PAGE | Acetobacter pasteurianus |
120000 | - |
gel filtration | Acetobacter pasteurianus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-D-gluconate + NADPH | Acetobacter pasteurianus | - |
D-gluconate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetobacter pasteurianus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acetobacter pasteurianus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
336 | - |
- |
Acetobacter pasteurianus |
Storage Stability | Organism |
---|---|
-20°C, stable in the presence of sulfhydryl agents and ammonium sulfate | Acetobacter pasteurianus |
0°C, stable for at least a month in phosphate buffer | Acetobacter pasteurianus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-D-gluconate + NADPH | - |
Acetobacter pasteurianus | D-gluconate + NADP+ | - |
r | |
2-keto-L-gulonate + NADPH | - |
Acetobacter pasteurianus | L-idonate + NADP+ | - |
r | |
5-keto-D-gluconate + NADPH | poor substrate | Acetobacter pasteurianus | D-gluconate + NADP+ | - |
? | |
acetaldehyde + NADPH + H+ | poor substrate | Acetobacter pasteurianus | ethanol + NADP+ | - |
? | |
D-galactonate + NADP+ | - |
Acetobacter pasteurianus | 2-dehydro-D-galactonate + NADPH | - |
r | |
glyoxal + NADPH | reduction at 33% the rate of 2-ketogluconate reduction | Acetobacter pasteurianus | ? | - |
? | |
glyoxylate + NADPH | reduction at 350% the rate of 2-ketogluconate reduction | Acetobacter pasteurianus | glycolate + NADP+ | - |
? | |
hydroxypyruvate + NADPH | reduction at 733% the rate of 2-keto-gluconate reduction | Acetobacter pasteurianus | 2,3-dihydroxypropanoate + NADP+ | - |
? | |
L-idonate + NADP+ | - |
Acetobacter pasteurianus | 2-keto-L-idonate + NADPH | - |
? | |
pyruvate + NADPH + H+ | reduction at 7% the rate of 2-ketogluconate reduction | Acetobacter pasteurianus | lactate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 15000, SDS-PAGE | Acetobacter pasteurianus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Acetobacter pasteurianus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 55 | about half-maximal activity at 40°C and 55°C | Acetobacter pasteurianus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
stable for 10 min | Acetobacter pasteurianus |
60 | - |
rapid inactivation | Acetobacter pasteurianus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
reduction of 2-ketogluconate | Acetobacter pasteurianus |
12 | - |
- |
Acetobacter pasteurianus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9.2 | about half-maximal activity at pH 5.0 and 9.2, reduction of ketogluconate | Acetobacter pasteurianus |
10.8 | 12.5 | about half-maximal activity at pH 10.8 and 12.5, oxidation of gluconate | Acetobacter pasteurianus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
stable for a week in the cold, increasing inactivation below and above, stable for a month or more with additional 2-mercaptoethanol | Acetobacter pasteurianus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Acetobacter pasteurianus | |
NADPH | - |
Acetobacter pasteurianus |