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Literature summary for 1.1.1.214 extracted from

  • Pei, X.; Wang, J.; Zheng, H.; Cheng, P.; Wu, Y.; Wang, A.; Su, W.
    Highly efficient asymmetric reduction of ketopantolactone to D-(-)-pantolactone by Escherichia coli cells expressing recombinant conjugated polyketone reductase and glucose dehydrogenase in a fed-batch biphasic reaction system (2020), React. Chem. Eng., 5, 531-538 .
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Application

Application Comment Organism
synthesis whole-cell biotransformation process to produce D-pantolactone in a biphasic reaction system. Recombinant CPR and glucose dehydrogenase are co-expressed in Escherichia coli to simultaneously achieve the synthesis of D-PL and the regeneration of NADPH. Presence of 15% dichloromethane significantly inhibits the hydrolysis of ketopantolactone. In a fed-batch system, the D-pantolactone concentration reaches 0.77 mol per l in the reaction mixture at 7 h, and its enantiomeric excess is 99% Candida dubliniensis

Organism

Organism UniProt Comment Textmining
Candida dubliniensis B9WJQ5
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Candida dubliniensis CD36 B9WJQ5
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-pantolactone + NADP+
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 Candida dubliniensis 2-dehydropantolactone + NADPH + H+
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 ?
(R)-pantolactone + NADP+
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 Candida dubliniensis CD36 2-dehydropantolactone + NADPH + H+
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 ?

Synonyms

Synonyms Comment Organism
CPR-C2
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 Candida dubliniensis